[Yakult] Enzymes for Protoplast Preparation
Cellulase Onozuka™ R-10, Cellulase Onozuka™ RS and Macerozyme™ R-10 are enzymes supplied by Yakult Pharmaceutical Industry Co. Ltd. and used for protoplast preparation. By using these products, protoplasts can be prepared with relative ease.
Cellulase Onozuka™ R-10 / Cellulase Onozuka™ RS
These products degrade natural cellulose and dissolve plant cell walls. Used in isolating protoprasts from plant mesophyll and cultured cells.
Macerozyme™ R-10
This product contains pectinase and hemicellulase at high titers and degrades plant tissues to single cells.
Summary of Protoplast Preparation
If plant tissue is separated into single cells with plant tissue macerating enzymes and then treated with plant tissue degrading enzymes, the plant tissue becomes a single cell, If it is further and treated with cell wall degrading enzymes, the cell wall dissolves leaving spherical cells encased in the cell membrane, known as protoplasts. These are used in cell engineering for a variety of different purposes, including; protoplasts from different types of plants being fused together to create new plants, or new genes being introduced into cells to give plants new properties.
Product Information
Product Lineup
| 10 g type (conventional product) | Vial type | |||||
|---|---|---|---|---|---|---|
| Product Number | 636-01441 | 639-01431 | 635-02631 | 635-49751 | 632-49761 | 639-49771 |
| Product Name | Cellulase Onozuka™ R-10 |
Cellulase Onozuka™ RS |
Macerozyme™ R-10 |
Cellulase Onozuka™ R-10 |
Cellulase Onozuka™ RS |
Macerozyme™ R-10 |
| Enzyme Activity | More than 10,000 u/g | More than 16,000 u/g | More than 3,000 u/g | More than 10,000 u/g | More than 16,000 u/g | More than 3,000 u/g |
| Packaging |  |
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| 10g/brown bottle | 5 vials/box | |||||
| Optimal pH | pH 4.0 to 5.0 | pH5.0 to 6.0 | pH 4.0 to 5.0 | pH5.0 to 6.0 | ||
| Optimal Temperature | 40 to 55 ℃ | 40 to 50 ℃ | 40 to 55 ℃ | 40 to 50 ℃ | ||
Excellent Solubility of Vial type
Improved solubility compared to the conventional product, enhances workability and operability.
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10 g type
Vial type
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.