Uniform and Reproducible Spheroid Formation
High-Quality 3D Cell Culture Tools Made in Japan PrimeSurface™
PrimeSurface™ cultureware are ultra low attachment (ULA) plate and dish that promote scaffold free, self assembly of spheroid formation. The plates are pre-coated with unique ultra hydrophilic polymer that enables spontaneous spheroid formation of uniform size and shape. PrimeSurface™ has been used for many years in a wide range of research fields, including regenerative medicine and cancer research.
Product Formats
PrimeSurface™ is available in multiple formats to accommodate diverse experimental requirements.
Plates
- 96-Well (U-Bottom, V-Bottom, White U-Bottom, Spindle-Bottom)
- 96-Well Slit-Well
- 24-Well Flat-Bottom
- 384-Well (U-Bottom, White U-Bottom)
Dishes
- 35 mm, 60 mm, 90 mm
For high-throughput screening (HTS), both clear and white 384-well formats are available.
96 Slit-well Plate
PrimeSurface™ 96 Slit-Well Plates enable simultaneous media exchange across all 96 wells using a single dispensing or aspiration step.
- More than 80% reduction in pipetting time
- Improved workflow efficiency
- Reduced risk of spheroid damage
- Enhanced consistency during media changes
Features
- Ideal tool for uniform spheroid cell culture formation.
- The uniform spheroid cell culture could be effective formed due to the adhesion of cell is inhibited by our unique surface coating technique of PrimeSurface product.
- Ideal tool for differentiation study
- Inducer reagent for differentiation can be applied after EB body formation of ES cells.
- The chemical bonding of ultra-hydrophilic coating polymer on our product is stable culture surface which makes no elution of chemicals from substrate.
- Ideal for differentiation and stimulation response studies.
Comparison data3D spheroid culture using HepG2 cell line
| Cell line | : | HepG2 |
| Seeding density | : | 1000 cells/well |
| Medium | : | DMEM+10%FBS |
| Culture period | : | 3 days |
| Plate | : | 96 well U plates for 3D culturing from different companies |
Classification based on microscopic images
Number of Spheroids
- [Grade 1]
![Number of Spheroids[Grade 1]](../../us/category/images/02026_img05.jpg)
Single spheroid from single well - [Grade 2]
![Number of Spheroids[Grade 2]](../../us/category/images/02026_img06.jpg)
One main spheroid and one small satellite from one well - [Grade 3]
![Number of Spheroids[Grade 3]](../../us/category/images/02026_img07.jpg)
3 or more spheroids or badly deformed ones from one well - [Grade 4]
![Number of Spheroids[Grade 4]](../../us/category/images/02026_img08.jpg)
No spheroid formation
| Manufacturer | Product | Grade 1 | Grade 2 | Grade 3 | Grade 4 |
|---|---|---|---|---|---|
| Company A | Product A | 23 | 3 | 70 | 0 |
| Company B | Product B | 65 | 27 | 4 | 0 |
| Company C | Product C | 83 | 8 | 5 | 0 |
| Company D | Product D | 88 | 6 | 1 | 1 |
| Company E | Product E | 95 | 1 | 0 | 0 |
| Sumitomo Bakelite Co., Ltd. | PrimeSurface™ | 96 | 0 | 0 | 0 |
Remark
Values and images used in this application note is measured values at Sumitomo Bakelite Co., Ltd., not the values for guaranteeing. In addition, these data do not gurantee the product performance under any conditions.
Values and images used in this application note is measured values at Sumitomo Bakelite Co., Ltd., not the values for guaranteeing. In addition, these data do not gurantee the product performance under any conditions.
Applications
- Generation of Human iPS Cell-Derived Kidney Organoids and Their Application in Drug Discovery Research
- Stimulus effects Evaluation
- Blood-brain barrier (BBB)
- REPRODUCIBLE HUMAN CORTICAL ORGANOIDS
- 3D Kidney model for Drug Discovery Research
Brain organoids generation from iPS cells
V bottom plate(MS-9096VZ) is suitable for human iPS cell aggregation for efficient self organization and organoid formation.
【Cerebellar】
【Cerebrum】
【Retina】
Using PrimeSurface plates enables the production of uniform spheroids of the same size
Remark
Values and images used in this application note is measured values at Sumitomo Bakelite Co., Ltd., not the values for guaranteeing. In addition, these data do not gurantee the product performance under any conditions.
Values and images used in this application note is measured values at Sumitomo Bakelite Co., Ltd., not the values for guaranteeing. In addition, these data do not gurantee the product performance under any conditions.
Lineup
| Cat. No | Product | Well | Color | Well Shape (Culture area) |
Volume | Qty/Pk | Qty/Cs |
|---|---|---|---|---|---|---|---|
| MS-9096UZ | PrimeSurface 96U | 96 | Clear | U-bottom | 0.3 mL | 1 | 20 |
| MS-9096VZ | PrimeSurface 96V | 96 | Clear | V-bottom | 0.3 mL | 1 | 20 |
| MS-9096WZ | PrimeSurface 96U White Plate | 96 | White | U-bottom | 0.3 mL | 1 | 20 |
| MS-9096MZ | PrimeSurface 96M | 96 | Clear | Spindle-bottom | 0.2 mL | 1 | 20 |
| MS-9096S | PrimeSurface 96 Slit‑Well Plate | 96 | Clear | Spindle-bottom | 0.3 mL | 1 | 20 |
| MS-90240 | PrimeSurface Plate 24F | 24 | Clear | Flat (1.8 cm2) | 3.4 mL | 1 | 10 |
| MS-9384U | PrimeSurface 384U | 384 | Clear | U-bottom | 0.1 mL | 1 | 20 |
| MS-9384W | PrimeSurface 384U White Plate | 384 | White | U-bottom | 0.1 mL | 1 | 20 |
| MS-90350 | PrimeSurface Dish 35 | - | Clear | Flat (9 cm2) | - | 5 | 50 |
| MS-90600 | PrimeSurface Dish 60 | - | Clear | Flat (21 cm2) | - | 10 | 120 |
| MS-90900 | PrimeSurface Dish 90 | - | Clear | Flat (57 cm2) | - | 10 | 50 |
Q&A
- How to control the size of the spheroids?
- The diameter of the spheroids can be controlled by changing the initial seeding density.
- What is the difference between PrimeSurface flat dish and culture plate?
- PrimeSurface flat dishes (35, 60 and 90 mm) are flat bottoms and randomly generate multiple spheroids with different size whereas PrimeSurface culture plates (96 and 384 wells) were designed to generate single and uniform sized spheroids in every well.
- What are the dimensions of the plate for automation programing?
- You can find the dimensions of PrimSurface plate from website(see Multi Plate Well Size). PrimeSurface 96 slit well plate and 384 plate adhere to the standard ANSI/SBS footprint dimensions.
- How do I change media when necessary?
- A 50/50 media exchange is recommended. For example, 96-well plate with a 100 μL starting volume, remove 50 μL of spent media from the wells, and replace with 50 μL of fresh media.
- Are there any special note when using the plate?
- As the surface of each well is coated with hydrophilic polymer, during seeding, make sure pipet tips do not scratch the bottom or sides of the wells. Cells may attach to the well if there is a scratch.
- Can I use a centrifuge to spin down the cells?
- Centrifugal force of 3000 g was used in Sumitomo Bakelite’s internal data but this is highly variable and depends on the cell type and not a guaranteed value.
References
- High-throughput differentiation of human blood vessel organoids reveals overlapping and distinct functions of the cerebral cavernous malformation proteins
- Mathematical modeling of multicellular tumor spheroids quantifies inter-patient and intra-tumor heterogeneity
- Transcriptional Up-Regulation of FBXW7 by KCa1.1 K+ Channel Inhibition through the Nrf2 Signaling Pathway in Human Prostate Cancer LNCaP Cell Spheroid Model
- FSP1 is a predictive biomarker of osteosarcoma cells’ susceptibility to ferroptotic cell death and a potential therapeutic target
- Hepatocytes differentiate into intestinal epithelial cells through a hybrid epithelial/mesenchymal cell state in culture
- Anti-Mesothelin CAR-NK cells as a novel targeted therapy against cervical cancer
- Personalized Vascularized Models of Breast Cancer Desmoplasia Reveal Biomechanical Determinants of Drug Delivery to the Tumor
- Three-dimensional culture of human proximal tubular epithelial cells for an in vitro evaluation of drug-induced kidney injury
- Long-term viable chimeric nephrons generated from progenitor cells are a reliable model in cisplatin-induced toxicity
- Generation of multilineage liver organoids with luminal vasculature and bile ducts from human pluripotent stem cells via modulation of Notch signaling
- In vitro hair follicle growth model for drug testing
- Comparison of the effects of oxidative and inflammatory stresses on rat chondrocyte senescence
- Proteomics and disease network associations evaluation of environmentally relevant Bisphenol A concentrations in a human 3D neural stem cell model
- Hypoxia inducible factor-1α promotes trichogenic gene expression in human dermal papilla cells
- ASPSCR1::TFE3 orchestrates the angiogenic program of alveolar soft part sarcoma
- Enhanced chondrogenic differentiation of iPS cell-derived mesenchymal stem/stromal cells via neural crest cell induction for hyaline cartilage repair
- PrimeSurface™ Stem Cell Differentiation Research Publication List
- Application Examples in Stem Cell Research (PDF 995KB)
- PrimeSurface™ Other application Publication List
Product List
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PrimeSurface 96Well Plate
PrimeSurface 96 Slit-well Plate
PrimeSurface 384Well Plate
PrimeSurface 24Well Flat bottom Plate
PrimeSurface Dish
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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