deColorizing Solution

• Allows decolorization for H&E and MT staining
• Simple procedure, only requiring immersion in this reagent
• Allows re-staining, such as antibody staining and H&E staining, after decolorization

Decolorization using mouse brain (hippocampus)

Data by courtesy of Dr. Aisa Ozawa and Dr. Motoharu Sakaue, Azabu University

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  H&E Staining

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  Decolorization

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  Decolorization followed by antibody staining(Iba1、DAPI)

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  Control (no decolorization)

H&E stain (H&E → decolorization → H&E)

Mouse liver

H&E stain (H&E → decolorization → antibody staining [PCNA])

MT stain (MT → decolorization → H&E)

1. Dilute each reagent 10-fold with distilled water. (Prepare the volume to just cover a slide staining tray.)
   deColorizing Solution 1 diluent → Solution 1
   deColorizing Solution 2 diluent → Solution 2
2. Remove the cover glass from the sample to be decolorized, and hydrate.
3. In the case of H&E stain decolorization, confirm that colorization with water-soluble dyes has disappeared, and immerse in Solution 1 at room temperature for one hour to overnight.
4. In the case of MT stain decolorization, perform the procedure in 3 and immerse in Solution 2 at room temperature for one hour to overnight.
5. After confirming the decolorization of HE and MT staining, immerse in distilled water and wash 3 times x 5 minutes each.
6. Follow each institutional protocols for re-staining.

*The stainability of eosin or other dyes after decolorization may decrease if decolorization time is prolonged. In that case, time should be adjusted.