[NIPPON GENE] Agarose

Product Name	S、S＜Tablet＞	HS	L	H	XP	21	X
Feature/Application	Standard	Blotting, etc.	Recovering DNA	High gel strength	Recovering DNA, 3 % gel	3% gel	3% gel
Separation range (kbp)	0.5 - 30	0.5 - 30	0.5 - 30	1 - 200	0.01 - 20	0.01 - 1.0	0.01 - 1.0
Concentration range	0.5 - 2%	0.5 - 2%	0.5 - 2%	0.2 - 1%	1 - 4%	2 - 5%	2 - 6%
No weighing required	0.5 g/tablet*1					3g*2
Gel strength	≥1,200g/cm2 (1.5%)	≥1,600g/cm2 (1.5%)	≥450g/cm2 (1.5%)	≥2,600g/cm2 (1.5%)	≥450g/cm2 (1.5%)	≥800g/cm2 (3%)	≥1,200g/cm2 (4%)
Melting point	≤90°C (1.5%)	≤93°C (1.5%)	≤65°C (1.5%)	boil (1.5%)	≤65°C (1.5%)	≤85°C (3%)	≤92°C (4%)
Gelation temperature	37～39°C (1.5%)	37～39°C (1.5%)	≤30°C (1.5%)	37～39°C (1.5%)	≤30°C (1.5%)	34～38°C (3%)	31～39°C (4%)

*1 Agarose S is a tablet containing 0.5 g of Agarose S.
*2 Stick type (3 g) is also available for Agarose 21.

1. Gel strength
2. Melting point
3. Gelation temperature
4. Sulfate content (SO₄)
5. Water content
6. Electroendosmosis (-Mr)
7. No detection of DNase and RNase
8. Check for recovered DNA
   
   1. Restriction enzyme cleavage
   2. Ligation with T4 DNA ligase
   3. Transformation of E. coli with ligated DNA (e.g., pBR322)