LC Derivatizing Reagent
Major Types and Applications
In order to achieve high sensitivity and selectivity of the analyte in high performance liquid chromatography (HPLC), labeling is performed with a derivatizing reagent appropriate for the detection method.
Methods include the pre-column method, in which the analytes are labeled in advance, and the post-column method, in which the analytes are labeled with derivatizing reagents after HPLC separation.
| Code No. | Product Name | Detection | Derivatization | Functional group |
|---|---|---|---|---|
| (L08379) | 4-Amino-3-penten-2-one | Fluorescence | post-column | Aldehyde |
| 013-09991 | N-(4-Anilinophenyl)maleimide | ECD | SH group | |
| 017-12101 | 1-Anthroyl Cyanide | Fluorescence | pre-column | OH group |
| 531-20721 | BCA Protein Assay Kit | Visible | post-column | Proteins, Peptides |
| 028-07781 | Bis(2,4-dinitrophenyl)Oxalate (DNPO) | Fluorescence | pre-column | Chemiluminescence (H2O2) |
| 048-20101 | Bis[2-(3,6-dioxaoctyloxycarbonyl)-4-nitrophenyl]Oxalate (DOPO) | Fluorescence | pre-column | Chemiluminescence (H2O2) |
| 204-09161 | Bis[4-nitro-2-(3,6,9-trioxadecyloxycarbonyl)-phenyl]Oxalate (TDPO) | Fluorescence | pre-column | Chemiluminescence (H2O2) |
| 028-07421 | Bis（2,4,6-trichlorophenyl）Oxalate（TCPO） | Fluorescence | pre-column | Chemiluminescence (H2O2) |
| 349-04021 | 4-Bromomethyl-7-methoxycoumarin (Br-Mmc) | Fluorescence | pre-column | Carboxylic acid |
| 048-18251 | Dansyl Chloride | Fluorescence | pre-column | Proteins, Peptides |
| 042-18254 | ||||
| 044-18253 | ||||
| 347-05541 | 1,2-Diamino-4,5-methylenedioxybenzene Dihydrochloride (MDB) | Fluorescence | pre-column | α-Keto acid |
| 343-05543 | ||||
| 049-02632 | p-Dimethylaminobenzaldehyde | Fluorescence | pre-column | Guanidine compound |
| 043-02635 | ||||
| 049-23671 | 6,7-Dimethoxy-1-methyl-2(1H)-quinoxalinone-3-propionohydrazide (DMEQ-Hydrazide) | Fluorescence | pre-column | Carboxylic acid for fluorescent labeling |
| 349-03661 | Fluorescein Isothiocyanate Isomer I (FITC-I) | Fluorescence | pre-column | Amino acid |
| 343-03664 | ||||
| 342-04751 | 4-Fluoro-7-nitrobenzofurazan (NBD-F) | Fluorescence | pre-column | Amino acid |
| 348-04753 | ||||
| 341-05441 | 4-Fluoro-7-sulfamoylbenzofurazan (ABD-F) | Fluorescence | pre-column | SH group |
| 347-05443 | ||||
| 342-05111 | 4-Fluoro-7-sulfobenzofurazan Ammonium Salt (SBD-F) | Fluorescence | pre-column | |
| 348-05113 | ||||
| 077-02751 | 2,3,4,6-Tetra-O-acetyl-beta-D-glucopyranosyl Isothiocyanate (GITC) | UV | pre-column | Optical resolution of amino group |
| 073-02753 | ||||
| 148-05911 | 2-(2,3-Naphthalimino)ethyl Trifluoromethane- sulfonate (NE-OTf) | Fluorescence, UV | pre-column | Carboxylic acid |
| 166-11301 | 9,10-Phenanthrenequinone | Fluorescence | pre-column | Guanidine compound |
| 162-11303 | ||||
| 161-09261 | o-Phthalaldehyde | Fluorescence | post-column | Alkaloids, Nitrogen compounds |
| 167-09263 | ||||
| 169-09262 | ||||
| 165-09264 | ||||
| 345-05221 | Sulforhodamine 101 Acid Chloride | Fluorescence | pre-column | Proteins, Amino acid |
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