Xylene substitutes

PathoClean

Data by courtesy of Kasumigaura Medical Center, National Hospital Organization

In Japan, with the partial revision of Order for Enforcement of the Industrial Safety and Health Act, xylene is now subject to the "Ordinance on Prevention of Organic Solvent Poisoning" and women cannot work in an environment that contains more than a controlled concentration of 50 ppm.

Use this product to replace xylene.

Examples of comparative staining with xylene and PathoClean (Human)

H&E staining (stomach)

  • Left: Xylene
  • Right:PathoClean

H&E staining (fallopian tube)

  • Left: Xylene
  • Right:PathoClean

Elastica van Gieson staining (lung)

  • Left: Xylene
  • Right:PathoClean

Periodic acid-Schiff staining (kidney)

  • Left: Xylene
  • Right:PathoClean

Masson's trichrome staining (liver)

  • Left: Xylene
  • Right:PathoClean

Data by courtesy of Kasumigaura Medical Center, National Hospital Organization

Examples of comparative staining with xylene and PathoClean (Mouse)

Immunohistochemical staining-PCNA staining

Material: Mouse breast cancer, formalin-fixed

  • Left: Xylene
  • Right:PathoClean

H&E staining

Material: Mouse breast cancer, formalin-fixed

  • Left: Xylene
  • Right:PathoClean

Data by courtesy of Dr. Masaaki Shibata, Department of Anatomy and Cell Biology , Division of Life Sciences, Osaka Medical College

Features

Xylene or other compounds that are compatible with alcohol and paraffin are commonly used as an intermediate agent. PathoClean can be used instead of these compounds in the process of deparaffinization and permeation.

*We recommend PathoMount (Product Number 164-28492, 168-28495) or UI Sol (UI Kasei) as a mounting agent for PathoClean. Please be aware that white turbidity may occur when Marinol (Muto Pure Chemicals Co., Ltd.) is used.

Examples of H&E staining protocol
Step Procedure Time
1 PathoClean (1st) 5 min.
2 PathoClean (2nd) 3 min.
3 PathoClean (3rd) 2 min.
4 PathoClean (4th) 1 min.
5 100% Ethanol (1st) 1 min.
6 100% Ethanol (2nd) 1 min.
7 95% Ethanol 1 min.
8 Wash (Water) 2 min.
9 Hematoxylin 6 min.
10 Wash (Water) 4 min.
11 0.1% Hydrochloric Acid 1 sec.
12 Wash (Water) 5 min.
13 Eosin 5 min.
14 Distilled water 2 sec.
15 100% Ethanol (1st) 30 sec.
16 100% Ethanol (2nd) 30 sec.
17 100% Ethanol (3rd) 1 min.
18 100% Ethanol (4th) 1 min.
19 100% Ethanol (5th) 1 min.
20 100% Ethanol (6th) 1 min.
21 PathoClean (1st) 1 min.
22 PathoClean (2nd) 1 min.
23 PathoClean (3rd) 1 min.
24 PathoClean (4th) 10 sec.
25 PathoMount (mounting agents) -

Product List

  • Open All
  • Close All

PathoClean

for Pathology Research

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at RT.

Comparison
Product Number
Package Size
Price
Inventory
Distributor
161-28321
1L
Inquire

In stock in Japan

Distributor
169-28327
18L
Inquire

In stock in Japan

Now Loading...

Related Product List

  • Open All
  • Close All

Now Loading...

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

+49-2131-311-271

Reception hours: Mon-Fri 9:00 - 15:00 (CET)For other hours than the above, please contact us via the inquiry form.