PathoClean
In Japan, with the partial revision of Order for Enforcement of the Industrial Safety and Health Act, xylene is now subject to the "Ordinance on Prevention of Organic Solvent Poisoning" and women cannot work in an environment that contains more than a controlled concentration of 50 ppm.
Use this product to replace xylene.
Examples of comparative staining with xylene and PathoClean (Human)
H&E staining (stomach)
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Left: Xylene 
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Right：PathoClean 
H&E staining (fallopian tube)
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Left: Xylene 
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Right：PathoClean 
Elastica van Gieson staining (lung)
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Left: Xylene 
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Right：PathoClean 
Periodic acid-Schiff staining (kidney)
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Left: Xylene 
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Right：PathoClean 
Masson's trichrome staining (liver)
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Left: Xylene 
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Right：PathoClean 
Data by courtesy of Kasumigaura Medical Center, National Hospital Organization
Examples of comparative staining with xylene and PathoClean (Mouse)
Immunohistochemical staining-PCNA staining
Material: Mouse breast cancer, formalin-fixed
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Left: Xylene 
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Right：PathoClean 
H&E staining
Material: Mouse breast cancer, formalin-fixed
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Left: Xylene 
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Right：PathoClean 
Data by courtesy of Dr. Masaaki Shibata, Department of Anatomy and Cell Biology , Division of Life Sciences, Osaka Medical College
Features
Xylene or other compounds that are compatible with alcohol and paraffin are commonly used as an intermediate agent. PathoClean can be used instead of these compounds in the process of deparaffinization and permeation.
*We recommend PathoMount (Product Number 164-28492, 168-28495) or UI Sol (UI Kasei) as a mounting agent for PathoClean. Please be aware that white turbidity may occur when Marinol (Muto Pure Chemicals Co., Ltd.) is used.
Examples of H&E staining protocol
| Step | Procedure | Time |
|---|---|---|
| 1 | PathoClean (1st) | 5 min. |
| 2 | PathoClean (2nd) | 3 min. |
| 3 | PathoClean (3rd) | 2 min. |
| 4 | PathoClean (4th) | 1 min. |
| 5 | 100% Ethanol (1st) | 1 min. |
| 6 | 100% Ethanol (2nd) | 1 min. |
| 7 | 95% Ethanol | 1 min. |
| 8 | Wash (Water) | 2 min. |
| 9 | Hematoxylin | 6 min. |
| 10 | Wash (Water) | 4 min. |
| 11 | 0.1% Hydrochloric Acid | 1 sec. |
| 12 | Wash (Water) | 5 min. |
| 13 | Eosin | 5 min. |
| 14 | Distilled water | 2 sec. |
| 15 | 100% Ethanol (1st) | 30 sec. |
| 16 | 100% Ethanol (2nd) | 30 sec. |
| 17 | 100% Ethanol (3rd) | 1 min. |
| 18 | 100% Ethanol (4th) | 1 min. |
| 19 | 100% Ethanol (5th) | 1 min. |
| 20 | 100% Ethanol (6th) | 1 min. |
| 21 | PathoClean (1st) | 1 min. |
| 22 | PathoClean (2nd) | 1 min. |
| 23 | PathoClean (3rd) | 1 min. |
| 24 | PathoClean (4th) | 10 sec. |
| 25 | PathoMount (mounting agents) | - |
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.