CYP (Cytochrome P450)

In drug discovery, the effects of compounds on the metabolic reactions specific for various human drug-metabolizing enzymes have been routinely examined. Cytochrome P450 inhibition assay screening test is useful in the investigation of clinical drug-drug interactions (DDI) studies for new drugs

.

CYP Substrate

Testosterone

Testosterone, a well known steroid hormone, is used in Cytochrome P450 (CYP) metabolism inhibition and CYP induction drug interaction studies. The metabolism of testosterone to its hydroxy form, 6β-hydroxytestosterone (6β-OH-T) is a commonly used assay to evaluate human CYP3A enzyme activities.

Reaction in vivo

Conversion of testosterone to 6β-hydroxytestosterone reaction is used to determine enzyme activity of CYP3A4.

00619_img02.png

Example

It is used as a substrate for pharmacokinetics experiments to add to a cell together with a candidate compound that can be a drug and confirm whether it affects drug metabolism.

00619_img03.png

HPLC Charts

  • 00619_img04.png
    teststeron (commercial product)
  • 00619_img05.png
    6β-hydroxytestosterone
  • 00619_img06.png
    purified teststeron (code: 200-20761)
  • HPLC Conditions
    Column Wakopak® Wakosil®-Ⅱ 5C18HG, 4.6mmφ x 250mm
    Temp. 40℃
    Eluent A: water B: acetonitrile
    Flow 1.0 mL/min
    Detector UV 240 nm
  • Eluent Conditions
    Time (min.) A (%) B (%)
    1 - 10 50 50
    10 - 20 50 → 10 50 → 90
    20 - 30 10 90

Product List

  • Open All
  • Close All

CYP Substrate

CYP Inhibitor

CYP Inducer

CYP Metabolite

CYP for Fluorescent Assay

Related Product List

  • Open All
  • Close All

CYP Substrate

Author: A. Samoveanu

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

+49-2131-311-271

Reception hours: Mon-Fri 9:00 - 15:00 (CET)For other hours than the above, please contact us via the inquiry form.