Analysis for PFAS (PFOS, PFOA, PFHxS, etc.)
Perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA), which are polyfluoroalkyl substances (PFCs / PFAS), are difficult to decompose in the environment and have a high accumulation potential. They are subject to various international regulations, including the Stockholm Convention on Persistent Organic Pollutants (POPs)*.
Fujifilm Wako provides pretreatment columns, solvents, and analytical standards that can be used to analyze PFAS (PFOS, PFOA, and PFHxS).
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PFOS (PerFluoroOctaneSulfonic acid) -
PFOA (PerFluoroOctanoic acid) -
PFHxS (PerFluoroHexaneSulfonic acid)
* POPs
Chemical substances that are highly toxic, persistent, bioaccumulative, mobile over long distances, and have adverse effects on human health or the environment are called Persistent Organic Pollutants (POPs). POPs are required to eliminate / limit, reduce emissions, and properly dispose of waste.
3 PFAS Mixture Standard Solution
Fujifilm Wako provides mixture standard solutions and internal standard solution (13C labels) for PFHxS, PFOS, and PFOA which are compounds subject to JP Water Quality Act. Both are mixture standard solutions with clear concentration value of linear PFAS.
Features
✔ Mixture standard solutions with clear concentration value of linear PFAS!
LC/MS Chromatogram (3 PFCs Mixture Standard Solution)
| Peak No. | Constituent | Monitoring Ion (m/z) | Mode |
|---|---|---|---|
| 1 | PFOA | 413 | - |
| 2 | PFHxS | 399 | - |
| 3 | PFOS | 499 | - |
Analysis conditions
[HPLC]
- Column
- ： Wakopak Ultra C18-2 2.1×100 mm
- Delay column
- ： Wakopak Ultra C18-3 2.0×150 mm
- Column temperature
- ： 40℃
- Eluent
- ： A) 0.01 mol/L CH3COONH4 in H2O, B) CH3CN
- Gradient
- ：
Time (min) B conc. (%) 0-2 20 2-22 20-95 22-30 95
- Flow rate
- ： 0.2 mL/min.
[MS]
- Ionization
- ： ESI
- Mode
- ： SIM
Single Analytical Standards
| Structural Formula | Name | CAS | Abbreviation | Carbon Length | Code No. | Volume |
|---|---|---|---|---|---|---|
 |
Perfluorohexanesulfonic Acid Potassium Salt | 355-46-4 3871-99-6 (Potassium Salt) |
PFHxS | 6 | 168-28951 | 100 mg |
 |
1H,1H,2H,2H-Perfluorohexanesulfonic Acid | 757124-72-4 | 4:2 FTS | 6 | 169-29601 | 100 mg |
 |
Perfluoro(2-methyl-3-oxahexanoic) Acid | 13252-13-6 | GenX | - | 166-29591 | 100 mg |
 |
Perfluorooctanoic Acid | 335-67-1 | PFOA | 8 | 161-28941 | 100 mg |
 |
1H,1H,2H,2H-Perfluorooctanesulfonic Acid | 27619-97-2 | 6:2 FTS | 8 | 166-29611 | 100 mg |
 |
N-Methylperfluoro-1-octanesulfonamide | 31506-32-8 | N-MeFOSA | 8 | 138-19521 | 50 mg |
 |
Perfluorodecanoic Acid | 335-76-2 | PFDA | 10 | 167-29381 | 100 mg |
 |
(Z)-2H-Perfluoro-2-decenoic Acid | 161094-76-4 | 8:2 FTUCA | 10 | 161-29661 | 50 mg |
 |
Perfluoroundecanoic Acid | 2058-94-8 | PFUnDA | 11 | 162-29331 | 100 mg |
 |
Perfluorododecanoic Acid | 307-55-1 | PFDoDA | 12 | 169-29341 | 100 mg |
 |
Perfluorotetradecanoic Acid | 376-06-7 | PFTeDA | 14 | 166-29351 | 100 mg |
Solvent for PFAS Analysis
Solvents for PFAS analysis (for PFOS-PFOA Analysis grade) are products that guarantee low levels of PFOA, PFOS, and PFHxS in the solvent.
Features
✔ Guarantee low levels of PFOA, PFOS, and PFHxS in the solvent!
✔ Guarantee the items for HPLC grade (absorbance, peroxides, etc.)!
Presep®-C Agri (Short)
Additive recovery test (PFOA, PFOS) using Presep®-C Agri (Short)
Sample
Ultrapure Water: for PFOS-PFOA Analysis Grade
Standard Solution: PFOS・PFOA mixture standard solution (PFOS (linear): 78 μg/L, PFOA (linear): 100 μg/L)
Internal Standard Solution: 13C-PFOS・13C-PFOA mixture standard solution (PFOS: 100 μg/L, PFOA: 100 μg/L)
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Collection / Concentration Operation
Water (500 mL) + Standard and Internal Standard Solution ▼ Presep®-C Agri (Flow rate 5 mL/min.) ▼ Washing (H2O 5 mL) ▼ Dry (N2 purge) ▼ Extraction (0.1%NH3 in CH3OH 5 mL) ▼ Concentration (→0.5 mL) ▼ LC/MS/MS -
Column Conditioning
- 0.1%NH3 in CH3OH 4 mL
- CH3OH 4 mL
- H2O 4 mL
LC/MS/MS Conditions
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Column: Wakopak® Ultra C18-3 2.0 mm×150 mm
Eluent: A)10 mM CH3COONH4 in H2O, B)CH3CN
LC/MS/MS: Prominence LC-20ADXR / LCMS 8030 plus -
Gradient:
Time (min) B conc. (%) 0-1 25 1-26 25-100 26-30 100 30-30.01 100-25 30.01-35 25
Result
Chromatogram
Recovery Result
| Monitoring Ions (m/z) | Recovery Rate | ||
|---|---|---|---|
| Precursor | Product | ||
| PFOS | 498.8 | 80.0 | 100.9% |
| PFOA | 412.9 | 169.1 | 95.0% |
Product List
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Solid-Phase Extraction Columns
HPLC Column
Delay Column
Solvents
Mixture Standard Solution
Internal Standards Mixture Solution
Analytical Standard
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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