Analysis for PFAS (PFOS, PFOA, PFHxS, etc.)

Perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA), which are polyfluoroalkyl substances (PFCs / PFAS), are difficult to decompose in the environment and have a high accumulation potential. They are subject to various international regulations, including the Stockholm Convention on Persistent Organic Pollutants (POPs)*.

Fujifilm Wako provides pretreatment columns, solvents, and analytical standards that can be used to analyze PFAS (PFOS, PFOA, and PFHxS).

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    PFOS (PerFluoroOctaneSulfonic acid)
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    PFOA (PerFluoroOctanoic acid)
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    PFHxS (PerFluoroHexaneSulfonic acid)

* POPs
Chemical substances that are highly toxic, persistent, bioaccumulative, mobile over long distances, and have adverse effects on human health or the environment are called Persistent Organic Pollutants (POPs). POPs are required to eliminate / limit, reduce emissions, and properly dispose of waste.

3 PFAS Mixture Standard Solution

Fujifilm Wako provides mixture standard solutions and internal standard solution (13C labels) for PFHxS, PFOS, and PFOA which are compounds subject to JP Water Quality Act. Both are mixture standard solutions with clear concentration value of linear PFAS.

Features

✔ Mixture standard solutions with clear concentration value of linear PFAS!

LC/MS Chromatogram (3 PFCs Mixture Standard Solution)

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Peak No. Constituent Monitoring Ion (m/z) Mode
1 PFOA 413 -
2 PFHxS 399 -
3 PFOS 499 -

Analysis conditions

[HPLC]
Column
Wakopak Ultra C18-2 2.1×100 mm
Delay column
Wakopak Ultra C18-3 2.0×150 mm
Column temperature
40℃
Eluent
A) 0.01 mol/L CH3COONH4 in H2O, B) CH3CN
Gradient
Time (min) B conc. (%)
0-2 20
2-22 20-95
22-30 95
Flow rate
0.2 mL/min.
[MS]
Ionization
ESI
Mode
SIM

Single Analytical Standards

Structural Formula Name CAS Abbreviation Carbon Length Code No. Volume
Perfluorohexanesulfonic Acid Potassium Salt 355-46-4
3871-99-6 (Potassium Salt)
PFHxS 6 168-28951 100 mg
1H,1H,2H,2H-Perfluorohexanesulfonic Acid 757124-72-4 4:2 FTS 6 169-29601 100 mg
Perfluoro(2-methyl-3-oxahexanoic) Acid 13252-13-6 GenX - 166-29591 100 mg
Perfluorooctanoic Acid 335-67-1 PFOA 8 161-28941 100 mg
1H,1H,2H,2H-Perfluorooctanesulfonic Acid 27619-97-2 6:2 FTS 8 166-29611 100 mg
N-Methylperfluoro-1-octanesulfonamide 31506-32-8 N-MeFOSA 8 138-19521 50 mg
Perfluorodecanoic Acid 335-76-2 PFDA 10 167-29381 100 mg
(Z)-2H-Perfluoro-2-decenoic Acid 161094-76-4 8:2 FTUCA 10 161-29661 50 mg
Perfluoroundecanoic Acid 2058-94-8 PFUnDA 11 162-29331 100 mg
Perfluorododecanoic Acid 307-55-1 PFDoDA 12 169-29341 100 mg
Perfluorotetradecanoic Acid 376-06-7 PFTeDA 14 166-29351 100 mg

Solvent for PFAS Analysis

Solvents for PFAS analysis (for PFOS-PFOA Analysis grade) are products that guarantee low levels of PFOA, PFOS, and PFHxS in the solvent.

Features

✔ Guarantee low levels of PFOA, PFOS, and PFHxS in the solvent!
✔ Guarantee the items for HPLC grade (absorbance, peroxides, etc.)!

Solvent for PFAS Analysis

Presep®-C Agri (Short)

Additive recovery test (PFOA, PFOS) using Presep®-C Agri (Short)

Sample

Ultrapure Water: for PFOS-PFOA Analysis Grade
Standard Solution: PFOS・PFOA mixture standard solution (PFOS (linear): 78 μg/L, PFOA (linear): 100 μg/L)
Internal Standard Solution: 13C-PFOS・13C-PFOA mixture standard solution (PFOS: 100 μg/L, PFOA: 100 μg/L)

  • Collection / Concentration Operation
    Water (500 mL) + Standard and Internal Standard Solution
    Presep®-C Agri (Flow rate 5 mL/min.)
    Washing (H2O 5 mL)
    Dry (N2 purge)
    Extraction (0.1%NH3 in CH3OH 5 mL)
    Concentration (→0.5 mL)
    LC/MS/MS
  • Column Conditioning
    1. 0.1%NH3 in CH3OH 4 mL
    2. CH3OH 4 mL
    3. H2O 4 mL
LC/MS/MS Conditions
  • Column: Wakopak® Ultra C18-3 2.0 mm×150 mm
    Eluent: A)10 mM CH3COONH4 in H2O, B)CH3CN
    LC/MS/MS: Prominence LC-20ADXR / LCMS 8030 plus

  • Gradient:

    Time (min) B conc. (%)
    0-1 25
    1-26 25-100
    26-30 100
    30-30.01 100-25
    30.01-35 25

Result

Chromatogram
Recovery Result
Monitoring Ions (m/z) Recovery Rate
Precursor Product
PFOS 498.8 80.0 100.9%
PFOA 412.9 169.1 95.0%

Product List

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Solid-Phase Extraction Columns

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HPLC Column

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Delay Column

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Solvents

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*Products that guarantee low levels of PFOA, PFOS, and PFHxS in the solvent.

Mixture Standard Solution

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Internal Standards Mixture Solution

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Analytical Standard

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Author: Y. Nakamura

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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