ScreenFect™ FAQ
Q：Transfection efficiency was low. How should we improve it?
Q：How can we improve the expression level?
Q：Cytotoxicity was high. How should we decrease it?
Q：Which condition for transfection is recommended at the beginning?
Q：Is it necessary to change medium when transfecting?
Q：Is there no problem if medium contains serums or antibiotics?
Q：What are the meanings of 1-Step method and 2-Step method?
Q：Is it possible to transfect multiple genes simultaneously?
Q：How do we use ScreenFect™A and ScreenFect™A plus properly?
Q：Which ScreenFect™ is suitable for the transfection of siRNA?
Q：Which ScreenFect™ is suitable for the transfection of mRNA?
Q：What is SFA P-reagent?
Q：Will you show us the kinds of cells you have achived transfections?
Q：Do you have samples?
Q：What kind of reagent is ScreenFect™?
Q：We preserved ScreenFect™ in a freezer. Isn't there any effect on performance?
Q：How should we adjust plasmid DNA to be used for transfection?
Q：Does serum have any influence on transfection?
Q：Transfection efficiency was low. How should we improve it?
- A
- ： Optimization of the amount of DNA, reagents and other conditions might be able to improve the transfection efficency. Please refer to Optimization Protocol from the following link.
Besides, using both ScreenFect™ and SFA P-reagent (Code No.191-18331) might enable the improvement of the transfection efficiency.
We have free samples for evaluation, so please request from the following Sample Ordering form.
Q：How can we improve the expression level?
- A
- ： Using both ScreenFect™ and SFA P-reagent (Code No.191-18331) might enable the improvement of the expression level.
We have free samples for evaluation, so please request from the following Sample Ordering form.
Q：Cytotoxicity was high. How should we decrease it?
- A
- ： Using both ScreenFect™ and SFA P-reagent (Code No.191-18331) might be able to decrease the cytotoxicity.
We have free samples for evaluation, so please request from the following Sample Ordering form.
Q：Which condition for transfection is recommended at the beginning?
- A
- ： Please refer to the quick protocols for each ScreenFect™ products from the following link.
Q：Is it necessary to change medium when transfecting?
- A
- ： Transfection efficiency and the condition of cells might be improved, if medium is changed to fresh one just before adding transfection reagents. However, it is not always necessary.
Q：Is there no problem if medium contains serums or antibiotics?
- A
- ： Depending on the type of cells, quantity of transfected molecule or other conditions, it is necessary to change the medium. Please consider it according to the purpose of experiment as necessary.
Q：What are the meanings of 1-Step method and 2-Step method?
- A
- ： 1-Step method means Reverse transfection method.
In this method, transfection is done by adding reagent to the cells that are detached just before the transfection.
2-Step method means Forward transfection method.
In this method, transfection is done by adding transfection reagent to the cells that are pre-cultured from the previous day of the transfection.
Q：Is it possible to transfect multiple genes simultaneously?
- A
- ： Yes, it is. Please prepare multiple drug resistant genes.
We recommend the following vector, pEBMulti and pCAG for mammalian expression.
Q：How do we use ScreenFect™A and ScreenFect™A plus properly?
- A
- ： Please use ScreenFect™A plus basically as its transfection efficiency is improved than ScreenFect™A.
When cytotoxicity is high or there is no difference in performance in comparison with ScreenFect™A plus, please consider using a low cytotoxic and inexpensive ScreenFect™A.
Q：Which ScreenFect™ is suitable for the transfection of siRNA?
- A
- ： Please use ScreenFect™siRNA.
ScreenFect™A plus might also transfect siRNA efficiently.
If ScreenFect™siRNA dose not bring about expected results, please consider this as an option.
Q：Which ScreenFect™ is suitable for the transfection of mRNA?
- A
- ： Please use ScreenFect™mRNA.
Using SFA P-reagent together enables transfecting mRNA efficiently and improving expression level.
Depending on the type of cells, using both ScreenFect™A plus and SFA P-reagent can be the best.
If ScreenFect™mRNA does not bring about expected results, please consider this combination as an option.
Q：What is SFA P-reagent?
- A
- ： When transfecting plasmid DNA or mRNA to various types of cells by ScreenFect™, using SFA P-reagent together enables the improvement of transfection efficiency and expression level.
Furthermore, we confirmed that cytotoxicity at transfection is decreased significantly by adding this product.
Q：Will you show us the kinds of cells you have achived transfections?
- A
- ： We have succeeded in transfection to such kinds of cells as general cell lines (HeLa, hepG2, MDCK, MCF-7, K562, etc.), stem cells, hematopoietic cells (macrophage, THP-1, RAW264.7, etc.), microglia, primary cells, insect cells, fish cells and others.
For further details, you can search via Exclusive Website.
Q：Do you have samples?
- A
- ： Yes, we have free samples for evaluation. Please request from the following Sample Ordering form.
Q：What kind of reagent is ScreenFect™?
- A
- ： It is a transfection reagent composed of new cationic liposome.
Q：We preserved ScreenFect™ in a freezer. Isn't there any effect on performance?
- A
- ： No, it isn't. Please do not use it.
Q：How should we adjust plasmid DNA to be used for transfection?
- A
- ： We recommend you to purify plasmid DNA by anion exchange column or cesium chloride density-gradient centrifugation.
Q：Does serum have any influence on transfection?
- A
- ： Although serum has no influence on our Products, we recommend you not to use such anion inhibitors as EDTA or dextran sulfate when transfecting.
In addition, transfection efficiency might be improved if you avoid using antibiotics when transfecting.