ScreenFect(TM)A
- for Genetic Research
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
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0.2mL
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In stock in Japan |
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1mL
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In stock in Japan |
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1mL x5
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In stock in Japan |
Please check here for notes on products and prices.
Document
Overview
ScreenFect™A is a transfection reagent consisting of a new cationic liposome screened*1 by click chemistry. It can be used with various eukaryote-derived cells and can be added directly to mediums containing antibiotics or serum. DNA and siRNA can be transfected into general experimental cell strains (HeLa, HepG2, MDCK, Cos-7, etc.), stem cells (mouse ES cells, etc.), blood cells (macrophages, THP-1, RAW264, 7, etc.), microglia, primary (initial subculture) cells, and Insect cell. Medium replacement after transfection is not required due to low cytotoxicity. The constituent reagents do not contain any poisonous or deleterious substance.
- High transfection efficiency and low cytotoxicity
- Can be used for both DNA and siRNA
- No need to exchange medium and can be used in the presence of serum
*1 Biomaterials. 2012 Nov; 33(32):8160-6. 2012
Outline of ScreenFect™ A protocol
The ideal mixing ratio of DNA/siRNA and transfection reagent varies depending on the type of cells.
We will recommend you to examine several ratios and choose the best one.
DNA transfection
| DNA transfection (/well) | |||||
|---|---|---|---|---|---|
| Plate size | Surface area | Medium volume | Total volume SF-DNA complex |
DNA / Dilution Buffer |
Transfection Reagent / Dilution Buffer |
| 96 wells | 0.3 cm2 | 100 µL | 10 µL | ～100 ng / 5 µL | 0.25 or 0.3 μL / 5 μL |
| 24 wells | 2 cm2 | 500 µL | 50 µL | ～500 ng / 25 µL | 1.25 or 1.5 μL / 25 μL |
| 12 wells | 4 cm2 | 1,000 µL | 100 µL | ～1,000 ng / 50 µL | 2.5 or 3.0 μL / 50 μL |
| 6 wells | 10 cm2 | 2,000 µL | 250 µL | ～2,500 ng / 125 µL | 6.25 or 7.5 μL / 125 μL |
siRNA transfection
| siRNA transfection (/well) | |||||
|---|---|---|---|---|---|
| Plate size | Surface area | Medium volume | Total volume SF-siRNA complex |
siRNA / Dilution Buffer |
Transfection Reagent / Dilution Buffer |
| 96 wells | 0.3 cm2 | 100 µL | 10 µL | 2～3 pmol / 5 μL | 0.1～0.3 μL / 5 μL |
| 24 wells | 2 cm2 | 500 µL | 50 µL | 10～20 pmol / 25 µL | 0.5～1.5 μL / 25 μL |
| 12 wells | 4 cm2 | 1,000 µL | 100 µL | 20～40 pmol / 50 µL | 1.0～3.0 μL / 50 μL |
| 6 wells | 10 cm2 | 2,000 µL | 250 µL | 20～60 pmol / 125 μL | 3.5～7.5 μL / 125 μL |
Data
Comparison with competitor
GFP-expressing plasmid DNA was transfected into HEK293 cells using ScreenFect™ A. The result demonstrated transfection efficiency is equal or superior to competitors. (96-well plate, GFP-expressing plasmid DNA 75 ng/well)
Transfection efficiency of liposome library
GFP-expressing plasmid DNA was transfected into HEK293T cells using a new cationic liposome library synthesized by click chemistry. As a results, a new liposome (ScreenFect™ A) which can transfect plasmid DNA more efficient than company A was confirmed.
Transfection data
- Low cytotoxicity
GFP-expressing plasmid DNA was transfected into HEK293 cells using ScreenFect™ A. The results demonstrated gene transfection efficiency is equal or superior to competitors. Cytotoxicity was also comparable to competitors. (96-well plate, GFP-expressing plasmid DNA 75 ng/well) - Gene transfection into mouse ES cells
GFP-expressing plasmid DNA was transfected into mouse ES cells using ScreenFect™A and GFP-positive cells were detected. As a result, about 60% or more of mouse ES cells were GFP-positive cells.
About DNA transfection
Also applicable to gene transfection into stem cells.
siRNA transfection
LRP6 siRNA was transfected into HEK293 cells using ScreenFect™ A. It showed that a higher knockdown efficiency than the Company A and the Company B. (96-well plate, final concentration of 1 pmole LRP6 siRNA at 2 nM/well)
GAPDH siRNA was transfected into each cell line using ScreenFect™ A. The results demonstrated that a higher knockdown efficiency than competitor. (96-well plate, final concentration of GAPDH siRNA at 3 nM/well)
siRNA transfection
applicable to siRNA transfection.
More Information
List of Cells transfected by ScreenFect™ A or A plus
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References
- Diefenbacher, Markus E., et al. "The LIM Domain Protein nTRIP6 Recruits the Mediator Complex to AP-1-Regulated Promoters." PLoS ONE 9.5 (2014): e97549.
- Freise, Christian, and Uwe Querfeld. "Inhibition of vascular calcification by block of intermediate conductance calcium-activated potassium channels with TRAM-34." Pharmacological Research (2014).
- Hagiwara, Akane, et al. "Luteinizing Hormone-Induced Expression of Ptger4b, a Prostaglandin E2 Receptor Indispensable for Ovulation of the Medaka Oryzias latipes, Is Regulated by a Genomic Mechanism Involving Nuclear Progestin Receptor."
- Peng, Yanyan, Ruidan Xu, and Xiaofeng Zheng. "HSCARG Negatively Regulates the Cellular Antiviral RIG-I Like Receptor Signaling Pathway by Inhibiting TRAF3 Ubiquitination via Recruiting OTUB1." PLoS pathogens 10.4 (2014): e1004041. (3)
- Wakimoto, Hiroaki, et al. "Targetable signaling pathway mutations are associated with malignant phenotype in IDH-mutant gliomas." Clinical Cancer Research (2014). (2)
- Fischer, Simon, et al. "Breaking limitations of complex culture media: Functional non-viral miRNA delivery into pharmaceutical production cell lines." Journal of biotechnology 168.4 (2013): 589-600.
- Bai, Dongmei, et al. "Regulation of the HDM2-p53 pathway by ribosomal protein L6 in response to ribosomal stress." Nucleic acids research 42.3 (2014): 1799-1811.
- Liu, Xing, et al. "Isocitrate dehydrogenase 2 mutation is a frequent event in osteosarcoma detected by a multi‐specific monoclonal antibody MsMab‐1." Cancer medicine 2.6 (2013): 803-814.
Overview / Applications
| Outline | New Liposome developed by click chemistry DNA & siRNA Transfection Reagent Features:
ScreenFect™ A is a transfection reagent composed of new cationic lipids selected by screening experiments *1 by click chemistry. It is applicable to cell lines from various eukaryotic species and can be directly added to medium containing antibiotic and serum. ScreenFect™ A works with DNA and siRNA and can show highly efficient transfection in common cell lines (HeLa, HepG2, MDCK, Cos-7, etc.), stem cells (mouse embryonic stem cells, etc.), blood cells (macrophage, THP-1, RAW264.7, etc.), microglia, primary culture cells. Because of the low cell toxicity, medium change after transfection is not required. Furthermore, this kit does not contain any poisonous and deleterious components. *1: Biomaterials; 2012 Nov; 33 (32): 8160-6. 2012 Kit Contents:
We are regularly updating the application data of ScreenFect™ A. Please contact us about the applicability of your cell strains. This product is for research use only. Do not administer it to human. |
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Property
Manufacturer Information
Alias
- Transfection
ScreenFect？A
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.
The prices are list prices in Japan.Please contact your local distributor for your retail price in your region.