Anti MAP2, Guinea Pig
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
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50uL
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In stock in Japan |
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Product Overview
Microtubule-associated protein 2 (MAP2) is a protein prevalent in neuronal cells. It binds to and stabilizes microtubules1). There are four known isoforms of MAP2: the high molecular weight MAP2a and MAP2b (around 280 kDa), and the low molecular weight MAP2c and MAP2d (around 70 kDa)1). MAP2 is mainly expressed in the cell body and dendrites of neuronal cells and is widely used as a neuronal marker in the field of neuroscience.
The “Anti MAP2, Guinea Pig” is a guinea pig polyclonal antibody, raised against MAP22-11). It can be used to perform multiplex immunohistochemistry.
Antibody Information
| Clonality | Polyclonal |
|---|---|
| Antigen | Rat MAP2 (Full-length) |
| Host | Guinea pig |
| Formulation | Antiserum |
| Conjugate | Unconjugated |
| Cross-reactivity | Mouse , Rat |
| Application | Immunohistochemistry (Frozen Section) 1:500-1,500 |
Protocol (Example)
| 1. Perfusion fixation | Perfuse with sodium citrate/PBS to remove blood, followed by perfusion with 4% PFA. |
|---|---|
| 2. Post-fixation | 4% PFA (24 hours) |
| 3. Immersion in sucrose | 30% Sucrose/PBS (4℃, overnight ~ approximately 2 days) |
| 4. Preparation of frozen sections | After removing sucrose, prepare frozen blocks and cut 30-μm thick sections using a cryostat. |
| 5. Glycine treatment | 25 mM glycine/PBS (approximately 20 minutes) |
| 6. Blocking | 5% normal goat serum in 0.3% Triton X-100/PBS (4℃, overnight) |
| 7. Primary antibody reaction | Anti MAP2, Guinea pig (1:1,500), 1% normal goat serum in 0.3% Triton X-100/PBS (4℃, overnight ~ 2 days). |
| 8. Washing | 0.3% Triton X-100/PBS (5 minutes x 3) |
| 9. Secondary antibody reaction | Alexa Fluor® 488 AffiniPure Goat Anti-Guinea Pig IgG (H+L) (1:500) (Jackson ImmunoResearch, Product Number: 106-545-003), 1% normal goat serum in 0.3% Triton X-100/PBS (room temperature, 2 hours) |
| 10. Washing | 0.3% Triton X-100/PBS (5 minutes x 3) |
| 11. Mounting | Store at 4℃ in a dark place. |
Data
Application Data
Immunohistochemistry
Species: Mouse
Site: Motor cortex
Sample: Frozen section
Antibody concentration: 1:1,500
Data by courtesy of
Dr. Miyata, Department of Applied Biology, Kyoto Institute of Technology
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1:1,500
Data by courtesy of
Dr. Miyata, Department of Applied Biology, Kyoto Institute of Technology
References
- Dehmelt, L. and Halpain, S.: Genome Biol., 6, 204(2005).
The MAP2/Tau family of microtubule-associated proteins - Taniguchi, Y. et al.: Cell Tissue Res., 343, 303(2011).
Localization of plasminogen in mouse hippocampus, cerebral cortex, and hypothalamus - Asai, H., Morita, S. and Miyata, S.: Cell Biochem. Funct., 29(8), 660(2011).
Effect of pleiotrophin on glutamate‐induced neurotoxicity in cultured hippocampal neurons - Mannari, T. et al.: Glia, 61(6), 957(2013).
Astrocytic TRPV1 ion channels detect blood‐borne signals in the sensory circumventricular organs of adult mouse brains - Hourai, A. and Miyata, S.: J. Neurosci. Res., 91(6), 757(2013).
Neurogenesis in the circumventricular organs of adult mouse brains - Morita, S. and Miyata, S.: Cell Biochem. Funct., 31(5), 400(2013).
Synaptic localization of growth‐associated protein 43 in cultured hippocampal neurons during synaptogenesis. - Morita, S. and Miyata, S.: Cell Biochem. Funct., 31(8), 668(2013).
Accessibility of low-molecular-mass molecules to the median eminence and arcuate hypothalamic nucleus of adult mouse - Morita, S. et al.: Cell Tissue Res., 359, 865(2015).
Vascular endothelial growth factor-dependent angiogenesis and dynamic vascular plasticity in the sensory circumventricular organs of adult mouse brain - Furube, E., Morita, M. and Miyata, S.: Cell Tissue Res., 362, 347(2015).
Characterization of neural stem cells and their progeny in the sensory circumventricular organs of adult mouse - Furube, E. et al.: Sci. Rep., 10, 2826(2020).
Neural stem cell phenotype of tanycyte-like ependymal cells in the circumventricular organs and central canal of adult mouse brain - Nambu, Y. et al.: Neurosci. Res., 173, 44(2021).
Effects of leptin on proliferation of astrocyte- and tanycyte-like neural stem cells in the adult mouse medulla oblongata
FAQ
About protocol
- What secondary antibodies can be used?
- At Fujifilm Wako, the following secondary antibodies have been successfully used:
Alexa Fluor® 488 AffiniPure Goat Anti-Guinea Pig IgG (H+L) (Jackson ImmunoResearch, Product Number: 106-545-003)
About application
- What animals can this antibody be used for?
- Fujifilm Wako has confirmed that it can be used for mice and rats. Other species have not been tested.
Overview / Applications
| Outline | MAP2 (Microtubule-associated protein2) is an abundant neuronal cytoskeletal protein. This protein is a well-known neuronal marker. Anti MAP2, Guinea Pig is a guinea pig polyclonal antibody that reacts with MAP2. Antigen: Rat MAP2 Species cross reactivity: Mouse, rat Application: Immunohistochemistry (frozen section) 1:500 - 1:1500 |
|---|---|
| Precautions for Use | Avoid repeated freeze and thaw. |
Property
| Appearance | Liquid |
|---|---|
| Composition | Antiserum |
Manufacturer Information
Alias
- Anti MAP2, Guinea Pig Polyclonal Antibody
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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