Agarose S <Tablet>
- Manufacturer :
- Nippon Gene Co., Ltd.
- Storage Condition :
- Keep at RT.
- Structural Formula
- Label
- Packing
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Comparison
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Product Number
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Package Size
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Price
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Inventory
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0.5G x140
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In stock in Japan |
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0.5G x10
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In stock in Japan |
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Please check here for notes on products and prices.
Document
- Package Insert
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- Spectral Data
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- Certificate of Analysis
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- Calibration Certificate
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- Analytical Charts
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OverView
Feature
- 0.5 g of Agarose S / one tablet
- low EEO
- DNase free, RNase free
- Recommended Gel Concentration: 0.8 - 2 %
- Nucleic Acid Fragment Separation Range: 0.5 - 30 kbp
Application
- Gel Electrophoresis for separation of DNA fragments
- Pulsed-Field Electrophoresis (1 - 2,000 kbp)
- Recovered DNA*1
- Blotting
*1 Recovered DNA from preparative Agarose gels is checked that it can be used for downstream applications (cleavage by restriction endonucleases, ligation by T4 DNA ligase, E. coli transformation by ligated DNA.)
Specification
| Gel strength | ≥ 1,200 g/cm2(1.5 %) |
|---|---|
| Melting point | ≤ 90 °C (1.5 %) |
| Gelling temperature | 37 - 39 °C (1.5 %) |
| Sulfate content (SO4) | ≤ 0.1 % |
| Water content | ≤ 10 % |
| Electroendosmosis (-Mr) | ≤ 0.1 (Low EEO) |
Protocol
Preparation of 1% Agarose S gel
- Add one tablet to 50 mL of TAE or TBE buffer, leave for 5 minutes at room temperature.
- Leave for 5 minutes at room temperature to allow the tablet to fall apart. *1
- Shake gently and heat in a microwave oven or the like after confirming there is no solid material.
Usage Note
Do not heat the solution before the tablets have fallen apart.
When heated in the presence of solid materials with a microwave oven, a gel film is formed on the surface of the solid materials preventing further melting of Agarose, as shown in the bad example below.
Quick Reference Chart of Agarose Tablet
| Number of tablets | 1 tablet | 2 tablets | 3 tablets |
|---|---|---|---|
| Agarose | Buffer volume | ||
| concentration | |||
| 0.80 % | 62.5 mL | 125 mL | 187.5 mL |
| 1.00 % | 50.0 mL | 100 mL | 150 mL |
| 1.20 % | 41.7 mL | 83.3 mL | 125 mL |
| 1.50 % | 33.3 mL | 66.7 mL | 100 mL |
| 2.00 % | 25.0 mL | 50.0 mL | 75.0 mL |
Data
Separation of DNA markers in Agarose S
| Lane | 1 | 2 | 3 | 4 |
|---|---|---|---|---|
| λ Marker | Marker 1 | Marker 2 | Marker 3 | Marker 6 |
| DNA fragments (kbp) |
23.13 | 21.23 | 23.13 | 19.33 |
| 9.42 | 5.15 | 21.23 | 7.74 | |
| 6.56 | 4.98 | 9.42 | 6.22 | |
| 4.36 | 4.27 | 7.42 | 4.26 | |
| 2.32 | 3.52 | 6.56 | 3.47 | |
| 2.02 | 2.02 | 5.81 | 2.69 | |
| 0.56 | 1.91 | 5.65 | 1.88 | |
| 0.13 | 1.58 | 4.88 | 1.49 | |
| 1.38 | 4.36 | 0.93 | ||
| 0.95 | 3.52 | 0.42 | ||
| 0.83 | 2.32 | 0.07 | ||
| 0.56 | 2.02 | |||
| 0.13 | 0.56 | |||
| 0.13 |
Effect of Agarose concentration on the migration distance of bands
Molecular weight markers were electrophoresed using Agarose S 0.5 % gel, 1 % gel and 2 % gel, and the effect of the Agarose gel concentration on the migration distance of the band was investigated. (Use as a reference for determining the concentration when a gel is prepared.)
Ladder Markers
Lane 1：OneSTEP Ladder 1 kb
Lane 2：OneSTEP Ladder 500
Lane 3：OneSTEP Ladder 100
Lane 4：Smart Ladder
Lane 5：OneSTEP Marker 1 (λ/Hind III digest)
Lane 6：OneSTEP Marker 6 (λ/Sty I digest)
Lane 7：OneSTEP Marker 4 (φX174/Hae III digest)
Lane 8：OneSTEP Marker 5 (φX174/Hinc II digest )
| Lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
|---|---|---|---|---|---|---|---|---|
| DNA fragments (kbp) |
10 | 5 | 2 | 10 | 23.1 | 19.3 | 1.35 | 1.057 |
| 9 | 4.5 | 1.8 | 8 | 9.4 | 7.7 | 1.08 | 0.77 | |
| 8 | 4 | 1.6 | 6 | 6.6 | 6.2 | 0.87 | 0.612 | |
| 7 | 3.5 | 1.4 | 5 | 4.4 | 4.3 | 0.6 | 0.495 | |
| 6 | 3 | 1.2 | 4 | 2.3 | 3.5 | 0.31 | 0.392 | |
| 5 | 2.5 | 1 | 3 | 2 | 2.7 | 0.28 | 0.345 | |
| 4 | 2 | 0.9 | 2.5 | 0.6 | 1.9 | 0.27 | 0.341 | |
| 3 | 1.5 | 0.8 | 2 | 0.1 | 1.5 | 0.23 | 0.335 | |
| 2 | 1 | 0.7 | 1.5 | 0.9 | 0.19 | 0.297 | ||
| 1 | 0.5 | 0.6 | 1 | 0.4 | 0.12 | 0.291 | ||
| 0.5 | 0.8 | 0.07 | 0.07 | 0.21 | ||||
| 0.4 | 0.6 | 0.162 | ||||||
| 0.3 | 0.4 | 0.079 | ||||||
| 0.2 | 0.2 | |||||||
| 0.1 |
Recommended Gel Concentration
1 % Agarose S gel (greater than or equal to 1 kb DNA)
2 % Agarose S gel (less than 1 kb DNA)
Overview / Applications
Property
Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.
The prices are list prices in Japan.Please contact your local distributor for your retail price in your region.