MCP-1 (CCL2) ELISA Kits

Monocyte Chemoattractant Protein-1 (MCP-1), also known as CCL2, is a member of CC chemokine family and exhibits chemotactic activity toward monocytes and macrophages. Like many other chemokines, its expression is induced by proinflammatory stimuli such as TNF-α and IL-1. As an inflammatory mediator, MCP-1 is secreted not only by immune cells, such as monocytes and macrophages, but also by a variety of other cell types including vascular endothelial cells, fibroblasts, renal tubular epithelial cells, and smooth muscle cells.
MCP-1 binds to its receptor C-C Chemokine Receptor type 2 (CCR2), enhancing cytokine production by monocytes and macrophages¹⁾ and recruiting these cells to sites of inflammation, thereby prolonging inflammatory responses. Furthermore, MCP-1 released from Th2 cells activates fibroblasts, contributing to the progression of fibrosis, particularly in the liver, lungs, kidneys, and skin²⁾.
MCP-1 plays a central role in the development of insulin resistance by driving inflammation through the infiltration of inflammatory macrophages into the adipose tissue of obese individuals.
MCP-1 is also emerging as a therapeutic target for chronic inflammatory diseases such as rheumatoid arthritis, atherosclerosis, and metabolic syndrome¹⁾.

LBIS™ Human MCP-1 (CCL2) ELISA Kit is a sandwich ELISA kit designed for the quantitative measurement of human MCP-1 (CCL2). This kit enables highly sensitive quantification of MCP-1 (CCL2) in biological samples. The standard included in the kit is a recombinant protein produced in Escherichia coli.

Kit Performance

Assay target	MCP-1 (CCL2)
Calibration curve range	3.85 - 500 pg/mL
Analysis sample	Human Serum/Plasma (EDTA・Heparin)/Urine/ Culture medium (D-MEM・RPMI-1640 etc.)*
Sample volume	20 μL (5x dilution)
Measurement duration	Approx. 3 hours and 50 minutes
Wavelength	Primary wavelength : 450 nm Reference wavelength : 620 nm

• It is tested by using the samples that standard substances have been added to the culture medium.
  Measurement availability depends on the culture medium.
  When using cell culture supernatant as a sample, please make sure to perform recovery test and dilution linearity test on the culture medium before use.

Example of Calibration Curve

Correlation with Other Companies' Products

Human specimens (normal serum, normal plasma [EDTA and heparin]) were measured using both this kit and comparable products from other manufacturers, and the results values were evaluated for correlation.

[Result]
MCP-1 (CCL-2) in normal human serum and plasma was successfully quantified using this kit.
A clear correlation was also confirmed between this kit and assays performed with products from other manufacturers.

LBIS™ Mouse MCP-1 (CCL2) ELISA Kit is a sandwich ELISA kit designed for the quantitative measurement of mouse MCP-1 (CCL2). This kit enables highly sensitive quantification of MCP-1 (CCL2) in biological samples. The standard included in the kit is a recombinant protein produced in Escherichia coli.

Kit Performance

Assay target	MCP-1 (CCL2)
Calibration curve range	3.85 - 500 pg/mL
Analysis sample	Human Serum/Plasma (EDTA・Heparin)/Urine/ Culture medium (D-MEM・RPMI-1640 etc.)*
Sample volume	10 μL (5x dilution)
Measurement duration	Approx. 3 hours and 50 minutes
Wavelength	Primary wavelength : 450 nm Reference wavelength : 620 nm

• It is tested by using the samples that standard substances have been added to the culture medium.
  Measurement availability depends on the culture medium.
  When using cell culture supernatant as a sample, please make sure to perform recovery test and dilution linearity test on the culture medium before use.

Example of Calibration Curve

Correlation with Other Companies' Products

Balb/c mouse samples (normal serum, normal plasma [EDTA and heparin]) were measured using both this kit and comparable products from other manufacturers, and the results values were evaluated for correlation.

[Result]
MCP-1 (CCL-2) in normal mouse serum and plasma was successfully quantified using this kit. A clear correlation was also confirmed between this kit and assays performed with products from other manufacturers.

1. Arefieva I. et al.: Cytokine., 31(6), 439(2005).
   MCP-1-stimulated chemotaxis of monocytic and endothelial cells is dependent on activation of different signaling cascades
2. Hacer S. et al.: Biochimica et Biophysica Acta., 1832(7), 1041(2013).
   Chemokines in tissue fibrosis