VEGF ELISA Kits

Vascular Endothelial Growth Factor (VEGF) is a cytokine specific to vascular endothelial cells, originally discovered in malignant ascites as a factor that enhances vascular permeability. Seven members of the VEGF family are known and are encoded by distinct genes: VEGF-A, VEGF-B, VEGF-C, VEGF-D, the virus-derived VEGF-E, and the placental growth factors PLGF-1 and PLGF-2. Several VEGF members have subtypes arising from differences in splicing patterns.

Among the VEGF family, VEGF-A is involved in the proliferation and survival of vascular endothelial cells and enhanced vascular permeability¹⁾. It exhibits the most potent activity among the VEGF family members. Studies have shown that VEGF-A has subtypes of 121, 165, and 189 amino acid residues arising from alternative splicing. Among these, A165 is the most abundant subtype and is known to promote tumor angiogenesis through a paracrine mechanism. A165 is strongly associated with poor prognosis in several cancers. It is implicated in diseases such as rheumatoid arthritis, arteriosclerosis, and retinopathy.

LBIS™ Human VEGF ELISA Kit is a sandwich ELISA kit for the quantitative measurement of human VEGF. This kit enables highly sensitive quantification of VEGF. The standard included in the kit is a recombinant protein produced in Escherichia coli.

Kit Performance

Assay target	VEGF (VEGF-A165)
Calibration curve range	1.10 - 800 pg/mL
Analysis sample	Human Serum/Plasma (EDTA・Heparin) /Cell culture supernatant*
Sample volume	100 μL
Measurement duration	Approx. 4 hours and 50 minutes
Wavelength	Primary wavelength : 450 nm Reference wavelength : 620 nm

• Measurement availability depends on the culture medium.
  When using cell culture supernatant as a sample, please make sure to perform recovery test and dilution linearity test on the culture medium before use.

Example of Calibration Curve

Measurement of VEGF in Human Serum and Plasma (EDTA/Heparin)

[Result]
This product demonstrated high detection sensitivity and was able to quantitatively detect VEGF at concentrations of several pg/mL.

1. Yoshitomi, T. and Yoshimoto, K.: BUNSEKI KAGAKU, 73(3), 71(2024). (Japanese)
   Nucleic Acid Aptamer for Recognizing Vascular Endothelial Growth Factor (VEGF) Receptors