[NIPPON GENE] Gene Keeper RNA & DNA stabilization solution

• Quickly penetrates the sample and stabilizes RNA and DNA in the tissue/cell
• No need to wash the preservation solution for extraction
• Does not freeze easily at -20°C, and hardly forms crystals
• Ideal for sample transportation in an environment with no liquid nitrogen or dry ice
• No poisonous or deleterious substances included

37℃・・・2 days
25℃・・・2 weeks
4℃・・・1 month
-20℃・・・6 months

1. RNA purification from human buccal cells stored at room temperature in preservation solution

The surface of the oral mucosa was lightly rubbed with a medicine spoon, suspended in PBS, and dispensed into two tubes. After centrifugation to remove the supernatant, each pellet was mixed with Gene Keeper or a competitor's preservation solution, and stored.
After 10 days of storage at room temperature (25℃), RNA was purified using an RNA extraction kit "ISOSPIN Cell & Tissue RNA" (without DNase treatment) (Fig. 1).

Results

The RNA purified from the sample stored in Gene Keeper solution showed distinct bands of 18S rRNA and 28S rRNA. Bands of genomic DNA were also present at the top of the gel.

2. RNA and DNA purification from mouse liver stored at room temperature in preservation solution

Harvested mouse livers were cut and stored at room temperature (25°C) in a ×10 volume of Gene Keeper. The RNA was purified using "ISOSPIN Cell & Tissue RNA," and the DNA was purified using "ISOSPIN Tissue DNA." For comparison, nucleic acids were also purified from a sample frozen in liquid nitrogen immediately after harvesting and stored at -80°C (Control), and from a sample treated with the competitor's product and stored at room temperature, followed by electrophoresis (Figs. 2 and 3).

Results

The RNA and DNA purified from the sample stored in Gene Keeper solution showed equivalent bands to those observed with the competitor's product and the control sample (C).