Protein Extraction Reagents
These reagents are for extracting proteins from animal cells and tissues. Protease inhibitor is not contained.
Cell Lysis Buffer M
This product is a reagent for extracting cytoplasmic proteins from animal cells.
This product is a reagent for extracting cytoplasmic, nuclear, and cell membrane proteins from animal cells. Certain membrane proteins cannot be extracted.
LysoPure™ Nuclear and Cytoplasmic Extractor Kit
This product is a kit for fractionating and extracting cytoplasmic, soluble nuclear, and insoluble nuclear proteins from cultured cells and tissues. After extraction of cytoplasmic fractions and soluble nuclear proteins, insoluble nuclear fraction proteins can be solubilized with SDS Lysis Buffer and extracted as denatured proteins that can be analyzed by Western blotting, etc.
- Cytoplasmic and nuclear proteins can be fractionated and extracted in a stepwise manner.
- The entire process takes only approximately 2 hours.
- The nuclear fraction is less cross-contaminated with cytoplasmic proteins.
- The nuclear fraction can be extracted separately into soluble and insoluble fractions.
Kit composition (for 100 times use)
- Nuclear Fractionation Buffer・・・100 mL
- Nuclear Extraction Buffer・・・75 mL
- SDS Lysis Buffer・・・20 mL
* The number of uses is based on a cell number of 1 x 107/use.
Volume of reagents required for fractionation
Cell number (cells) Nuclear Fraction buffer (μL) Nuclear Extraction buffer (μL) SDS Lysis buffer (μL) 2 x 106 100 50 50 5 x 106 250 125 100 1 x 107 500 250 200
Tissue amount (mg) Nuclear Fraction buffer (μL) Nuclear Extraction buffer (μL) SDS Lysis buffer (μL) 20 100 50 100 50 500 250 200 100 1,000 500 400
Note: In addition, 500 μL each of nuclear fractionation buffer and nuclear extraction buffer are used in the washing process.
Performance comparison-Western blotting
Sample amount at the start
Hela：1 x 107cells
K562：1 x 107cells
Mouse Liver：100 mg
SuperSep™ Ace, 5-20%
SDS-PAGE Buffer, pH8.5
Molecular weight marker
WIDE-VIEW™ Prestained Protein-Size Marker III
Antibodies (primary and secondary)
|Primary antibody(dilution factor)||Secondary antibody(dilution factor)||Molecular Weight (kDa)|
|α-tublin (1:3,000)||Mouse (1:10,000)||50|
|Histon H1 (1:2,000)||32-33|
|Histon H3 (1:2,000)||Rabbit (10,000)||17|
|Lamin B1 (1:3,000)||70|
|COX IV (1:2,000)||17|
- Open All
- Close All
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.