Broad bacteriolytic spectrum

Achromopeptidase®

Achromopeptidase® is a Lysobacter enzymogenes-derived bacteriolytic enzyme. It has a broad bacteriolytic spectrum, is more bacteriolytic than egg-white lysozyme, and lyses a wide range of bacteria. It can also be used to prepare protoplasts such as actinomycetes, etc.

Product Information

Molecular weight: Approximately 20,000

pH stability: Most stable at pH 6.0 and almost stable at pH 5.5 to 10.0

Thermal stability*:

Temperature Activity
40℃ or less Stable
  50℃   95% active
60℃ 75% active
70℃ Almost inactive

* Treated with 50 mmol/L phosphate buffer at pH 6.0 for 10 min

Optimal pH: 7.5 to 8.5

Optimal ionic strength: 10 mmol/L NaCl (in 10 mmol/L Tris-HCl buffer [pH 8.0])

Lytic spectrum

Microorganism Bacteriolytic activity*
TBL-1(300U/mL) Egg-white lysozyme (1,000 U/mL)
Micrococcus caseolyticus +++ -
Micrococcus luteus +++ -
Pediococcus acidilactici +++ +
Staphylococcus aureus +++ ±
Microbacterium arborescens ++ +
Bacillus alvei +++ ±
Clostridium acetobutylicum +++ -
Brevibacterium leucinophagum + ±
Lactobacillus sake +++ +
Enterococcus feacalis +++ ±
Leuconostoc dextranicum ++ ±
Achromobacter liquidum + -
Nocardioides simplex + +
Beijerincka indica + ±
Micobacterium diernhoferi ± ±
Kurthia zopfii ++ +

* Calculated from the decrease in absorbance in each microbial suspension at 600 nm
+++: decreased by ≥80% (within 10 min), ++: decreased by ≥80% (within 60 min), +: decreased by 80%–40% (within 60 min), ±: decreased by 40%–10% (within 60 min), -: decreased by 10%–0% (within 60 min).

Reference

  1. Isono, M., Takahashi, K. and Yamazaki, Y. : Patent Notice, Sho46-42953.
  2. Nakamura, K., Okazawa, Y., Soejima, M. and Masaki, T. : Agric. Biol. Chem., 37, 2667(1973).
  3. Horinouchi, S., Uozumi, T., Beppu, T. and Arima, K. : Agric. Biol. Chem., 41, 2487(1977).
  4. Kotani, S. : Protein, Nucleic Acid, Enzyme, 13, 1136(1968).
  5. Kotani, S. : Protein, Nucleic Acid, Enzyme, 14, 38(1969).

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For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

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