6 × His tag
FUJIFILM Wako supplies antibodies against 6xHis (histidine)-tag can be used to detect 6xHis-tagged fusion proteins. The specificity of the recognition site and application differ depending on the clone.
|Clone No.||Recognition site||Western blot||Immunoprecipitation||ELISA||Labelling||Product Number|
Performance comparison of Western blotting
6xHis-Protein Z: expressed in HEK293T (1 x 105 cells)
Protein P-6xHis: expressed in CHO cells (1 x 105 cells)
Primary Antibody concentration: 1μg/mL
Secondary antibody: anti-mouse IgG antibody, 5,000-fold diluted solution
Detection sensitivity equal or superior to that of Competitive product B was confirmed.
Which clone works best in the detection of N-terminal?
WB：9C11 、IP： 9C11, 28-75
Which clone works best in the detection of C-terminal?
Which clone can recognize the both of N- and C-terminals and is best suited for Western blotting (WB)?
Which clone can recognize the both of N- and C-terminals and is best suited for immunoprecipitation (IP)?
Which clone is versatile and can handle both WB and IP?
Which clone works best with WB?
Which clone works best with IP?
Which is the cheapest clone to recognize the C-terminal alone ?
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Anti 6×His tag, Monoclonal Antibodies
Labeled Anti 6×His tag, Monoclonal Antibodies
Anti 6×His tag, Antibody Beads
Ni-NTA Agarose (Resin)
Ni-NTA Agarose (Cartridge)
Ni-NTA Agarose (Resin, Highly Cross-linked Agarose Beads)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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