Silica Gel Packing Agent (Unmodified)
Silica gel has a strong physical strength and is the most widely used as a column packing agent for analysis and purification in chromatographies such as HPLC, medium and low pressure chromatography, and open column chromatography.
In general, there are unmodified silica gel and chemically modified silica gel that has been changed their functions by binding of various functional groups (C18, NH2 etc.) to the silanol groups on the surface of silica gel.
FUJIFILM Wako provides lineups of crushed silica gel (Wakogel® series) and spherical silica gel (Wakosil® series) as unmodified silica gel. Each series has product lineups with various particle size available for open columns to medium/low pressure chromatography, allowing a smooth scale up from research use to manufacturing process.
How to Select Silica Gel
1. Shape
In general, spherical silica gel is expected to improve resolution and reproducibility compared with crushed silica gel (reasonable price).
FUJIFILM Wako employs crushed silica gel for Wakogel® series and spherical silica gel for Wakosil® series.
2. Chemical Modification
Chemical modification is to bind various functional groups to silanol groups on the surface of silica gel to change silica gel functions. C18 modified silica gel (ODS) is an octadecyl group-bound silica gel and is suitable for reverse-phase chromatography. NH2 modified silica gel is an aminopropyl group-bound silica gel and is suitable for normal-phase, hydrophilic interaction, and ion exchange chromatography. Unmodified silica gel is mainly used for normal-phase chromatography.
3. Particle Size
The larger the particle size, the more suitable for open columns, and the smaller the particle size, the more suitable for flash chromatography or HPLC.
4. Pore Size and Specific Surface Area
The smaller the pore size, the larger the specific surface area of silica gel and the higher resolution of small molecules. On the other hand, the larger the pore size, the higher the resolution of high molecules such as proteins. Suitable pore size must be selected so that sample molecules retain the silica gels well.
Flow Chart for Selection of Silica Gel
Particle Size Distribution Table
The smaller the particle size, the higher the resolution. However, the analysis time becomes longer due to slow flow rate. On the other hand, the larger the particle size, the flow speed becomes faster, and the behavior is suitable for open columns.
List of Physical Properties for each Silica Gel Grade
Silica gel for general purpose (unmodified) | ||||||||
Product Name | Shape | Size | Pore Size | Pore Capacity | Specific Area | pH | Intended Use | Features |
Wakosil® 60 series | ||||||||
Wakosil® 60, 38~63 μm | Spherical | 38~63 μm | 6 nm | - | 450~550 m2/g | 6.2~7.5 | Open column, low pressure, flash chromatography |
This series has a smaller pore size than Wakogel® C series. Therefore, it is suitable for large-scale fractionation and purification owing to its high retention performance. Because of spherical silica gel, it is expected to improve resolution and reproducibility compared with crushed types. Product name: 60 = pore size Shape: Totally porous spherical silica gel with a pore size of 6 nm Use: Open column, low pressure, flash chromatography |
Wakosil® 60, 64 µm~210 μm | 64~210 μm | - | Open column | |||||
Wakogel® 60N series | ||||||||
Wakogel®60N, 38 ~100 µm | Crushed | 38~100 µm | 6 nm | 0.85 mL/g | 550 m2/g | 6.5~7.5 | Open column, low pressure, flash chromatography |
This series has a smaller pore size than Wakogel® C series. Therefore, it is suitable for large-scale fractionation and purification owing to its high retention performance. Product name: 60 = pore size Shape: Crushed silica gel with a pore size of 6 nm Use : Open column, low pressure, flash chromatography |
Wakogel®60N, 63 ~212 µm | 63~212 µm | Open column | ||||||
Wakogel®60N, 150 ~425 µm | 150~425 µm | Open column | ||||||
Wakogel®C series | ||||||||
Wakogel®C-100 | Crushed | 150~425 µm | 7 nm | 0.8 mL/g | 450 m2/g | 5.5~7.0 | Open column | This series is the most common silica gel of FUJIFILM Wako. Product name: The number after C- = mesh size which silica gel passes Shape: Totally porous spherical silica gel Use: Open column, low pressure, flash chromatography |
Wakogel®C-200 | 75~150 µm | 5.5~7.0 | Open column | |||||
Wakogel®C-300 | 45~75 µm | 5.5~7.5 | Open column, low pressure, flash chromatography |
|||||
Wakogel®C series E (economy) type | ||||||||
Wakogel®C-200E | Crushed | 75~150 µm | 7 nm | 0.8 mL/g | 450 m2/g | 5.5~7.5 | Open column | This series has a reasonable price by partially changing the manufacturing process, even though it has the same specifications, including particle size, as Wakogel® C series. Product name: The number after C- = mesh size which silica gel passes Shape: Totally porous spherical silica gel Use: Open column, low pressure, flash chromatography |
Wakogel®C-300E | 45~75 µm | Open column, low pressure, flash chromatography |
||||||
Wakogel®C series HG (high grade) type | ||||||||
Wakogel®C-300HG | Crushed | 40~60 µm | 7 nm | 0.8 mL/g | 450 m2/g | 6.0~7.5 | Open column, low pressure, flash chromatography |
This series is high-grade silica gel with a smaller particle size and a sharp particle size distribution compared with Wakogel® C series. Product name: The number after C- = mesh size which silica gel passes Shape: Totally porous spherical silica gel Use: Open column, medium and low pressure, flash chromatography |
Wakogel®C-400HG | 20~40 µm | Medium and low pressure, flash chromatography |
||||||
Wakogel®C-500HG | 5~20 µm | 6.0~7.0 | Medium and low pressure, flash chromatography |
|||||
Wakogel® FC | ||||||||
Wakogel®FC-40 | Crushed | 20~40 µm | 7 nm | 0.8 mL/g | 450 m2/g | - | Flash chromatography | Silica gel specialized for flash chromatography with a smaller particle size and a sharp particle size distribution. Product name: The number after FC- = particle size Shape: Crushed silica gel Use: Flash chromatography |
Wakosil® HC-N | ||||||||
Wakosil®HC-N | Spherical | 35~63 µm | 3 nm | 0.6 mL/g | 780 m2/g | 6.5~7.5 | Open column, medium pressure, flash chromatography |
This silica gel has larger specific surface area than that of Wakosil® C series. Because of its high retention performance, it has large sample load capacity and high resolution. Shape: Spherical silica gel Use: Open column, medium pressure, flash chromatography. |
For analysis of dioxins | ||||||||
Product Name | Shape | Size | Pore Size | Pore Capacity | Specific Area | pH | Intended Use | Features |
Wakogel® DX | ||||||||
Wakogel® DX | Crushed | 75~150 μm | - | - | - | - | For analysis of dioxins | Silica gel which is guaranteed a low concentration of dioxins by testing ''Suitability for dioxins determination". Shape: Crushed silica gel Use: For analysis of dioxins |
For analysis and separation/purification of petrochemical products | ||||||||
Product Name | Shape | Size | Pore Size | Pore Capacity | Specific Area | pH | Intended Use | Features |
Wakogel® Q series | ||||||||
Wakogel®Q-12 | Crushed | 75~600 µm | - | - | - | 4.0~6.0 | For analysis and separation/purification of petrochemical products | Silica gel for analysis of petrochemical products specific to separation and purification of aromatic hydrocarbons and olefins. Product name: The number after Q- = corresponding Davison's product grade Shape: Crushed silica gel Use: For analysis and separation/purification of petrochemical products |
Wakogel®Q-22 | max. 75 µm | |||||||
Wakogel®Q-23 | 75~150 µm | |||||||
Wakogel®Q-50 | 75~250 µm | |||||||
Wakogel®Q-63 | max. 45 µm |
For analysis of PCB | ||||||||
Product Name | Shape | Size | Pore Size | Pore Capacity | Specific Area | pH | Intended Use | Features |
Wakogel® S-1 | ||||||||
Wakogel®S-1 | Crushed | 63~425 µm | - | - | - | 4.5~6.5 | For analysis of PCB | Silica gel specific to separate PCBs and organochlorine pesticides. Shape: Crushed silica gel Use: For analysis of PCBs and organochlorine pesticides |
Wakosill® 60 Series
Wakosill® 60 series is a spherical silica gel with a pore size of 6 nm that is used for preparative and purification applications. This series has a smaller pore size than Wakogel® C series. Therefore, it is suitable for large-scale fractionation and purification owing to its high retention performance. This is spherical silica gel. Therefore, it is expected to improve resolution and reproducibility compared with crushed types (Wakogel® 60N).
Physical Property Information
Product Name | Shape | Particle Size | Pore Size | Specific Area | pH | Intended Use |
---|---|---|---|---|---|---|
Wakosil® 60, 38 µm~63 μm | Spherical | 38~63 μm | 6 nm | 450~550 m2/g | 6.2~7.5 | Open column, low pressure, flash chromatography |
Wakosil® 60, 64 µm~210 μm | 64~210 μm | Open column |
Wakosill® 60N Series
Wakogel® 60N series is crushed silica gel with a pore size of 6 nm. This series has a smaller pore size than Wakogel® C series. Therefore, it is suitable for large-scale fractionation and purification owing to its high retention performance. This series have 3 types of particle size, available from open column to low pressure and flash chromatography. In addition, Wakogel® 60N, 63 µm~212 µm has a larger retention than equivalent products from other companies, with the chromatographic band also being sharp. (See the examples of use below)
Physical Property Information
Product Name | Shape | Particle Size | Pore Size | Pore Capacity | Specific Area | pH | Intended Use |
---|---|---|---|---|---|---|---|
Wakogel® 60N, 38 µm~100 μm | Crushed | 38~100 μm | 6 nm | 0.85 mL/g | 550 m2/g | 6.5~7.5 | Open column, low pressure, flash chromatography |
Wakogel® 60N, 63 µm~212 μm | 63~212 μm | Open column | |||||
Wakogel® 60N, 150 µm~425 μm | 150~425 μm |
Examples of Use
Separation of azobenzene and p-methoxyazobenzene
Wakogel® 60N: Larger retention and better separation than equivalent products from other company
Wakogel® C-200: Suitable for preparative purification in a short time with rapid elution
-
Fractionation Condition
Column φ15 mm x 300 mm (filled to a height of 150 mm: approx. 13 g) Eluent Toluene/n-Hexane = 2/1 (v/v) Detection UV 330 nm Sample ①Azobenzene 1 mg/mL ②p-Methoxyazobenzene 1 mg/mL Sample load 0.5 mL -
HPLC Condition
Column Wakopak® Wakosil® 5SIL-120 4.6 mm x 250 mm Eluent Toluene/n-Hexane = 1/1 (v/v) Injection vol. 10 μL Flow rate 1.0 mL/min. at 40℃ Detection UV 330 nm
Procedure
- Fractions were collected under fractionation conditions.
- Each fraction was analyzed by HPLC, and the obtained peak values were traced to the elution volume under the fractionation conditions.
Wakogel® C Series
Wakogel® C Series
Wakogel® C series is totally porous crushed silica gel with a pore size of 7 nm and is the most common silica gel of FUJIFILM Wako’s products. This series have 3 types of particle size, available from open column to low pressure and flash chromatography.
Product Name | Shape | Particle Size | Pore Size | Pore Capacity | Specific Area | pH | Intended Use |
---|---|---|---|---|---|---|---|
Wakogel® C-100 | Crushed | 150~425 μm | 7 nm | 0.8 mL/g | 450 m2/g | 5.5~7.0 | Open column |
Wakogel® C-200 | 75~150 μm | 5.5~7.0 | |||||
Wakogel® C-300 | 45~75 μm | 5.5~7.5 | Open column, low pressure, flash chromatography |
Wakogel® C Series E (economy) Type
Wakogel® C series E type is silica gel that achieves a reasonable price by partially changing the manufacturing process, even though it has the same specifications, including particle size, as Wakogel® C series.
Product Name | Shape | Particle Size | Pore Size | Pore Capacity | Specific Area | pH | Intended Use |
---|---|---|---|---|---|---|---|
Wakogel® C-200E | Crushed | 75~150 µm | 7 nm | 0.8 mL/g | 450 m2/g | 5.5~7.5 | Open column |
Wakogel® C-300E | 45~75 μm | Open column, low pressure, flash chromatography |
Wakogel® C Series HG (high grade) Type
Wakogel® C series HG type is high-grade silica gel with a smaller particle size and a sharp particle size distribution compared with Wakogel® C series. In addition, it has the fine powder removed as much as possible by an increased classification precision, and it can be used at a higher flow rate than similar particle sizes products.
Product Name | Shape | Particle Size | Pore Size | Pore Capacity | Specific Area | pH | Intended Use |
---|---|---|---|---|---|---|---|
Wakogel® C-300HG | Crushed | 40~60 μm | 7 nm | 0.8 mL/g | 450 m2/g | 6.0~7.5 | Open column, low pressure, flash chromatography |
Wakogel® C-400HG | 20~40 μm | Medium and low pressure, flash chromatography | |||||
Wakogel® C-500HG | 5~20 μm | 6.0~7.0 | Medium and low pressure, flash chromatography |
Separation and Purification of Saikosaponin Using Wakogel®
HPLC chromatogram in each purification step
Wakogel® FC Series
Wakogel® FC series is silica gel specialized for flash chromatography with a smaller particle size and a sharp particle size distribution.
Physical Property Information
Product Name | Shape | Particle Size | Pore Size | Pore Capacity | Specific Area | Intended Use |
---|---|---|---|---|---|---|
Wakogel® FC-40 | Crushed | 20~40 μm | 7 nm | 0.8 mL/g | 450 m2/g | Flash chromatography |
Wakosil® HC-N
Wakosil® HC-N has larger specific surface area than that of Wakosil® C series. Because of its high retention performance, it has large sample load capacity and high resolution.
Physical Property Information
Product Name | Shape | Particle Size | Pore Size | Pore Capacity | Specific Area | Loss on drying | pH | Intended Use |
---|---|---|---|---|---|---|---|---|
Wakosil® HC-N | Spherical | 35~63 μm | 3 nm | 0.6 mL/g | 780 m2/g | max. 15% | 6.5~7.5 | Open column, medium pressure, flash chromatography |
Wakogel® Q Series
Wakogel® Q Series for analysis of petrochemical products specific to separation and purification of aromatic hydrocarbons and olefins. The number after Q- = corresponding Davison’s product grade.
Physical Property Information
Product Name | Shape | Particle Size | pH | Intended Use |
---|---|---|---|---|
Wakogel® Q-12 | Crushed | 75~600 μm | 4.0~6.0 | Silica gel increasing the adsorption capacity of aromatic hydrocarbons. Separation of aromatic hydrocarbons in petrochemical and coal tar industries. |
Wakogel® Q-22 | 75 μm or less | Separation of aromatic hydrocarbons and olefins by type. | ||
Wakogel® Q-23 | 75 ~150 μm | Analysis of liquid saturated hydrocarbons and aromatic hydrocarbons. | ||
Wakogel® Q-50 | 75~250 μm | Analysis of liquid saturated hydrocarbons, olefins and aromatic hydrocarbons with the FIA (Flow Injection Analysis) method. | ||
Wakogel® Q-63 | 45 μm or less | Equivalent product to Davison Grade 63. |
Wakogel® DX, Wakogel® S-1
Wakogel® DX is guaranteed a low concentration of dioxins by testing ‘‘Suitability for dioxins determination”.
Wakogel® S-1 specific to separate PCBs and organochlorine pesticides.
Physical Property Information
Product Name | Shape | Particle Size | pH | Intended Use |
---|---|---|---|---|
Wakogel® DX | Crushed | 75~150 μm | - | For analysis of dioxins |
Wakogel® S-1 | 63~ 425 μm | 4.5~6.5 | For analysis of PCBs |
Product List
- Open All
- Close All
Wakogel® 60N
Wakogel® C Series
Wakogel® LP Series
Wakogel® FC Series
Wakogel® Q Series
Wakogel® DX
Wakogel® S-1
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.
The prices are list prices in Japan.Please contact your local distributor for your retail price in your region.