CYP (Cytochrome P450)
Testosterone, a well known steroid hormone, is used in Cytochrome P450 (CYP) metabolism inhibition and CYP induction drug interaction studies. The metabolism of testosterone to its hydroxy form, 6β-hydroxytestosterone (6β-OH-T) is a commonly used assay to evaluate human CYP3A enzyme activities.
Reaction in vivo
Conversion of testosterone to 6β-hydroxytestosterone reaction is used to determine enzyme activity of CYP3A4.
It is used as a substrate for pharmacokinetics experiments to add to a cell together with a candidate compound that can be a drug and confirm whether it affects drug metabolism.
Column Wakopak® Wakosil®-Ⅱ 5C18HG, 4.6mmφ x 250mm Temp. 40℃ Eluent A: water B: acetonitrile Flow 1.0 mL/min Detector UV 240 nm
Time (min.) A (%) B (%) 1 - 10 50 50 10 - 20 50 → 10 50 → 90 20 - 30 10 90
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CYP for Fluorescent Assay
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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