Trypsin-EDTA Solution (without Phenol Red), AF

This product is a trypsin EDTA solution that has been tested for mycoplasma, endotoxin, and sterility. It can be used to detach adherent cells and disperse cells in various tissues.

As this product is an animal component-free product made from recombinant trypsin, it can be safely used in experiments without viral contamination.

Features

  • Animal-derived component-free
  • Efficiently detaches cells with good survival after detachment

Product Specifications

Specification item
Appearance
pH
Osmotic pressure
Sterility test
Endotoxin test
Mycoplasma test
Practical test
(Vero cells are completely detached after 10 minutes, and the viable cell rate is ≥90%)

Ability to detach cells


Cell: MDCK cell
Medium: E-MEM + 10% FBS
Number of seeded cells: 5.0×104 cells/cm2
Culture method: Static culture
Culture equipment: 12-well plate
Culture environment: 37℃,5%CO2, 4 days
After cells were cultured and then treated at 37℃, 5% CO2 for 15 minutes, the number of detached cells was counted.
→ Detached the cells more efficiently than the competing product

Effects during subculture

<Culture conditions>
Cell: MDCK cell
Medium:E-MEM+10%FBS
Number of seeded cells:3.0×105 cells
Culture method: Static culture
Culture equipment: T25 flask
Culture environment:37℃,5%CO2

Cells were treated with trypsin at 37℃,5%CO2 for 15 minutes and then subcultured.
→ It was shown that this product had no negative effect on cell proliferation.

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Trypsin-EDTA (Uses Procine Parvovirus-tested Ingredients)

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

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