Wako Blog

We introduce a wide range of topics of research, technical reports, tips on experiments and so on.

Talking of LAL

Water for Bacterial Endotoxin Test

This article was written by Dr. Masakazu Tsuchiya, FUJIFILM Wako Pure Chemical Corporation, for Vol. 62, No. 1 (July 1994) of Wako Junyaku Jiho.
The content of this article is from the time of publication. It is not the latest information due to new knowledge and changes in regulatory rules after original publication.

The Limulus Amebocyte Lysate (LAL) test is a technique for detecting endotoxins that uses a reagent formulated from the blood cells of the Limulus horseshoe crab. Water for the Bacterial Endotoxin Tes...

Talking of LAL

Horseshoe Crab

This article was written by Dr. Masakazu Tsuchiya, FUJIFILM Wako Pure Chemical Corporation, for Vol. 61, No. 4 (July 1993) of Wako Junyaku Jiho.
The content of this article is from the time of publication. It is not the latest information due to new knowledge and changes in regulatory rules after original publication.

Called "living fossils," the horseshoe crab has inhabited the earth's oceans for about 200 million years with little change in morphology. As with spiders and scorpions, it is said that these crabs ar...

Talking of LAL

β-glucan

This article was written by Dr. Masakazu Tsuchiya, FUJIFILM Wako Pure Chemical Corporation, for Vol. 61, No. 2 (April 1993) / Vol. 61, No. 3 (July 1993) of Wako Junyaku Jiho.
The content of this article is from the time of publication. It is not the latest information due to new knowledge and changes in regulatory rules after original publication.

LAL reacts not only to endotoxin, but also to (1→3)- β-D-glucan to form a gel. This phenomenon was reported by two Japanese groups in 1981. The first, Dr. Kakinuma from Takeda Pharmaceutical Company, ...

Talking of LAL

Drug Effects

This article was written by Dr. Masakazu Tsuchiya, FUJIFILM Wako Pure Chemical Corporation, for Vol. 60, No. 3 (July 1992) / Vol. 60, No. 4 (October 1992) / Vol. 61, No. 1(January 1993) of Wako Junyaku Jiho.
The content of this article is from the time of publication. It is not the latest information due to new knowledge and changes in regulatory rules after original publication.

Endotoxin measurements of drug products should be carried out under conditions in which the drug does not interfere with the measurement. Here we will look at some methods used to establish conditions...

Talking of LAL

Maximum Valid Dilution

This article was written by Dr. Masakazu Tsuchiya, FUJIFILM Wako Pure Chemical Corporation, for Vol. 60, No. 2 (April 1992) of Wake Junyaku Jiho.
The content of this article is from the time of publication. It is not the latest information due to new knowledge and changes in regulatory rules after original publication.

When using the Limulus test to measure the concentration of endotoxin in a drug product, the product normally contains factors which could interfere with the assay. Dilution is the most common method ...

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