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Mature BDNF ELISA Kit Wako, High Sensitive

for Immunochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
GHS :
  • Structural Formula
  • Label
  • Packing
SDS
Comparison
Product Number
Package Size
Price
Inventory
Distributor
298-83901
Barcode No
4548995085146
96Tests
List Price
650.00 USD

In stock

Document

SDS
Product Specification Sheet
Spectral Data
Certificate of Analysis
Calibration Certificate

Kit component

Kit Component

Antibody-coated Plate 1 plate
Mature BDNF Standard 1 bottle
Buffer 60 mL
Biotin-conjugated Antibody Solution 100 μL
Peroxidase-conjugated Streptavidin Solution 100 μL
Luminescent Reagent 1 6 mL
Luminescent Reagent 2 6 mL
Wash Solution (10 x) 100 mL
Plate Seal 4 sheets

Features

Standard Curve

04041888_img01.png
  • Detects mBDNF level in mouse plasma/serum
  • Detects mBDNF specifically

Performance

  • Standard curve range: 0.205~50 pg/mL
  • Specificity: mBDNF*1
  • Sample: Mouse serum and plasma / Rat serum and plasma*2
  • Sample volume: 13 μL (4 times dilution)
  • Assay time: 4 hours
  • Detection method: Luminescent method*3

*1: 0.5% reaction to human proBDNF
*2: antibody reacts with human, mouse and rat BDNF
*3: requires a microplate reader for luminescence measurement

Comparison with Mature BDNF ELISA Kit Wako (Code No. 296-83201)

Products Mature BDNF ELISA Kit Wako, High Sensitive Mature BDNF ELISA Kit Wako
Code No. 298-83901 296-83201
Standard curve range 0.205 - 50 pg/mL 4.1 - 1,000 pg/mL
Specificity mature BDNF mature BDNF
Cross-reactivity to human proBDNF <0.5% Apporox. 10%
Sample Mouse serum and plasma Rat serum and plasma Human serum and plasma
Sample volume 13 μL (4 times dilution) Serum: 10μL (10 times dilution)
Plasma: 5μL (20 times dilution)
Assay time 4 hours 4 hours
Detection method Luminescent method Colorimetric method

Assay principle

This kit is a sandwich ELISA using mBDNF monoclonal antibody and mBDNF polyclonal antibody. The kit has low cross-reactivity to proBDNF (approx. 10%) because the monoclonal antibody reacts with N-terminal cutting surface of mBDNF.

04041888_img02.png
FUJIFILM Wako Company A Company B Company C
Cross-reactivity to proBDNF <0.5% Approx. 10% Approx. 15% Approx. 50%
Sensitivity
(Lowest point of Standard curve)
0.2 pg/mL 62.5 pg/mL 15.6 pg/mL 15.0 pg/mL

Data

Spiked recovery test

  • Sample Spike mBDNF
    (pg/mL)
    Assay value
    (pg/mL)
    Recovery volume
    (pg/mL)
    Recovery
    (%)
    Mouse
    plasma①
    0.0 0.622
    1.00 1.57 0.948 94.8
    10.0 10.5 9.88 98.8
    30.0 31.0 30.4 101
    Mouse
    plasma②
    0.0 1.22
    0.50 1.77 0.550 110
    1.00 2.13 0.910 91.0
    10.0 11.5 10.3 103
    20.0 20.7 19.5 97.5
    Mouse
    Serum①
    0.0 <0.205
    1.0 0.959 0.959 95.9
    10.0 10.0 10.0 100
    30.0 30.2 30.2 101
    Mouse
    Serum②
    0.0 0.82
    0.50 1.32 0.500 100
    1.00 1.76 0.940 94.0
    10.0 10.5 9.68 96.8
    20.0 19.5 18.7 93.5
  • Sample Spike mBDNF
    (pg/mL)
    Assay value
    (pg/mL)
    Recovery volume
    (pg/mL)
    Recovery
    (%)
    Rat
    pool plasma
    0.0 <0.205
    1.0 1.06 1.06 106
    5.0 4.80 4.80 96.0
    10.0 10.5 10.5 105
    20.0 20.9 20.9 105
    Rat
    pool serum
    0.0 1.08
    1.0 2.13 1.05 105
    5.0 5.87 4.79 95.8
    10.0 10.6 9.52 95.2
    20.0 20.6 19.50 97.5

Dilution linearity

  • Mouse plasma

    04041888_img03.png
  • Mouse serum

    04041888_img04.png

Example of measurement: Autism model mouse sample

Mouse serum/plasma mBDNF levels were measured by this kit. The all samples were able to detect mBDNF level.

Sample Mouse Sample No. Measured (pg/mL)
Serum BTBR mouse*4
(Autism model mouse)
BTBR-1 12.4
BTBR-2 18.1
BTBR-3 16.1
C57BL/6J
(Wild type mouse)
B6J-1 6.96
B6J-2 9.80
B6J-3 9.01
Plasma (heparin) BTBR mouse*4
(Autism model mouse)
BTBR-4 11.1
BTBR-5 9.73
C57BL/6J
(Wild type mouse)
B6J-4 6.93
B6J-5 11.6

*4: BTBR T + Itpr3tf/J mouse

Overview / Applications

Outline This item is designed for the quantitative detection and measurement of mature BDNF in biological fluids such as serum, and plasma. The product can measure low level of mBDNF in mouse blood by using a luminescence detection system.

Brain-derived neurotrophic factor(BDNF) is a member of NGF family of neurotrophic factors. it is known that BDNF is involved in neurogenesis and synaptogenesis. Therefore BDNF is expected as biomarker for diseases of nervous system.In addition, it has been reported to be related to heart disease, diabetes, gout, periodontal disease, stress, exercise, etc., and it is the target of research in a wide range of fields.BDNF has precursor termed proBDNF, which is converted to mature BDNF(mBDNF) through the proteolytic removal of the N-terminal fragment by specific protease. It is reported that there are different functions between mBDNF and proBDNF.

[Standard curve] 0.2-50pg/mL
[Sample] mouse plasma and serum
[Sample volume] 13uL
[Assay time] 4 hour
[Detection method] luminescent method

Property

Manufacturer Information

Alias

  • mBDNF ELISA Kit

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