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MagCapture™ Exosome Isolation Kit PS

for Genetic Research
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
  • Structural Formula
  • Label
  • Packing
SDS
Comparison
Product Number
Package Size
Price
Inventory
Distributor
299-77603
Barcode No
4548995061461
2Tests
List Price
220.00 USD

In stock

Distributor
293-77601
Barcode No
4548995050786
10Tests
List Price
595.00 USD

In stock in Japan

Document

SDS
Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Preprocessing protocols

When exosomes and other large EVs (microvesicles) are needed, prepare 1,200 × g supernatant※1 as a sample. Additionally, when highly purified exosomes are needed, prepare 10,000 × g supernatant※2 as a sample. This protocol for sample preparation is set for cell culture medium, serum, and plasma. When other body fluids are used, please examine the appropriate preprocessing protocol by referring to the protocol for serum and plasma.

Cell Culture Medium

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Serum and Heparin Plasma

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EDTA Plasma

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Protocol for buffer exchange (ultrafiltration)

Perform buffer exchange of 1 mL centrifuged EDTA plasma sample with 50 mL of TBS buffer.

  1. Add 19 mL of TBS to Vivaspin20 (100K).
  2. Add the 1 mL of centrifuged EDTA plasma sup. to the Vivaspin20 (100K) of 1. and mix (solution A).
  3. Centrifuge solution A at 4°C.  (Refer to centrifugal condition in the manufacturer’s instruction manual.)
  4. Add 10 mL of TBS to solution A when the upper liquid volume is dropped (solution B).
  5. Centrifuge solution B at 4°C.
  6. Add 10 mL of TBS to solution B when the upper liquid volume is dropped (solution C).
  7. Centrifuge solution C at 4°C.
  8. Add 11 mL of TBS to solution C when the upper liquid volume is dropped.
  9. Centrifuge solution C at 4°C until the volume becomes under 1 mL.
  10. Proceed to “Isolation step”.

※1 When exosomes and large EVs are needed, use 1,200 × g sup. fraction as sample.
※2 When exosomes are needed, use 10,000 × g sup. fraction as sample.
※3 When Large EVs are needed, use ppt of Large EVs obtained by centrifugation at 10,000 x g as sample after suspending it with TBS.
※4 The concentration step is an option when using a large volume (~ 50 mL) of cell culture supernatant as a sample for purification. However, since recovery efficiency improves, please perform it as much as possible.

Note : a volume of large or small sample
■In the case of large volume
The concentration of the sample is recommended when using a large volume (~50 mL) of cell culture supernatant as a sample for purification. Since recovery efficiency improves, please perform it as much as possible.

■In the case of small volume
Add the appropriate volume of TBS into samples to reach the volume of 0.5 mL to obtain the better mixture of the Exosome Capture-immobilized beads with the sample. (Example: 100-200 μL → 500 μL)

Outline of Procedure

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User's Voice

"This kit was the most successful exosome isolation method I have ever tried."

Biopharmaceutical company | Sample: Mesenchymal stem cells

"Because of the low back, the glycan analysis was successful. This kit provided the basis for determining exosomes as one of the theme in our laboratory."

University-Medical school | Sample: Human urine

"We have discovered a disease marker protein from serum-derived exosomes isolated with this kit."

University-Medical school | Sample: Human serum

"Exosomes are very pure with this kit compared to other methods of isolating exosomes depending on a sample."

Cancer research organization | Sample: Human plasma / serum

"Analysis of the exosomes isolated with this kit revealed candidate proteins for disease markers."

University-Medical school | Sample: Human urine

Overview / Applications

Outline This product is for research use only. Do not administer it to human.
This kit can conveniently obtain high-purity exosomes from the cell culture supernatant, serum and plasma etc samples by affinity method. Exosomes can be eluted in an intact state with a chelating agent because it is applied the substances that calcium-dependent binding to phosphatidylserine (PS) present on the membrane surface of exosomes.
Features
1. Simple isolation of extracellular vesicles by novel affinity method for phosphatidylserine
2. High efficiency and high purity exosome can be obtained than conventional ultracentrifugation method
3. Intact extracellular vesicles can be obtained by elution under neutral conditions with a chelating agent ,and it can be used for various applications
4. Simple operation with magnetic beads
[Features]
1) Novel affinity purification method is adopted.
· Recovery by a PS-affinity molecule
· Low background
· Mild elution by a chelating reagent at a neutrality condition
2)Require no ultracentrifugation
· Improved operability by using magnetic beads
· Optimized protocol
Kit Components Kit Contents:
10 purification
1) Streptavidin Magnetic Beads 600 uL x 1 tube
2) Biotin-labeled Exosome Capture 100 uL x 1 tube
3) Exosome Capture Immobilizing Buffer 35 mL x 1 bottle
4) Exosome Binding Enhancer (x 500) 500 uL x 1 tube
5) Washing Buffer 75 mL x 2 bottles
6) Exosome Elution Buffer 5 mL x 1 bottle
7) Reaction Tubes 22 tubes

2 purification
1) Streptavidin Magnetic Beads 120 uL x 1 tube
2) Biotin-labeled Exosome Capture 20 uL x 1 tube
3) Exosome Capture Immobilizing Buffer 7 mL x 1 bottle
4) Exosome Binding Enhancer (x 500) 100 uL x 1 tube
5) Washing Buffer 30 mL x 1 bottles
6) Exosome Elution Buffer 1 mL x 1 bottle
7) Reaction Tubes 4 tubes

Property

Manufacturer Information

Alias

  • Exosome Isolation Kit PS

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

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