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Anti Iba1, Goat

for Immunochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.
  • Structural Formula
  • Label
  • Packing
SDS
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Product Number
Package Size
Price
Inventory
Distributor
011-27991
Barcode No
4548995076397
100uL
List Price
450.00 USD

In stock

Document

SDS
Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

50% OFF Promotion

Promotion Overview

Product code 011-27991
Product name Anti Iba1, Goat
Package Size 100uL
Promotion price 450.00 USD225.00 USD 50%OFF
Promotion period 1st March, 2021 ~ 30th April, 2021

Conditions

  • Discount will apply to orders placed through our online store.
  • This promotion does not apply to any distributors or resellers.
  • When you purchase it on EC, it will be displayed as 450.00 USD, but the actual invoice/packing list will show the discounted price.
  • This promotion is open to customers in the US.
  • Limit 5 vials per customer.
  • Discount will apply to orders received by FUJIFILM Wako Chemicals USA no later than 30th April 2021, or until promotional supplies are depleted, whichever comes first.

Don't miss the oppotunity!

Antibody Information

Application Immunohistochemistry (frozen section) 1:200-1,000
Immunohistochemistry (paraffin section) 1:200-1,000
Western Blotting 1:1,000
Species reactivity Mouse, Rat Clonality Polyclonal
Immunogen Synthetic peptide
C-terminal of Iba1
Concentration 0.5-0.7 mg/mL Label Unconjugated
Antigen synonyms Allograft inflammatory factor 1, AIF1, IRT1 Host Goat
Preparation Purified by the antigen affinity chromatography from goat antisera and prepared in TBS solution. Contains no preservatives and stabilizers.
Background Iba1 is a macrophage- and microglial-specific calcium binding protein which molecular weight is 17,000, widely used as a marker for microglia.

Application Data

Immunohistochemistry (fluorescent staining)

03700482_img04.png

Immunohistochemistry of frozen section in mouse model of Alzheimer’s disease (APPNL-G-F mouse) brain cerebral neocortex using anti Iba1, goat polyclonal antibody at dilution of 1:1,000, and anti goat IgG, Alexa Flour 488-conjugated. Aβ were stained by 0.001% FSB solution(Amyloid fluorescent probe). The data were provided by Dr. Sakakibara, National Center for Geriatrics and Gerontology in Japan.

03700482_img07.png

Immunohistochemistry of wild type mouse brain hippocampus using anti Iba1, goat polyclonal antibody(1:200), and anti goat IgG, Alexa Flour 488-conjugated. Nuclear were stained by Hoechst33342.
The data were provided by Dr. Takata, Kyoto Pharmaceutical University in Japan.

 

03700482_img01.png

Immunohistochemistry of frozen section in rat(left) and mouse(right) brain cerebral cortex using anti Iba1, goat polyclonal antibody at dilution of 1:250, and anti goat IgG, Alexa Flour 488-conjugated.
The data were provided by Dr. Nakajima from Soka University in Japan.

 

Immunohistochemistry(DAB staining)

03700482_omg02.png

Sample: paraffin section of mouse brain frontal lobe
1st antibody: Anti Iba1, goat(1:1.000)
2nd antibody: Anti goat IgG, biotin-conjugated
Antigen retrieval: 10mM citric acid buffer(pH6.0), 90℃, 10min

 

Western Blotting

03700482_omg03.png

The data were provided by Dr. Nakajima from Soka University in Japan.

Sample:
Microglia from rat primary culture 10μg
Neuron from rat primary culture 10μg
Astrocyte from rat primary culture 10μg
Cerebral cortex from rat brain 100μg

1st antibody: Anti Iba1, goat(1:1.000)
2nd antibody: Anti goat IgG, HRP-conjugated

Overview / Applications

Outline Iba1 is a calcium-binding protein with a molecular weight of 17,000 specifically expressed in macrophage and microglia.This product is goat polyclonal antibody that specifically recognize Iba1, and is available for a microglial marker.

Antigen: synthetic peptide(C-terminal of Iba1)
Cross-reactivity: mouse, rat
Applications:
Immunohistochemistry(frozen section) 1:200-1,000
Immunohistochemistry(paraffin section) 1:200-1,000
Western Blotting 1:1,000

References
1)Karen, M. R., et al.: J. Neuroimmunol.,338, 577082(2020).
Dexamethasone attenuates inflammatory-mediated suppression of B2-adrenoceptor expression in rat primary mixed glia
https://pubmed.ncbi.nlm.nih.gov/31707103/
ICC, rat cell

2)
Saini, Y., et al.: Cytotechnology, 4, 589(2020).
IL-4 and IL-10 promotes phagocytic activity of microglia by up-regulation of TREM2
https://pubmed.ncbi.nlm.nih.gov/32623621/
ICC, mouse cell

3)
Orli, T. Z., et al.: Theranostic, 11, 1, 346(2021).
A new ketogenic formulation improves functional outcome and reduces tissue loss following traumatic brain injury in adult mice
https://pubmed.ncbi.nlm.nih.gov/33391479/
IHC, mouse brain
Cat. No. was wrong in this paper

4)
Kim, J., et al.: Front. Cell. Neurosci., 15, 638098 (2021).
Key Role of Microglial Matrix Metalloproteinases in Choroidal Neovascularization
https://pubmed.ncbi.nlm.nih.gov/33716674/
IHC, mouse eye

5)
Garima, S., et al.: J. Neuroinflammation, 18, 1, 69(2021).
Fetal inflammation induces acute immune tolerance in the neonatal rat hippocampus
https://pubmed.ncbi.nlm.nih.gov/33706765/
IHC, rat brain

Property

Appearance Liquid

Manufacturer Information

Alias

  • Anti Iba1, Goat Polyclonal Antibody
    Anti AIF1, Goat Polyclonal Antibody
    Anti IRT1, Goat Polyclonal Antibody

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

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