LabAssay (TM) Creatinine
- Manufacturer :
- FUJIFILM Wako Shibayagi Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
- GHS :
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- Structural Formula
- Label
- Packing
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500Tests
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Document
Kit component
Kit component
| Deproteinizing Reagent | 150 mL x 1 bottle |
|---|---|
| Picric Acid Reagent (Picric Acid 22 mmol/L) | 50 mL x 1 bottle |
| 0.75 mol/L Sodium Hydroxide Solution | 50 mL x 1 bottle |
| Standard Solution (Creatinine 10 mg/dL) | 15 mL x 1 bottle |
Overview
※For research purposes only with animal serum/plasma, Not for Diagnostic Use.
Introduction
Creatinine is a metabolite which is produced by creatine phosphate directly or dehydration of creatine in muscle and nerve. It is excreted from the body through kidney glomerular filtration.
Assay principle
LabAssay™ Creatinine can be used to measure the creatinin levels in samples by Jaffe's reaction where creatinine produces quantitatively an orange color with picric acid in alkaline condition.
Performance
| Sample | Serum, plasma (Mouse, Rat) |
|---|---|
| Standard curve range | 2.5~10 mg/dL (mg/100 mL) |
| Measurement time | Approx. 40 min. |
| Amount of sample | 50 μL |
| Measurement wavelength | 520 nm |
Standard Curve
Procedure
Perform the assay in the wells according to the following table scheme.
| Test | Standard | Blank | |
|---|---|---|---|
| First operations should be performed in the sample tubes. | |||
| Sample | Sample 50 μL |
Standard solution 50 μL |
Water 50 μL |
| Deproteinizing reagent | 300 μL | 300 μL | 300 μL |
| Mix well, and stand at room temperature for 10 min. Centrifuge over 2500 rpm for 10 min. Subsequent operations should be performed in the wells. |
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| Supernatant | 100 μL | 100 μL | 100 μL |
| Stand at 25 ℃ - 30 ℃ | |||
| Picric Acid Reagent | 50 μL | 50 μL | 50 μL |
| 0.75 mol/L Sodium Hydroxide Solution | 50 μL | 50 μL | 50 μL |
| Stand at 25 ℃ - 30 ℃ for 20 min. Measure the absorbance of the test sample and standard solution at 520nm with the blank solution as the control within the next 30 min. |
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Data
Spike-and-Recovery Test
Spike and Recovery Test of Creatinine, a known amount of standard is spiked into in mouse and rat sample n=2.
| Rat Sample | Spike (mg/dL) | Mean (mg/dL) | Recovery (mg/dL) | Recovery (%) |
|---|---|---|---|---|
| Serum | - | 2.86 | - | - |
| 3.27 | 5.61 | 2.75 | 84.1 | |
| 5.60 | 8.13 | 5.27 | 94.1 | |
| 7.35 | 9.34 | 6.48 | 88.2 | |
| Plasma (EDTA) |
- | 2.78 | - | - |
| 2.79 | 5.22 | 2.44 | 87.5 | |
| 4.78 | 8.3 | 5.52 | 115 | |
| 6.27 | 9.22 | 6.44 | 103 | |
| Plasma (Heparin) |
- | 2.88 | - | - |
| 3.17 | 5.53 | 2.65 | 83.6 | |
| 5.43 | 8.25 | 5.37 | 98.9 | |
| 7.13 | 9.27 | 6.39 | 89.6 |
| Mouse Sample | Spike (mg/dL) | Mean (mg/dL) | Recovery (mg/dL) | Recovery (%) |
|---|---|---|---|---|
| Serum | - | 2.68 | - | - |
| 3.00 | 5.90 | 3.22 | 107 | |
| 5.14 | 8.08 | 5.40 | 105 | |
| 6.74 | 8.90 | 6.22 | 92.3 | |
| Plasma (EDTA) |
- | 3.14 | - | - |
| 2.71 | 5.63 | 2.49 | 91.9 | |
| 4.65 | 7.74 | 4.60 | 98.9 | |
| 6.10 | 8.25 | 5.11 | 83.8 | |
| Plasma (Heparin) |
- | 3.01 | - | - |
| 2.85 | 5.48 | 2.47 | 86.7 | |
| 4.88 | 7.74 | 4.73 | 96.9 | |
| 6.41 | 8.60 | 5.59 | 87.2 |
Linearity-of-Dilution Test
- Rat sample
- Mouse sample
Example of measurement
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Normal Rat serum, Sprague Dawley, male No. Measured(mg/dL) mean SD CV(%) 1 0.553 0.546 0.550 0.0049 0.90 2 0.716 0.824 0.770 0.076 9.9 3 1.12 1.26 1.19 0.095 8.0 4 1.22 1.29 1.25 0.050 4.0 5 1.04 0.994 1.01 0.029 2.9 -
Normal Rat plasma K2EDTA, Sprague Dawley, male No. Measured(mg/dL) mean SD CV(%) 1 0.319 0.288 0.304 0.022 7.2 2 0.638 0.627 0.633 0.0078 1.2 3 0.824 0.871 0.848 0.033 3.9 4 0.661 0.681 0.671 0.014 2.1 5 0.205 0.233 0.219 0.020 9.0
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Normal Mouse serum, CD-1(ICR), male No. Measured(mg/dL) mean SD CV(%) 1 0.199 0.205 0.202 0.0042 2.1 2 0.492 0.507 0.500 0.011 2.1 3 0.734 0.722 0.728 0.0085 1.2 4 0.641 0.716 0.679 0.053 7.8 5 0.192 0.219 0.206 0.019 9.3 -
Normal Mouse plasma K2EDTA, CD-1(ICR), male No. Measured(mg/dL) mean SD CV(%) 1 0.0950 0.103 0.0990 0.0057 5.7 2 0.607 0.559 0.583 0.034 5.8 3 0.556 0.528 0.542 0.020 3.7 4 0.490 0.546 0.518 0.040 7.6 5 0.113 0.110 0.112 0.0021 1.9
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Normal Canine serum, Beagle, male No. Measured(mg/dL) mean SD CV(%) Age(years) 1 1.67 1.48 1.57 0.13 8.4 4 2 1.16 1.07 1.11 0.063 5.7 6 3 1.54 1.41 1.48 0.092 6.2 6 4 1.58 1.40 1.49 0.13 8.9 11 5 1.46 1.49 1.47 0.023 1.6 12 6 1.17 1.20 1.19 0.027 2.3 6 7 1.16 1.20 1.18 0.027 2.3 10 8 1.23 1.30 1.27 0.047 3.7 4 -
Normal Canine plasma EDTA, Beagle, male No. Measured(mg/dL) mean SD CV(%) Age(years) 1 1.48 1.45 1.46 0.021 1.5 4 2 1.21 1.18 1.20 0.024 2.0 6 3 1.33 1.22 1.27 0.071 5.6 10 4 1.56 1.49 1.53 0.048 3.2 12 5 1.31 1.29 1.30 0.0099 0.76 6 6 1.04 1.04 1.04 0.0028 0.27 11 7 1.14 1.16 1.15 0.013 1.2 10 8 1.26 1.29 1.28 0.019 1.5 12
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Normal Feline serum, Short hair, male No. Measured(mg/dL) mean SD CV(%) Age(years) 1 1.86 1.78 1.82 0.060 3.3 3.0 2 1.58 1.53 1.55 0.034 2.2 3.0 3 1.81 1.68 1.75 0.092 5.3 6.0 4 2.17 1.95 2.06 0.16 7.6 4.0 5 1.88 1.92 1.90 0.030 1.6 6.0 6 1.89 1.86 1.87 0.017 0.91 6.0 7 1.71 1.76 1.73 0.040 2.3 1.0 8 1.48 1.56 1.52 0.055 3.6 1.0 -
Normal Feline plasma EDTA, Short hair, male No. Measured(mg/dL) mean SD CV(%) Age(years) 1 1.63 1.61 1.62 0.011 0.65 0.5 2 1.41 1.36 1.39 0.038 2.8 1.0 3 1.78 1.62 1.70 0.11 6.7 3.0 4 1.97 1.76 1.87 0.15 7.9 0.5 5 1.66 1.67 1.66 0.0085 0.51 3.0 6 1.28 1.29 1.29 0.0028 0.22 3.0 7 1.26 1.30 1.28 0.029 2.3 0.5 8 1.60 1.71 1.65 0.080 4.8 1.0
References
- Kawamoto, T. et al. : Glycative Stress Res., 3, 15 (2016). Relationships between urinary melatonin metabolites and glycative stress and body functional age *Human urine
- Guan, Y. et al. : J. Pharmacol. Sci., 135, 81 (2017). A protease-activated receptor-1 antagonist protects against podocyte injury in a mouse model of nephropathy *Mouse urine and plasma
- Ito, K. et al. : Biol. Pharm. Bull., 38, 1169 (2015). Increased Milk Protein Concentration in a Rehydration Drink Enhances Fluid Retention Caused by Water Reabsorption in Rats *Rat urine and plasma
- Tahara, Y. et al. : Med. Chem. commun., 8, 415 (2017). Encapsulation of a nitric oxide donor into a liposome to boost the enhanced permeation and retention (EPR) effect *Mouse Serum
- Nasrin, S. et al. : J. Pharmacol. Sci., 122, 270 (2013). Beneficial Effects of Gosha-jinki-gan and Green Tea Extract in Rats With Chemical Cystitis *Rat urine
- Toyama, K. et al. : Br. J. Pharmacol., 166, 1183 (2012). Loss of multidrug and toxin extrusion 1 (MATE1) is associated with metformin-induced lactic acidosis *Mouse plasma
Overview / Applications
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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