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Iba1 (Ionized calcium-binding adapter molecule1) is an approximately 17 kDa calcium-binding protein. It is used as a microglial marker because it is expressed specifically in microglia in the central nervous system¹⁾. It is expressed in both resting and activated microglia but is reportedly expressed more highly in activated microglia²⁾. It is also expressed in macrophages in peripheral tissues and is known as AIF-1 (Allograft inflammatory factor-1). Iba1 binds to F-actin in cells to form actin bundles. The formation of actin bundles is thought to be required for the membrane ruffling observed during cell migration and phagocytosis³⁾.
As well as the existing Anti Iba1, Rabbit (for Immunocytochemistry) (Product No. 019-19741), we offer the new one labeled with SCPIA Dye™ 594, a proprietary fluorescent dye. It enables you to save time to detect with a secondary antibody.

　

Don't miss this opportunity!

　

　

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Product Overview

• Antibody Information

  Clonality	Polyclonal
  Antigen	Synthetic peptide (Iba1 C-terminal sequence)
  Host	Rabbit
  Reactivity	Mouse, Rat
  Buffer	PBS, 0.05% sodium azide
  Conjugate	SPICA Dye™ 594　(Ex=575 nm, Em=611 nm)
  Applications	Immunohistochemistry (Frozen Section) 1:200 - 2000

• Application Data: Immunohistochemistry

  • Rat
    

    

  • Mouse
    

    

  Species: Rat / Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:200

Selection guide

References Imai, Y., Ibata, I., Ito, D., Ohsawa, K., & Kohsaka, S.: Biochem.Biophys. Res. Commun., 224(3), 855(1996). A Novel Geneiba1 in the Major Histocompatibility Complex Class III Region Encoding an EF Hand Protein Expressed in a Monocytic Lineage Mori, I., Imai, Y., Kohsaka, S., & Kimura, Y.: Microbiol. Immunol., 44(8), 729(2000). Upregulated expression of Iba1 molecules in the central nervous system of mice in response to neurovirulent influenza A virus infection Sasaki, Y., Ohsawa, K., Kanazawa, H., Kohsaka, S., & Imai, Y. Biochem. Biophys. Res. Commun., 286(2), 292(2001). Iba1 is an actin-cross-linking protein in macrophages/microglia.