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Great value starter sets of MagCapture™ Exosome Isolation Kit PS Ver. 2

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FUJIFILM Wako is proud to provide the technology to isolate highly purified exosomes.
This Exosome Isolation Starter Set is available for a limited time.
Don't miss this opportunity!

Promotion Overview

Exosome Starter Set A Exosome Starter Set B
Product Name 290-84103: MagCapture™ Exosome Isolation Kit PS Ver.2
058-09261: EV-Save™ Extracellular Vesicle Blocking Reagent
290-84103: MagCapture™ Exosome Isolation Kit PS Ver.2
053-09451: EV-Up™ EV Production Basal Medium for MSC, AF
298-84001: EV-Up™ MSC EV Production Supplement, AF
Promotion Price USD 703.00 ▶ USD 595.00 15%OFF USD 925.00 ▶ USD 595.00 35%OFF
Promotion Period 1st July, 2021 ~ 31st August, 2021
Notice
  • This promotion will apply to orders placed through our online store.
  • When checking out, please specify in the shipping note which exosome starter set you want to purchase.
  • These starter sets contain certain components need to be stored on dry ice. Products will be shipped in a dual temperature box, with the most temperature-sensitive products placed in a separate dry ice compartment.

Starter Set Details

Exosome Starter Set A for general purposes - 15% OFF

Product Code Product Name Package Size Regular Price Storage
1 290-84103 MagCapture™ Exosome Isolation Kit PS Ver.2 10 tests USD 595.00 2 ~ 10 degrees C
2 058-09261 EV-Save™ Extracellular Vesicle Blocking Reagent 1 mL USD 108.00 - 20 degrees C
Features of EV-Save™ Extracellular Vesicle Blocking Reagent
  • Strong suppression of extracellular vesicles adsorption to laboratory tools
  • Simple operation: just to add to the sample

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Exosome Starter Set B for MCS cell users - 35% OFF

Product Code Product Name Package Size Regular Price Storage
1 290-84103 MagCapture™ Exosome Isolation Kit PS Ver.2 10 tests USD 595.00 2 ~ 10 degrees C
2 053-09451 EV-Up™ EV Production Basal Medium for MSC, AF 95 mL USD 132.00 2 ~ 10 degrees C
3 298-84001 EV-Up™ MSC EV Production Supplement, AF for 100 mL USD 198.00 - 20 degrees C
Features of EV-Up™ EV Production Basal Medium for MSC, AF & MSC EV Production Supplement, AF
  • Provides higher EV yield compared to conventional serum-containing media
  • Enables production of EVs with high activity
  • Maintains MSC viability in culture

More details

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Limited Time Promotion

  • This promotion will apply to orders placed through our online store.
  • This promotion is open to customers in the US and Canada.
  • When checking out, please specify in the shipping note which exosome starter set want to purchase.
  • Limited to 5 sets per customer.
  • Discount will be applied to orders received by FUJIFILM Wako Chemicals until 31st August 2021, or until promotional supplies sell out.
  • Discount applies to the list price in effect at the time of receipt of the order and cannot be combined with other discounts or promotions.
  • These starter sets contain certain components that need to be stored on dry ice. Products will be shipped in a dual temperature box, with the most temperature-sensitive products placed in a separate dry ice compartment.

Different points between V2.0 kit*1 (Ver.2) and conventional kit*2

*1: V2.0 kit = MagCapture™ Isolation Kit PS Ver.2 (Code No. 290-84103)
*2: Conventional kit = MagCapture™ Isolation Kit PS (Code No. 293-77601)

  • Improved exosome recovery efficiency
  • Shortened experiment time
    One hour reaction versus three hours with the conventional kit
  • Reduced cell cytotoxicity
    Does not require buffer exchange

Outline of Procedure of V2.0 kit

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Kit components of V2.0 kit

2 tests 10 tests
Biotin Capture Magnetic Beads 120 μL 600 μL
Biotin-labeled Exosome Capture 20 μL 100 μL
Exosome Immobilizing / Washing Buffer (10 x) 5 mL 25 mL
Exosome Binding Enhancer (500 x) 300 μL 1500 μL
Exosome Elution Buffer (10 x) 300 μL 1500 μL
Reaction Tubes 4 tubes 22 tubes

Comparison with the conventional kit

Exosome recovery efficiency: serum and plasma

Exosome recovery efficiency: culture supernatant of mesenchymal stem cells (MSC)

Cell cytotoxicity of purified exosome

Exosome recovery efficiency: serum and plasma

The following assays were performed on EVs isolated from human and serum plasma, using either the conventional kit or the V2.0 kit.

  1. Comparison of EV marker expression using the PS Capture™ Exosome ELISA Kit
  2. Comparison of EV marker by western blot
  3. Measuring particle numbers by Nanosight

V2.0 kit contains an exosome elution buffer (10 x). In these assays, 1 x elution buffer and 2 x elution buffer were evaluated.
As a result, V2.0 kit is as good as the conventional kit human blood samples. In particular, recovery efficiency of EV from plasma samples improved with 2 x elution buffer.

1. Comparison of EV marker expression (PS Capture™ Exosome ELISA kit)

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2. Comparison of EV markers (western blot)

▷Detection antibody: Anti CD9, Rat Monoclonal Antibody (77B) (Code No. 019-28173)

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▷Detection antibody:Anti CD63, Monoclonal Antibody (3-13) (Code No. 012-27063)

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▷Detection Antibody: Anti Flotillin2, Monoclonal Antibody (BD)

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3. Measuring particle numbers by Nanosight
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Exosome recovery efficiency: culture supernatant of mesenchymal stem cells (MSC)

The following assays were performed on EVs isolated from the culture supernatant of MSCs, using either the conventional kit or the V2.0 kit.

  1. Comparison of EV marker expression using the PS Capture™ Exosome ELISA Kit
  2. Comparison of EV marker by western blot
  3. Measuring particle number by Nanosight

As a result, EV recovery efficiency of V2.0 kit is higher than that of the conventional kit.

1. Comparison of EV marker (PS Capture™ Exosome ELISA Kit)

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2. Comparison of EV markers expression (western blot)
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3. Measuring particle numbers by Nanosight

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Cell cytotoxicity of purified exosomes

EVs from COLO201 culture supernatant were isolated and purified by conventional kit or the V2.0 kit. Equal amounts of purified EVs and the exosome elution buffer were added to human normal fibroblasts. After 48 hours, morphological cell changes and cell death were observed with the conventional kit. However, these changes were not observed with the V2.0 kit. Therefore, the V2.0 kit could be used for uptake experiments (in vitro or in vivo), without buffer exchange.*3.

*3: Buffer exchange is recommended if EDTA in the elution buffer poses a problem.

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