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Which BDNF are you interested in?

BDNF (Brain-Derived Neurotrophic Factor) is expressed as precursor BDNF (proBDNF), transferred to the secreting site, and processed to mature BDNF (mBDNF). ProBDNF has been discovered to have a different physiologic role from mBDNF. mBDNF induces long-term potentiation (LTP) via TrkB and enhances neurogenesis and development whereas proBDNF induces long-term depression (LTD) via low-affinity receptor p75^NTR and promotes apoptosis. Therefore, BDNF studies require detection methods that are specific to each molecule.

　

Why is it difficult to specifically detect mBDNF?

Since mBDNF is truncated from proBDNF, it does not have unique amino acid sequences. To address this issue, we have developed Mature BDNF ELISA Kit Wako, using an antibody that specifically detects the N-terminus of mBDNF. This antibody has as low as about 10% cross-reactivity to proBDNF, enabling the detection of mBDNF with higher specificity and sensitivity than other commercially available kits.

　

Comparison with conventional kit

　

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Specification Overview of Mature BDNF ELISA kit Wako

• Standard curve range	4.1 - 1,000 pg/mL
  Specificity	mBDNF*
  Sample	Human serum and plasma**
  Sample volume	Human serum：10 μL Human plasma：5 μL
  Assay time	4 hours
  Detection method	Colorimetric method

  * Approximately 10% reaction to proBDNF
  ** This antibody can also detect mouse and rat BDNF.

• 

What is BDNF? BDNF, a member of the neurotrophin family, is widely expressed in the brain from the developing stage to the maturing stage of the neurological system. Enhancing neuron survival and synapse formation are well-known functions of BDNF. As such, BDNF has been getting attention in the neuroscience community, as it is an important factor for the development and maintenance of neurological function. BDNF has been also reported to be deeply involved in brain-derived disorders, including psychiatric disorders, and is expected to be a biomarker particularly in depression research. Studies have reported that serum BDNF levels in depression patients are lower than in healthy individuals. Moreover, as depression patients not taking antidepressants show a reduction in serum BDNF, quantitation of BDNF is a possible indicator for depression treatment. Recently, meta-analyses have also unveiled a reduction in serum BDNF levels in patients with bipolar disorder and schizophrenia, as well as depression.

　

Measurement of Serum mBDNF of Patients with Psychiatric Diseases

• Healthy Control (n=6)

  No.	Sex	Age	Assay value (ng/mL)
  1	F	48	14.5
  2	F	50	22.8
  3	F	54	20
  4	F	47	18.5
  5	F	42	13.8
  6	F	60	19.1

• Major depression (n=6)

  No.	Sex	Age	Assay value (ng/mL)
  1	F	66	15.7
  2	F	38	18.1
  3	F	43	10
  4	F	52	16.6
  5	F	41	22.3
  6	F	58	15.1

• Bipolar disorder (n=4)

  No.	Sex	Age	Assay value (ng/mL)
  1	F	46	16.5
  2	F	52	20.9
  3	F	52	11.5
  4	F	52	11.5

• Schizophrenia (n=4)

  No.	Sex	Age	Assay value (ng/mL)
  1	F	36	17.9
  2	F	53	23.0
  3	F	55	14.6
  4	F	51	10.8

　

Healthy Control	Major depression	Bipolar disorder	Schizophrenia
18.1±3.41	16.3±4.02	15.1±4.53	16.6±5.17

(ng/mL)

We have examined mBDNF in serum collected from healthy individuals, and patients with major depression, bipolar disorder, or schizophrenia. These results showed a trend that these patients had lower mBDNF levels than healthy individuals (It is not statistically significant).

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Quality Control

■Dilution linearity test

■Spike and recovery test in human serum and plasma

Sample	Spike mBDNF (pg/mL)	Assay value (pg/mL)	Recovery volume (pg/mL)	Receovery (%)	Sample	Spike mBDNF (pg/mL)	Assay value (pg/mL)	Recovery volume (pg/mL)	Receovery (%)
Human Serum 1	0	330	‐	‐	Human Plasma 1	0	156	‐	‐
	100	423	93.0	93.0		100	252	96.0	96.0
	250	581	251	100		250	407	251	100
	500	817	487	97.4		500	661	505	101
Human Serum 2	0	56.6	‐	‐	Human Plasma 2	0	66.0	‐	‐
	50	106	49.4	98.8		50	115	49.0	98.0
	100	154	97.4	97.4		100	164	98.0	98.0
	200	257	200	100		200	276	210	105
	300	363	306	102		300	369	303	101

　

Kit Component

Antibody-coated Plate　(96well)	1 Plate
Mature BDNF Standard	100 μL
Buffer	60 mL
Biotin-conjugated Antibody Solution	100 μL
Peroxidase-conjugated Streptavidin Solution	100 μL
TMB Solution	6 mL
Stop Solution	6 mL
Wash Solution (10×)	100 mL
Plate Seal	4 Sheets

　

How to Redeem

1. Log in to the site with your username and password.
2. Select item(Catalog# 296-83201) to purchase, and add it to the cart.
3. See the cart and click the "Checkout" button.
4. Enter the promo code, mBDNF2020, in the "Shipping notes" field.
   
5. Click "Next".
6. Complete your purchase.
7. You will receive an invoice with 30 % off after shipment.

Note

• When you purchase it on EC, it will be displayed as USD759, but the actual invoice/packing list will show the discounted price.
• If you do not enter the promo code, you will not be able to receive the discount.
• This promotion is open to customers in the US and Canada. Discount will apply to orders received by FUJIFILM Wako Chemicals USA no later than November 30, 2020, or until promotional supplies are depleted, whichever comes first.