Digitonin is a spirostan saponin obtained from the seeds and leaves of digitalis (Digitalis purpurea, Digitalis lanata). A mild nonionic detergent, digitonin is used in a wide range of applications, including solubilization of membrane proteins, permeabilization of cellular membranes, isolation of mitochondria and quantitation of cholesterol.
Digitonin enables the isolation of neurotransmitter receptors, such as dopamine receptors, beta adrenergic receptors, and GABA receptors. Digitonin has also been used in cellular membrane analysis by Blue-Native PAGE and Cryo-EM.
Although Digitonin is sometimes considered difficult to use due to its low solubility in water, we provide water-soluble Digitonin for Biochemistry, prepared by an original, proprietary purification method.