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SI traceable certified reference material

Amino Acid Standard (CRM)

Amino acids are components that make up proteins, and are important nutritional components contained in various foods such as meat and grains. In recent years, many drinks and supplements containing amino acids become popular, and the function of amino acids contained in foods has been gathering attention.
By measuring amino acids in blood samples, the development of technology for screening the possibility of future illness such as cancer, diabetes, and stroke is in progress. There is a demand for more accurate quantification of amino acid contents in samples.

In 2019, FUJIFILM Wako acquired the "Amino acid mixture standard material producer" based on ASNITE certification for the first time in Japan, and have started the production of amino acid CRM that can be "measurement" for amino acid measurement.
We newly released CRM amino acid mixture standard solutions. These products are SI traceable, and the reliability of the measured values ​​is guaranteed internationally.

Features

  • The first amino acid mixture standard solutions based on ISO 17034 (according to the accreditation program by ASNITE) in Japan
  • Using NMIJ CRM and our SI traceable standard TRM (Traceable Reference Material) as raw material
  • Minimal difference between lots
  • Attached the certificate that describes impurities of amino acids
  • Guaranteed the uncertainty obtained from stability and homogeneity

Certificate

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Composition

Type P-Ser Tau PEA Urea Asp Hyp Thr Ser Asn Glu Gln Sar Aad Pro Gly Ala Clt Abu Val (Cys)2 Met Hcy
(Ala)
Ile Leu Tyr
AN
B
H
APDS1
APDS2
Type Phe bAla bAlb 4Abu Trp MEA NH4 5Hyl Orn Lys 1Mehis His 3Mehis Ans Car Arg
AN
B
H
APDS1
APDS2

AN…Amino Acids Mixture Standard Solution, Type AN [CRM] (Code No. 015-27891)
B …Amino Acids Mixture Standard Solution, Type B [CRM] (Code No. 011-27871)
H …Amino Acids Mixture Standard Solution, Type H [CRM] (Code No. 018-27881)
APDS1…APDSTAG® Wako Amino Acids Mixture Standard Solution No.1 [CRM] (Code No. 017-27851)
APDS2…APDSTAG® Wako Amino Acids Mixture Standard Solution No.2 [CRM] (Code No. 014-27861)

Abbreviations

Abbreviation Amino acids Abbreviation Amino acids Abbreviation Amino acids
P-Ser O-Phosphoserine Ala Alanine MEA 2-Aminoethanol
Tau Taurine Cit Citrulline NH4 Ammonium Ion
PEA O-Phosphoethanolamine Abu 2-Aminobutyric Acid 5Hyl 5-Hydroxylysine
Urea Urea Val Valine Orn Ornithine
Asp Aspartic Acid (Cys)2 Cystine Lys Lysine
Hyp Hydroxyproline Met Methionine 1Mehis 1-Methylhistidine
Thr Threonine Hcy(Ala) Cystathionine His Histidine
Ser Serine Ile Isoleucine 3Mehis 3-Methylhistidine
Asn Asparagine Leu Leucine Ans Anserine
Glu Glutamic Acid Tyr Tyrosine Car Carnosine
Gln Glutamine Phe Phenylalanine Arg Arginine
Sar Sarcosine bAla β-Alanine
Aad 2-Aminoadipic Acid bAib 3-Aminoisobutyric Acid
Pro Proline 4Abu 4-Aminobutyric Acid
Gly Glycine Trp Tryptophan

Measurement Method

APDS Method

The APDS (3-aminopyridyl-N-hydroxysuccinimidyl carbamate) method is a new high-speed amino acid analysis method that can measure amino acid analysis, which used to take two hours, in a few minutes. In the APDS method, it is derivatized by APDS, separated by HPLC, and detected by MS. Even if the retention time in the chromatogram is the same, each amino acid can be distinguished by m/z, so the analysis time can be significantly reduced.

Ninhydrin Method

The ninhydrin method is a post-column method in which each amino acid is separated by HPLC, derivatized with ninhydrin, and the absorbance is measured. It is an amino acid analysis method that is often used and has excellent quantitative and reproducibility.

PTC Method

The PTC (phenylthiocarbamoyl) method is a derivatization method using PITC (phenylisothiocyanate) reagent (Code No. 162-08473) and reacts with primary and secondary amines. The PTC method has the advantage that the molar extinction coefficient of the derivatized compound is almost the same as all amino acids. Gradient elution is required for simultaneous analysis of multi-component amino acids, but by using Wakopak® Wakosil® -PTC and a special eluent, it is possible to easily separate and analyze amino acids with good reproducibility.

Product List

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Amino Acids Mixture Standard Solution, Type AN [CRM]
O-Phosphoserine 0.1125~0.1375 μmol/mL Sarcosine 0.5625~0.6875 μmol/mL L-Methionine 0.2250~0.2750 μmol/mL
Taurine 0.1125~0.1375 μmol/mL L-Proline 0.2250~0.2750 μmol/mL L-Cystathionine 0.1125~0.1375 μmol/mL
O-Phosphoethanolamine 0.1125~0.1375 μmol/mL Glycine 0.2250~0.2750 μmol/mL L-Isoleucine 0.2250~0.2750 μmol/mL
Urea 4.500~5.500 μmol/mL L-Alanine 0.2250~0.2750 μmol/mL L-Leucine 0.2250~0.2750 μmol/mL
L-Aspartic Acid 0.2250~0.2750 μmol/mL L-Citrulline 0.2250~0.2750 μmol/mL L-Tyrosine 0.2250~0.2750 μmol/mL
L-Hydroxyproline 0.2250~0.2750 μmol/mL DL-2-Aminobutyric Acid 0.1125~0.1375 μmol/mL L-Phenylalanine 0.2250~0.2750 μmol/mL
L-Threonine 0.2250~0.2750 μmol/mL L-Valine 0.2250~0.2750 μmol/mL β-Alanine 0.2250~0.2750 μmol/mL
L-Serine 0.2250~0.2750 μmol/mL L-Cystine 0.1125~0.1375 μmol/mL DL-3-Aminoisobutyric Acid 0.2250~0.2750 μmol/mL
L-Glutamic Acid 0.2250~0.2750 μmol/mL
Amino Acids Mixture Standard Solution, Type B [CRM]
4-Aminobutyric Acid 0.2250~0.2750 μmol/mL 1-Methyl-L-histidine 0.2250~0.2750 μmol/mL
2-Aminoethanol 0.2250~0.2750 μmol/mL L-Histidine 0.2250~0.2750 μmol/mL
Ammonium Chloride 0.2250~0.2750 μmol/mL 3-Methyl-L-histidine 0.2250~0.2750 μmol/mL
5-Hydroxy-DL-lysine 0.2250~0.2750 μmol/mL L-Anserine 0.2250~0.2750 μmol/mL
L-Ornithine 0.2250~0.2750 μmol/mL L-Carnosine 0.2250~0.2750 μmol/mL
L-Lysine 0.2250~0.2750 μmol/mL L-Arginine 0.2250~0.2750 μmol/mL
Amino Acids Mixture Standard Solution, Type H [CRM]
L-Aspartic Acid 0.2250~0.2750 μmol/mL L-Methionine 0.2250~0.2750 μmol/mL
L-Threonine 0.2250~0.2750 μmol/mL L-Isoleucine 0.2250~0.2750 μmol/mL
L-Serine 0.2250~0.2750 μmol/mL L-Leucine 0.2250~0.2750 μmol/mL
L-Glutamic Acid 0.2250~0.2750 μmol/mL L-Tyrosine 0.2250~0.2750 μmol/mL
L-Proline 0.2250~0.2750 μmol/mL L-Phenylalanine 0.2250~0.2750 μmol/mL
Glycine 0.2250~0.2750 μmol/mL L-Lysine 0.2250~0.2750 μmol/mL
L-Alanine 0.2250~0.2750 μmol/mL L-Histidine 0.2250~0.2750 μmol/mL
L-Cystine 0.1125~0.1375 μmol/mL Ammonium Chloride 0.2250~0.2750 μmol/mL
L-Valine 0.2250~0.2750 μmol/mL L-Arginine 0.2250~0.2750 μmol/mL

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APDS Method

Ninhydrin Method (for Hitachi High-speed Amino Acid Analysis)

Ninhydrin Method (for JEOL)

PTC Method

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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