GenomONE®-Neo EX are novel and unique transfection kits which employ the membrane fusion ability of the envelope of Sendai virus (Hemagglutinating virus of Japan: HVJ). HVJ Envelope (HVJ-E) is a nonproliferative and noninfectious vesicle purified after complete inactivation of Sendai virus genomic RNA. HVJ-E can introduce a variety of molecules (plasmid DNA, siRNA/miRNA, protein) into cells via membrane fusion.
This kit is manufactured by ISHIHARA SANGYO KAISHA (ISK) in Japan. If you are interested in this kit and getting further product information, please check ISK website.
What is GenomONE® Series (HVJ-E)?
HVJ Envelope(HVJ-E) is a novel and unique vesicle that employs the membrane fusion ability of the envelope of Sendai virus (Hemagglutinating virus of Japan; HVJ). The genome RNA of HVJ is completely inactivated to use HVJ-E in biosafety level 1(BSL1) laboratories.
HVJ-E is known for its ability to incorporate a variety of molecules, such as plasmid DNA, siRNA/miRNA or proteins and introduce them into the cytoplasm via membrane fusion. HVJ-E can also be used as a cell fusion reagent.
- HVJ-E can incorporate various molecules (plasmid DNA, siRNA/miRNA, proteins).
- HVJ-E-incorporated molecules are introduced into cells via membrane fusion.
- HVJ-E is also applicable to in vivo transfection.
Application data; Protein and in vivo Transfection
Intrathecal administration of siRNA to rats
By using HVJ-E vector, NK1R siRNA was administered into the spinal cord. Immunohistochemical reaction of NK1R was suppressed by siRNA-treated during 1 to 7 days.
Data provided by
- Dr. Rumi Naono-Nakayama
Division of Anatomy and Cell Biology, Faculty of Medicine, Tohoku Medical and Pharmaceutical University (Japan)
- Dr. Toshikazu Nishimori
Department of Psychiatry, Faculty of Medicine, University of Miyazaki (Japan)
Eur. J. Pharmacol., 670, 448-457 (2011).
Intracellular delivery of Cre recombinase protein
By introducing Cre recombinase into 2-2 cells with GenomONE®-Neo EX, loxp sites inserted in the genome sequence were deleted and Lac Z gene expression was induced.
2-2 (monkey, African green, RIKEN BioResource Center): 35 copies of PCAG-loxP-neopA-loxP-LacZ were tandemly inserted into the genome.
- Open All
- Close All
Product content may differ from the actual image due to minor specification changes etc.