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KANEKA KanCap™

What is KanCap™?

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highly cross-linked cellulose beads

KANEKA KanCap™ is an affinity chromatography resin designed for antibody purification. It is based on the unique combination of novel recombinant ligands and innovative highly cross-linked cellulose beads.

It can be used to purify a wide variety of antibody fragments, including immunoglobulin, single-chain variable fragment (scFv), F(ab'), and F(ab')2, from biological samples and cell culture supernatants.

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KANEKA KanCapA™

KANEKA KanCapA™ is a Protein A affinity chromatography resin designed for the purification of immunoglobulin and recombinant antibodies including Fc region from biological samples and cell culture supernatants.

KANEKA KanCapA™ is composed of highly cross-linked cellulose beads and a novel alkaline-resistant Protein A ligand, and can be used for affinity purification of IgG.

Modified Protein A
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Features

  • Alkaline stability
  • Elution can be performed at mild pH (e.g. pH 3.5)
  • High dynamic binding capacity: ≧35 mg IgG/mL
  • Easy to scale-up
  • Can be re-used

Protein A used in the product:
  1. C-domain based pentamer
  2. Enhanced alkaline stability
  3. Reduced non-specific binding to Fab
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Sharp elution of antibody under optimal pH

Binding affinity to various animal-derived antibodies

Species, Class Binding Species, Class Binding Species, Class Binding
Human IgG1 +++ Mouse IgG3 +++ Rat IgG3
Human IgG2 +++ Mouse IgA Rat IgM
Human IgG4 +++ Rat IgG1 + Porcine IgG (poly) ++
Human IgM Rat IgG2a Canine IgG (poly) +++
Mouse IgG1 + Rat IgG2b Sheep IgG (poly) +
Mouse IgG2a +++ Rat IgG2c ++ Goat IgG (poly) +
Mouse IgG2b ++

+++: Strong Binding ++: Medium Binding +: Weak Binding -: No binding

KANEKA KanCapA™ 3G

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KANEKA KanCapA™ 3G is composed of novel highly cross-linked cellulose beads and an alkaline-resistant Protein A resin, and has a high dynamic binding capacity and enhanced ability to remove impurities.

It can be used to purify monoclonal antibodies and Fc-fusion chimeric proteins from biological samples and cell culture supernatants under mild conditions.

Features

  • Alkaline stability
  • Wider range of elution pH
  • High dynamic binding capacity: ≧58 mg IgG/mL
  • Excellent ability to remove impurities
  • Easy to scale-up
  • Can be re-used

KANEKA KanCap™ G

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KANEKA KanCap™ G is a pre-packed column filled with resin for affinity chromatography. It is designed to capture and purify antibody fragments containing CH1 domain, including F(ab’) and F(ab’)2.

It is particularly useful for the purification of low-molecular-weight antibodies (e.g. Fab) that lack Fc region and are therefore difficult to purify with Protein A.

Features

  • High affinity to antibodies that contain CH1 and Fc regions
  • High antibody recovery rate

Design of KANEKA KanCap™ G

KANEKA KanCap™ G uses a novel Protein G, which has an enhanced affinity to antibody fragment containing CH1 domain (Fab) compared with its naïve form. It exhibits high affinity to Fab that consists of either κ or λ light chain.

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KANEKA KanCap™ L

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KANEKA KanCap™ L is a pre-packed column filled with resin for affinity chromatography. It is designed to capture and purify various forms of antibody fragments, including single-chain variable fragment (scFv), F(ab’), and F(ab’)2.

It has an enhanced affinity to VL domain of κ light chain and is also effective for the purification of full-length antibody that binds poorly to Protein A.

Features

  • High affinity to antibodies that include κ light chain
  • Binds to wide range of antibodies that include κ light chain

Design of KANEKA KanCap™ L

After creating and screening for Protein L mutants, the mutant with the widest binding spectrum was selected to design KANEKA KanCap™ L. It may facilitate the recovery of antibodies with κ light chain that do not bind to wild type Protein L.

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Product List

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Protein A carrier

Protein G carrier

Protein L carrier

Related Product List

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Product content may differ from the actual image due to minor specification changes etc.

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