Lysyl Endopeptidase®, Biochemistry Grade (Lys-C)

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Lysyl endopeptidase® is a serine protease that cleaves peptide bonds at the carboxy-terminal side of lysine residues with a high degree of specificity. It is inhibited by TLCK, PMSF, and DFP but not by TACK. Taking advantage of its excellent specificity, lysyl endopeptidase® is used for peptide fragmentation and peptide mapping for analysis of the primary structure of proteins.

Product Outline

  • Molecular weight: 27,000(Gel filtration), 30,000(SDS-PAGE)
  • Optimal pH: 9.0~9.5(Amidase activity)
  • Isoelectric point: 6.9~7.0
  • Substrate specificity
    ・Hydrolysable substrates・・・Tos-Lys-OMe, Bz-Lys-NH2, Bz-Lys-pNA, Lys-pNA
    ・Unhydrolysable substrates・・・Bz-Arg-NH2, Bz-Arg-pNA, Arg-pNA
  • Inhibitors: DFP, PMSF, TLCK

References

  1. Masaki, T., Nakamura, K., Isono, M. and Soejima, M. : Agric. Biol. Chem., 42, 1443(1978).
  2. Morihara, K., Oka, T., Tsuzuki, H., Tochino, Y. and Kanaya, T. : Biochem. Biophys. Res. Commun., 92, 396(1980).
  3. Schwert, G. W. and Takenaka, Y. : Biochim. Biophys. Acta, 16, 570(1955).
  4. Tuppy, H., Wiesbauer, U. and Wintersberger, E. : HoppeSeyler’s Z. Physiol. Chem., 329, 278(1962).
  5. Masaki, T., Tanabe, M., Nakamura, K. and Soejima, M. : Biochim. Biophys. Acta, 660, 44(1981).
  6. Masaki, T., Fujihashi, T., Nakamura, K. and Soejima, M. : Biochim. Biophys. Acta, 660, 51(1981).

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Recombinant, Biopharmaceutical Analysis Grade (rLys-C)

Mass Spectrometry Grade

Product content may differ from the actual image due to minor specification changes etc.

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