Leptin is a 16 kDa protein mostly produced in white fat tissue, and also expressed in brown fat tissue, placenta, ovary, skeletal muscle, fundus of the stomach, epithelial cells of mammary gland, bone marrow,pituitary gland, and liver. Leptin was first found in mutant obese mouse and proved to be an important hormone relating to energy balance through controlling apetite and metabolism. In amino acid sequence of leptin, 97 % homology is observed between mouse and rat. Blood leptin level is decreased in fasting or by low calorie meal. Postprandial increase of blood leptin level is not evident, however, it rises in obesity or proliferation of adipose tissue reflecting the volume of body fat. Leptin enters the brain and suppresses NPY/AgRP-expressing neuron and activates α-MSH-expressing neuron, causing loss of appetite and food intake. However, highly obese patients, though their blood levels of leptin are very high, show leptin-resistance and the anorexic effect of leptin does not work. Lowered production and deficiency of leptin cause insulin resistance, diabetes mellitus, fatty liver, and hypertriglyceridemia. In addition, leptin is considered to be involved in immune reaction to atherosclerosis, sexual cycle-related energy balance, and surfactant production in alveoli of the lung.

LBIS Mouse Leptin ELISA Kit is a sandwich ELISA system for quantitative measurement of mouse leptin.This is intended for research use only.


  • Measurement range 20.6~5000 pg/mL
  • Measurable in a short period (3 hours)
  • A minute quantity of sample is measurable (10 μL with a standard method of operating)
  • All reagents are solution types and are ready to use
  • High measurement accuracy and reproducibility

Kit Components

1) Antibody-coated plate (8 x 12) wells/plate 1 plate
2) Standard mouse leptin solution (5,000 pg/mL) 500 μL
3) Buffer solution 60 mL
4) Biotinylated anti leptin Antibody 200 μL
5) HRP-conjugated streptavidin 200 μL
6) TMB Solution 12 mL
7) Stop Solution 12 mL
8) Wash Solution (x 10) 100 mL
9) Plate Seal 3 seals


Animal Species Substances Cross-reactivity (%)
Mouse Leptin 100
Rat Leptin 31.5
Human Leptin +

+:Cross reaction
ー:No cross reaction
※Cross-reactivity data: at 3000 pg/mL

Assay Procedures


* Guideline of washing volume: 300 μL/well
**Guideline of shaking: 600-1200 rpm for 10 seconds × 3 times


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