CLEM-Red, CLEM-Green Expression Vectors
We are handling fluorescent protein expression vectors applicable to correlative light and electron microscopy (CLEM). The fluorescent proteins (CLEM-Red, CLEM-Green) incorporated in the vectors can recover the fluorescence of electron microscopy samples weakened by osmium oxide with the fluorescence recovery reagents for CLEM (TUK Solution for multicolor). This makes it possible to perform both fluorescence microscopy and electron microscopy on the same section.
Features
| Vector | pCAG (animal cell expression vector) |
|---|---|
| MCS promoter | CAG promoter |
| polyA signal | SV40 polyA |
| antibiotic-resistant gene | Neomycin / G-418 |
| Vector size | Approx. 5.8-7.0 kbp |
Product specification
| Vector | Excitation Max | Emission Max | Product Code | Product Name | Intracellular Localization | Vector Map | Sequence |
|---|---|---|---|---|---|---|---|
| CLEM-Green | 498 nm | 507 nm | 163-28881 | pCLEM-Green-N | - | ||
| 160-28891 | pCLEM-Green-C | - | |||||
| 163-28901 | pCLEM-Green-nuc | Nucleus | |||||
| CLEM-Red | 588 nm | 633 nm | 167-28661 | pCLEM-Red-N | - | ||
| 160-28651 | pCLEM-Red-C | - | |||||
| 165-28461 | pCLEM-Red-mito | Mitochondria | |||||
| 169-28621 | pCLEM-Red-gol | Golgi body | |||||
| 166-28631 | pCLEM-Red-ER | Endoplasmic reticulum | |||||
| 163-28641 | pCLEM-Red-lyso | Lysosome |
Notice to Purchase
FUJIFILM Wako's products are intended for research use only. If you have requirements for sales and /or development of new vectors which were constructed by DNA fragments and/or sequence information of this product, please contact us.
(ffwk-labchem-tec@fujifilm.com)
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CLEM-Green
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.