Nano-Trap

GFP-Trap^®_A・・・・・・・・・・・・　担体：アガロースビーズ
GFP-Trap^®_MA・・・・・・・・・・・　担体：磁気アガロースビーズ
GFP-Trap^®_M・・・・・・・・・・・・　担体：磁気ビーズ
GFP-Trap^® , free protein・・・・・・　 担体なし

「GFP-Trap^®_A」に必要なbuffer類をセット化したGFP融合タンパク質の単離・精製キットです。

• GFT-Trap^®_A：20 reactions (500μL slurry)
• Lysis buffer (Co-lP)：30mL
• RIPA buffer：30mL
• 5×Wash / Dilution buffer：20mL
• Elution buffer：3mL

	GFP-Trap®_A 担体：アガロースビーズ	GFP-Trap®_MA 担体：磁気アガロースビーズ	GFP-Trap®_M 担体：磁気ビーズ
matrix	agarose	agarose	silica (non porous)
色	white	black	brown
粒子サイズ	90μm （クロスリンク4% アガロースビーズ）	40μm	0.5-1μm
結合能 (Binding Capacity)	3-4μg/10μL slurry	3-4μg/10μL slurry	0.25-0.5μg/10μL slurry
磁性	無し	有り	有り
遠心分離　上限	3,000xg	3,000xg	3,000xg
保存液	20% エタノール	20% エタノール	1x PBS, 0.05% sodium azide

• GFP-Trap
• RFP-Trap
• GST-Trap
• Dnmt1-Trap
• MK2-Trap
• p53-Trap
• Mdm4/HdmX-Trap
• Myc-Trap

（残りはメーカーウェブサイトで確認出来ます）

■使用例：免疫沈降 (Immunoprecipitation : IP)

HIGD1A Regulates Oxygen Consumption, ROS Production, and AMPK Activity during Glucose Deprivation to Modulate Cell Survival and Tumor Growth
K.Ameri et al.: Cell Rep. (2015). pii: S2211-1247(15)00033-9.
doi: 10.1016/j.celrep.2015.01.020. PMID: 25683712

Dense fibrillar collagen is a potent inducer of invadopodia via a specific signaling network.
Artym,VV et al.: J Cell Biol., 208(3),331(2015).
doi: 10.1083/jcb.201405099. PMID:25646088

Identification of the PRMT1v1 and PRMT1v2 specific interactomes by quantitative mass spectrometry in breast cancer cells.
Baldwin,RM et al.: Proteomics., 15(13),2187(2015).
doi: 10.1002/pmic.201400209. PMID: 25690678

NTR1 is required for transcription elongation checkpoints at alternative exons in Arabidopsis.
Dolata, J et al.: EMBO J., 34(4),544(2015).
doi: 10.15252/embj. 201489478. PMID: 25568310

Optogenetic control of organelle transport and positioning.
van Bergeijk, P et al.: Nature, 518(7537),111(2015).
doi: 10.1038/nature14128. PMID: 25561173

■使用例：共免疫沈降 (Co-immunoprecipitation : Co-IP)

COPII coat composition is actively regulated by luminal cargo maturation.
Manzano-Lopez, J et al.: Curr Biol., 25(2),152(2015).
doi: 10.1016/j.cub.2014.11.039. PMID: 25557665

Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth.
Raiborg, C et al.: Nature, 520(7546),234(2015).
doi: 10.1038/nature14359. PMID: 25855459

Autophagic clearance of polyQ proteins mediated by ubiquitin-Atg8 adaptors of the conserved CUET protein family.
Lu, K et al.: Cell, 158(3),549(2014).
doi: 10.1016/j.cell.2014.05.048. PMID: 25042851

A new non-catalytic role for ubiquitin ligase RNF8 in unfolding higher-order chromatin structure.
Luijsterburg, MS et al.: EMBO J., 31(11),2511(2012).
doi: 10.1038/emboj.2012.104. PMID: 22531782

■クロマチン免疫沈降 (Chromatin immunoprecipitation : ChIP)

Histone Variant H2A.Z.2 Mediates Proliferation and Drug Sensitivity of Malignant Melanoma.
Vardabasso, C et al.: Mol Cell., 59(1),75(2015).
doi: 10.1016/j.molcel.2015.05.009. PMID: 26051178

■質量分析 (Mass Spectrometry)

Organization of Mitochondrial Gene Expression in Two Distinct Ribosome-Containing Assemblies.
Kehrein, K et al.: Cell Rep.(2015). pii: S2211-1247(15)00025-X.
doi: 10.1016/j.celrep.2015.01.012. PMID: 25683707

The mRNA decay factor PAT1 functions in a pathway including MAP kinase 4 and immune receptor SUMM2.
Roux, ME et al.: EMBO J.,34(5),593(2015).
doi: 10.15252/embj.201488645. PMID: 25603932

Calcium-dependent regulation of Rab activation and vesicle fusion by an intracellular P2X ion channel.
Parkinson, K et al.: Nat Cell Biol.,16(1),87(2014).
doi: 10.1038/ncb2887. PMID: 24335649