LabAssay™ Triglyceride

for Cellbiology

Manufacturer :: FUJIFILM Wako Pure Chemical Corporation

Storage Condition :: Keep at 2-10 degrees C.

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Comparison	Product Number	Package Size	Price	Inventory
	Distributor 291-94501 Barcode No 4548995103512	350Tests	Inquire	In stock in Japan	Certificate of Analysis

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350Tests

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Kit component

Kit Contents

Buffer Solution	105 mL x 1 bottle
Chromogen Substrate	for 105 mL x 1 bottle
Standard Solution	4 mL x 1 bottle

Overview

Introduction

Triglycerides are neutral fats consisting of three fatty acids esterified to a glycerol backbone. There are triglycerides, cholesterol, phospholipids, free fatty acids and fat-soluble vitamins as lipid-soluble substances in the blood.

Assay principle

Triglycerides are converted to glycerol-3-phosphate by lipoprotein lipase and glycerolkinase. Hydrogen peroxide, which is produced by a reaction between the glycerol-3-phosphate and glycerol-3-phosphate oxidase(GPO), promotes oxidative condensation of N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt(DAOS) with 4-aminoantipyrine. LabAssay™ Triglyceride can be used to detect triglycerides concentration in samples by measuring absorbance of a blue color which is generated by the oxidative condensation reaction.

Procedure

Perform the assay in the wells according to the following table scheme.

	Test	Standard	Blank
Chromogen reagent	300 μL	300 μL	300 μL
Sample	Serum 2 μL	Standard solution 2 μL	-
	Mix well and incubate at 37 ℃ for 5 min. Measure the absorbance at 600 nm^*1 of the test sample and standard solution with the blank solution as the control.

*1: In two wavelength assay, measure using Main wavelength 600 nm/Sub wavelength 700 nm.

Data

Performance

Sample	Plasma, Serum, Urine
Target animals	Human, Mouse, Rat, Cat, Dog
Standard curve range	100 - 888 mg/dL (mg/100 mL)
Measurement time	Approx. 10 min.
Amount of sample	2 μL
Measurement wavelength	600 nm(Main), 700 nm(Sub)

Standard Curve

Spike-and-Recovery Test

Spike and Recovery Test of Triglyceride, a known amount of standard is spiked into in human, mouse, rat sample n=2.

Human Sample	Spike(mg/dL)	Mean(mg/dL)	Recovery(mg/dL)	Recovery(%)
Serum	-	126	-	-
	145	267	141	97.2
	218	323	196	89.9
	291	389	263	90.4
	363	455	329	90.6
Plasma (EDTA)	-	136	-	-
	150	276	140	93.3
	225	329	193	85.8
	300	414	278	92.7
	374	460	324	86.6
Plasma (Heparin)	-	135	-	-
	150	269	134	89.3
	226	320	186	82.3
	301	394	260	86.4
	376	457	322	85.6

Rat Sample	Spike(mg/dL)	Mean(mg/dL)	Recovery(mg/dL)	Recovery(%)
Serum	-	130	-	-
	131	257	127	97.0
	196	307	177	90.3
	262	370	240	91.6
	327	413	283	86.5
Plasma (EDTA)	-	128	-	-
	135	256	128	94.8
	203	312	183	90.1
	271	374	245	90.4
	338	401	272	80.5
Plasma (Heparin)	-	123	-	-
	134	257	134	100
	201	305	182	90.5
	268	365	242	90.3
	335	400	278	83.0

Mouse Sample	Spike(mg/dL)	Mean(mg/dL)	Recovery(mg/dL)	Recovery(%)
Serum	-	133	-	-
	129	268	136	105
	194	342	209	108
	258	409	276	107
	323	448	315	97.5
Plasma (EDTA)	-	139	-	-
	129	288	149	116
	193	342	203	105
	258	409	270	105
	322	446	307	95.3
Plasma (Heparin)	-	151	-	-
	132	292	141	107
	198	349	198	100
	264	423	272	103
	329	442	291	88.4

Linearity-of-Dilution Test

Human serum
Rat serum
Mouse serum

Example of measurement

Human serum, pooled
No.	Measured(mg/dL)		Mean(mmol/L)	SD	CV(%)
1	123.5	129.4	126.4	4.18	3.30
Human plasma EDTA, pooled
No.	Measured(mg/dL)		Mean(mmol/L)	SD	CV(%)
1	137.1	135.3	136.2	1.25	0.92

Nomal Rat serum, Sprague Dawley, male
No.	Measured(mg/dL)		mean	SD	CV(%)
1	54.5	53.1	53.8	1.0	1.9
2	153	153	153	0.41	0.27
3	105	95.8	100	6.4	6.3
4	120	125	123	4.1	3.4
5	102	107	104	3.7	3.5

Nomal Rat plasma K2EDTA, Sprague Dawley, male
No.	Measured(mg/dL)		mean	SD	CV(%)
1	56.8	64.3	60.6	5.3	8.8
2	73.2	67.2	70.2	4.3	6.1
3	95.1	85.5	90.3	6.8	7.5
4	68.5	63.8	66.2	3.3	5.0
5	76.2	68.9	72.5	5.1	7.1

Nomal Mouse serum, CD-1(ICR), male
No.	Measured(mg/dL)		mean	SD	CV(%)
1	80.8	77.9	79.3	2.0	2.6
2	78.4	76.7	77.6	1.2	1.6
3	81.3	75.6	78.4	4.1	5.2
4	57.4	60.3	58.8	2.0	3.5
5	48.7	53.3	51.0	3.3	6.4

Nomal Mouse plasma K2EDTA, CD-1(ICR), male
No.	Measured(mg/dL)		mean	SD	CV(%)
1	28.8	31.7	30.2	2.0	6.7
2	132	145	139	9.5	6.9
3	62.7	68.0	65.4	3.8	5.8
4	29.5	25.8	27.6	2.6	9.5
5	58.8	55.1	56.9	2.7	4.7

Nomal Canine plasma EDTA,Beagle,male
No.	Measured(mg/dL)		mean	SD	CV(%)	Age(years)
1	37.2	33.9	35.5	2.3	6.5	4
2	29.0	27.6	28.3	1.0	3.6	6
3	32.5	36.0	34.2	2.5	7.4	10
4	33.5	36.4	34.9	2.1	5.9	12
5	33.2	30.3	31.8	2.1	6.6	6
6	24.9	22.9	23.9	1.4	6.0	11
7	31.9	28.4	30.2	2.5	8.2	10
8	63.2	57.0	60.1	4.36	7.3	12

Nomal Canine serum,Beagle,male
No.	Measured(mg/dL)		mean	SD	CV(%)	Age(years)
1	35.6	39.7	37.7	2.9	7.7	4
2	33.8	37.0	35.4	2.2	6.3	6
3	28.6	31.0	29.8	1.7	5.7	6
4	102	109	105	4.6	4.4	11
5	37.3	34.2	35.8	2.2	6.0	12
6	25.3	24.4	24.8	0.68	2.7	6
7	18.6	16.5	17.5	1.4	8.1	10
8	47.7	42.7	45.2	3.5	7.9	4

Nomal Feline plasma EDTA,Short hair,male
No.	Measured(mg/dL)		mean	SD	CV(%)	Age(years)
1	104	104	104	0.21	0.20	0.5
2	34.3	31.8	33.1	1.7	5.3	1.0
3	54.8	49.3	52.1	3.9	7.5	3.0
4	65.1	60.7	62.9	3.1	5.0	0.5
5	43.9	44.4	44.1	0.41	0.94	3.0
6	29.0	31.9	30.5	2.1	6.7	3.0
7	16.8	16.2	16.5	0.4	2.5	0.5
8	25.0	27.2	26.1	1.5	5.8	1.0

Nomal Feline serum,Short hair,male
No.	Measured(mg/dL)		mean	SD	CV(%)	Age(years)
1	32.4	35.9	34.1	2.5	7.3	3.0
2	18.4	20.2	19.3	1.3	6.7	3.0
3	33.9	36.6	35.3	1.9	5.4	6.0
4	48.9	53.8	51.3	3.5	6.8	4.0
5	33.9	32.8	33.4	0.82	2.5	6.0
6	45.3	47.1	46.2	1.24	2.7	6.0
7	96.6	88.0	92.3	6.1	6.6	1.0
8	52.3	54.7	53.5	1.7	3.1	1.0

References

Gao, F. et al. : Evid. Based Complement. Alternat. Med., 2015, 801291 (2015). *Extraction liquid of rat liver
Funakoshi, T. et al. : Biochem. Biophys. Rep., 13, 39 (2018). *Rat muscle satellite cell
Moser, V. A. and Pike, C. J. : eNeuro, 4, e0077-17 (2017). *Rat plasma
Fujii, N. et al. : Aging Cell, 16, 508 (2017). *Rat plasma
Wang, F. et al. : J. Mol. Endocrinol., 52, 133 (2014). *Rat plasma
Kato, H. et al. : J. Hepatol., 60, 1032 (2014). *Extraction liquid of mouse liver
Fujimori, K. et al. : Prostaglandins. Other. lipid. Mediat., 94, 96 (2011). *Extraction liquid of mouse culture cells

Overview / Applications

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Manufacturer Information

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632-50991 (Previous code)
LABTRIG-M1 (Previous code)
290-63701 (Previous code)

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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