LabAssay (TM) Glucose
- for Cellbiology
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
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500Tests
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In stock in Japan |
Document
Kit component
Kit component
| Buffer | 150 mL x 1 bottle |
|---|---|
| Chromogen Substrate | for 150 mL x 1 bottle |
| Standard Solution I | 3 mL x 1 bottle |
| Standard Solution II | 3 mL x 1 bottle |
Overview
Introduction
Sugar is one of the most important sources of energy in biology. It is regulated by various factors within an organism. Glucose converges to a stable ratio of α-form and β-form in solutions.
Assay principle
α-D-Glucose is converted to β-D-Glucose by mutarotase. Hydrogen peroxide, which is produced by a reaction between β-D-Glucose and glucose oxidase(GOD), promotes oxidative condensation of phenol with 4-aminoantipyrine quantitatively. LabAssay™ Glucose is a kit used for the quantitative determination of glucose concentrations in samples by measuring absorbance of a red color which is generated by the oxidative condensation reaction.
Performance
| Sample | Serum, plasma(Mouse, Rat, Human) |
|---|---|
| Standard curve range | 50 - 500 mg/dL (mg/100 mL) |
| Measurement time | approx. 10 min. |
| Amount of sample | 2 μL |
| Measurement wavelength | 505 nm(main), 600 nm(sub) |
Standard Curve
Procedure
Perform the assay in the wells according to the following table scheme.
| Test | Standard | Blank | |
|---|---|---|---|
| Chromogen reagent | 300 μL | 300 μL | 300 μL |
| Sample | 2 μL | 2 μL | - *1 |
| Mix well and incubate at 37 ℃ for 5 min. Measure the absorbance at 505 nm*2 of the test sample and standard solution with the blank solution as the control. |
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*1: The addition of water is omitted because the absorbance difference in the presence of the addition of 2 μL of water does not exhibit any practical use.
*2: Use 505 nm (Main) and 600 nm (Sub) for two wavelengths.
Data
Spike-and-Recovery Test
Spike and Recovery Test of Triglyceride, a known amount of standard is spiked into in human,mouse,rat sample n=2
| Human Sample | Spike(mg/dL) | Mean(mg/dL) | Recovery(mg/dL) | Recovery(%) |
|---|---|---|---|---|
| Serum | - | 104 | - | - |
| 109 | 205 | 101 | 92.7 | |
| 163 | 246 | 143 | 87.7 | |
| 218 | 308 | 204 | 93.6 | |
| 272 | 363 | 259 | 95.2 | |
| Plasma (EDTA) | - | 100 | - | - |
| 114 | 201 | 101 | 88.6 | |
| 171 | 249 | 149 | 87.1 | |
| 228 | 306 | 206 | 90.4 | |
| 285 | 357 | 257 | 90.2 | |
| Plasma (Heparin) | - | 103 | - | - |
| 112 | 219 | 116 | 104 | |
| 168 | 262 | 159 | 94.6 | |
| 224 | 308 | 205 | 91.5 | |
| 280 | 369 | 266 | 95.0 |
| Rat Sample | Spike(mg/dL) | Mean(mg/dL) | Recovery(mg/dL) | Recovery(%) |
|---|---|---|---|---|
| Serum | - | 96.1 | - | - |
| 86.1 | 179 | 82.5 | 95.8 | |
| 129 | 214 | 117 | 90.7 | |
| 172 | 258 | 162 | 94.2 | |
| 215 | 295 | 199 | 92.6 | |
| Plasma (EDTA) | - | 101 | - | - |
| 95.0 | 193 | 92.0 | 96.8 | |
| 143 | 226 | 125 | 87.4 | |
| 190 | 273 | 172 | 90.5 | |
| 238 | 310 | 209 | 87.8 | |
| Plasma (Heparin) | - | 103 | - | - |
| 99.0 | 196 | 93.0 | 93.9 | |
| 149 | 226 | 123 | 82.6 | |
| 198 | 279 | 176 | 88.9 | |
| 248 | 319 | 216 | 87.1 |
| Mouse Sample | Spike(mg/dL) | Mean(mg/dL) | Recovery(mg/dL) | Recovery(%) |
|---|---|---|---|---|
| Serum | - | 98.1 | - | - |
| 89.2 | 177 | 78.9 | 88.5 | |
| 134 | 201 | 103 | 76.9 | |
| 178 | 251 | 153 | 86.0 | |
| 223 | 280 | 182 | 81.6 | |
| Plasma (EDTA) | - | 93 | - | - |
| 90.8 | 174 | 81.0 | 89.2 | |
| 136 | 203 | 110 | 80.9 | |
| 182 | 248 | 155 | 85.2 | |
| 227 | 276 | 183 | 80.6 | |
| Plasma (Heparin) | - | 111 | - | - |
| 91.2 | 187 | 76.0 | 83.3 | |
| 137 | 227 | 116 | 84.7 | |
| 182 | 263 | 152 | 83.5 | |
| 228 | 294 | 183 | 80.3 |
Linearity-of-Dilution Test
- Human serum
- Rat serum
- Mouse serum
Example of measurement
| Human serum, pooled | |||||
|---|---|---|---|---|---|
| No. | Measured(mg/dL) | Mean(mmol/L) | SD | CV(%) | |
| 1 | 104.2 | 102.9 | 103.6 | 0.97 | 0.94 |
| Human plasma EDTA, pooled | |||||
| No. | Measured(mg/dL) | Mean(mmol/L) | SD | CV(%) | |
| 1 | 99.8 | 100.2 | 100.0 | 0.00 | 0.00 |
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Nomal Rat serum, Sprague Dawley, male No. Measured(mg/dL) mean SD CV(%) 1 132.2 139.9 136.1 5.4 4.0 2 113.9 122.3 118.1 5.9 5.0 3 135.2 143.8 139.5 6.1 4.3 4 118.4 114.9 116.6 2.5 2.2 5 132.0 136.9 134.5 3.5 2.6 -
Nomal Rat plasma K2EDTA, Sprague Dawley, male No. Measured(mg/dL) mean SD CV(%) 1 107.3 108.2 107.8 0.64 0.59 2 108.9 112.5 110.7 2.6 2.3 3 124.3 123.4 123.9 0.65 0.52 4 105.0 111.2 108.1 4.4 4.1 5 110.2 118.5 114.3 5.9 5.1
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Nomal Mouse serum, CD-1(ICR), male No. Measured(mg/dL) mean SD CV(%) 1 127.1 132.6 129.9 3.9 3.0 2 145.9 148.1 147.0 1.5 1.0 3 143.4 158.4 150.9 11 7.0 4 150.2 157.1 153.6 4.9 3.2 5 136.3 132.2 134.3 2.9 2.1 -
Nomal Mouse plasma K2EDTA, CD-1(ICR), male No. Measured(mg/dL) mean SD CV(%) 1 86.8 87.9 87.3 0.72 0.83 2 88.3 94.4 91.3 4.4 4.8 3 100.2 95.1 97.7 3.6 3.7 4 107.7 98.8 103.2 6.3 6.1 5 106.1 108.6 107.3 1.8 1.6
| Nomal Mouse Whole Blood K2EDTA, CD(ICR), male | |||||
|---|---|---|---|---|---|
| No. | Measured(mg/dL) | mean | SD | CV(%) | |
| 1 | 81.6 | 82.9 | 82.2 | 0.91 | 1.1 |
| 2 | 108 | 118 | 113 | 7.1 | 6.2 |
| 3 | 92.0 | 84.5 | 88.2 | 5.3 | 6.0 |
| 4 | 95.5 | 101 | 98.2 | 3.8 | 3.9 |
| 5 | 69.3 | 67.2 | 68.3 | 1.5 | 2.2 |
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Nomal Canine serum,Beagle,male No. Measured(mg/dL) mean SD CV(%) Age(years) 1 97.6 110 104 9.1 8.7 4 2 71.1 79.7 75.4 6.1 8.1 6 3 65.2 75.0 70.1 6.9 9.8 6 4 63.3 62.7 63.0 0.39 0.61 11 5 77.6 86.5 82.0 6.3 7.7 12 6 73.3 79.0 76.2 4.0 5.3 6 7 36.8 38.8 37.8 1.4 3.8 10 8 56.2 60.2 58.2 2.8 4.9 4 -
Nomal Canine plasma EDTA,Beagle,male No. Measured(mg/dL) mean SD CV(%) Age(years) 1 97.4 89.3 93.4 5.7 6.1 4 2 96.0 90.9 93.5 3.6 3.9 6 3 88.5 81.4 84.9 5.0 5.9 10 4 78.6 71.8 75.2 4.8 6.4 12 5 85.5 80.2 82.9 3.8 4.5 6 6 75.0 71.8 73.4 2.2 3.0 11 7 85.7 83.7 84.7 1.4 1.6 10 8 83.9 82.6 83.2 0.91 1.1 12
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Nomal Feline serum, Short hair, male No. Measured(mg/dL) mean SD CV(%) Age(years) 1 63.2 64.7 64.0 1.1 1.7 3.0 2 65.2 67.1 66.1 1.3 2.0 3.0 3 32.4 37.0 34.7 3.2 9.4 6.0 4 40.2 43.3 41.8 2.2 5.3 4.0 5 44.8 44.2 44.5 0.46 1.0 6.0 6 21.4 20.3 20.8 0.72 3.4 6.0 7 253 248 250 3.7 1.5 1.0 8 98.2 91.6 94.9 4.7 5.0 1.0 -
Nomal Feline plasma EDTA, Short hair, male No. Measured(mg/dL) mean SD CV(%) Age(years) 1 200 201 200 0.94 0.47 0.5 2 118 115 117 2.3 2.0 1.0 3 223 224 224 0.54 0.24 3.0 4 272 261 266 7.5 2.8 0.5 5 120 119 119 0.83 0.69 3.0 6 115 112 114 2.0 1.8 3.0 7 134 136 135 1.4 1.0 0.5 8 107 109 108 1.5 1.4 1.0
References
- Yamashita, Y. et al. : Biosci. Biotechnol. Biochem., 77, 888 (2013). *Mouse plasma
- Narita, T. et al. : Exp. Gerontol., 104, 127 (2018). *Rat plasma
- Yamasaki, M. et al. : Food Sci. Technol. Res., 21, 827 (2015). *Mouse serum
- Fan, Y. et al. : J. Biomed. Sci., 23, 56 (2016). *Mouse serum
- Wang, W. et al. : J. Renin Angiotensin Aldosterone Syst., 15, 384 (2014). *Mouse serum
- Yamashita, Y. et al. : J. Nutr. Sci., 1, e2 (2012). *Mouse plasma
- Maesako, M. et al. : Neurobiol. Aging., 33, 1011 (2012). *Mouse serum
Overview / Applications
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Manufacturer Information
Alias
- 638-50971 (Previous Code)
LABGLUC-M1 (Previous Code)
298-65701 (Previous Code)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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