Tau ELISA Kit Wako
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Comparison
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Product Number
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96Tests
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In stock in Japan |
Document
Kit component
For 96 tests
| Antibody-coated Plate | 1 plate, 96 wells ( 8 x 12) |
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| Tau Standard | 1 tube |
| Buffer | 60 mL |
| Biotin-conjugated Antibody Solution | 100 µL |
| Peroxidase-conjugated Streptavidin Solution | 100 µL |
| Luminescent Reagent 1 | 6 mL |
| Luminescent Reagent 2 | 6 mL |
| Wash Solution (10x) | 100 mL |
| Sample Buffer 1 | 0.6 mL |
| Sample Buffer 2 | 30 mL |
| Plate Seal | 3 sheets |
Overview/ Performance
Tau is a microtubule-associated protein. It is mainly expressed in neurons of the central nervous system and regulates the stability of microtubules. In the brain of patients with Alzheimer’s disease, phosphorylated Tau aggregates and forms neurofibrillary tangles (NFTs). There is evidence suggesting that the extent of NFTs correlates with the severity of dementia. Therefore, Tau has been studied extensively to better understand the causes of Alzheimer’s disease and to develop novel therapeutics.
Studies suggest that the concentration of Tau and phosphorylated Tau in the cerebrospinal fluid is elevated in patients with Alzheimer’s disease compared with non-dementia patients.
The kit facilitates the measurement of Tau, and recognizes any Tau regardless of the state of phosphorylation.
Performance
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Standard curve range 4.10 pg/mL - 1,000 pg/mL Reactive Tau Total Tau Sample Human cerebrospinal fluid (CSF) Sample volume 10 µL～※1 Assay time 3 hours Detection method Luminescent system※2 ※1: It is recommended that CSF sample volume is 50 μL, considering the accuracy of dilutions.
※2: A luminescence plate reader is required for the assay.
Principles
It is a sandwich ELISA assay that combines the use of antibodies that recognize the sequences of the middle and C-terminal region of Tau.
It recognizes all types of Tau.
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Tau isoforms
Phosphorylation sites of Tau
Application data
Spike-and-recovery and dilution linearity
Spike-and-recovery
| Human CSF 1 | Not spiked | + 20 pg/mL Tau | + 60 pg/mL Tau | + 150 pg/mL Tau | |||
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| Measured | Measured | Recovery | Measured | Recovery | Measured | Recovery | |
| 4.09 pg/mL | 24.8 pg/mL | 104％ | 58.1 pg/mL | 90％ | 175 pg/mL | 114％ | |
| Human CSF 2 | Not spiked | + 40 pg/mL Tau | + 135 pg/mL Tau | + 380 pg/mL Tau | |||
| Measured | Measured | Recovery | Measured | Recovery | Measured | Recovery | |
| 24.8 pg/mL | 70.5 pg/mL | 114％ | 160 pg/mL | 100％ | 390 pg/mL | 96.1％ | |
Dilution linearity
These assays demonstrated that the kit is highly quantitative.
Measurements of human CSF
CSF collected from non-dementia, mild cognitive impairment (MCI), and Alzheimer’s disease (AD) patients were analyzed with our ELISA kit and a competitor’s product.
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The differences between non-dementia, MCI, and AD groups were more significant when measured with our kit.
Correlation with Mini Mental State Examination (MMSE) scores
The correlation between MMSE scores and the concentration of Tau was examined. Samples were collected from CSF and measured using our ELISA kit.
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※MMSE score
≤23: suspected of dementia
24-27: suspected of mild cognitive impairment
28-30: normal
There was a correlation between the concentration of Tau and MMSE score. The correlation was stronger when Tau concentration was measured using our product.
Overview / Applications
| Outline | This product is for research use only. Do not administer it to human.Tau is one of the microtubule-associated proteins, which is mainly expressed in neurons of the central nervous system, and controls the stabilization of microtubules. In the brains of Alzheimer's disease patients, it is reported that neurofibrillary tangle accumulated by phosphorylated Tau is formed, and the extent of its appearance correlates with the severity of dementia. Therefore, Tau is being studied for investigating the cause of Alzheimer's disease and developing therapeutic drugs. This product is an ELISA kit which can easily measure Tau and recognizes all Tau regardless of phosphorylation state. [Calibration curve range] 4.1-1000 pg/mL [Measurement sample] human cerebrospinal fluid [Required amount of specimen] 10 μL - [Measurement time] 3 hours [Detection method] Light emitting system |
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Property
Manufacturer Information
Alias
- Total Tau ELISA Kit
MAPT ELISA Kit
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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