Silver Stain MS Kit

for Electrophoresis

Manufacturer :: FUJIFILM Wako Pure Chemical Corporation

Storage Condition :: Keep at 2-10 degrees C. (RT)

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	Distributor 299-58901 Barcode No 4987481416901	20Tests	Inquire	In stock in Japan	Certificate of Analysis

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Outline	This product is for research use only. Do not administer it to human. This silver staining kit is produced for mass spectrometric sequencing of protein separated by polyacrylamide gel electrophoresis, based on the method described by Shevchenko, et al. By silver staining, protein is rarely modified chemically due to omitting treatment of glutaraldehyde and is detected at several nanograms level on the elecrophoretic gel. [Features] Detectable limit of protein: 1 ng ideal for MS analysis because rarely modified protein is obtained due to omitting glutaraldehyde treatment. Procedure[SDS-PAGE gel] Fixing Soln. 1 Shake 20 minutes Fixing Soln. 2 Shake 10 minutes and wash 10 minutes. Enhancing Soln. Shake 1 minute and wash 2 x 1 minute. Staining Soln. Shake 20 minutes and wash 2 x 1 minute. Developing Soln. Shake 3 ~ 10 minutes. Stopper Shake 1 minute and wash 3 x 1 minute. *[The excised band* De-staining mixture Leave 15 minutes. *[Mass Spectrometry]* [Kit Contents] Enhancing Stock Soln. 1 bottle x 200 mL Staining Stock Soln. 1 bottle x 200 mL Developing Stock Soln. 1 bottle x 100 mL Developing Powder 1 bottle x 20 g Stopper 1 bottle x 200 mL De-staining Soln.A 1 bottle x 50 mL De-staining Soln.B 1 bottle x 50 mL [References] Shevchenko,A., et al.:Anal.Chem. 68, 850 (1996) Gharahdaghi,F., et al.:Electrophoresis, 20, 601 (1999)

Outline

This product is for research use only. Do not administer it to human.

This silver staining kit is produced for mass spectrometric sequencing of protein separated by polyacrylamide gel electrophoresis, based on the method described by Shevchenko, et al. By silver staining, protein is rarely modified chemically due to omitting treatment of glutaraldehyde and is detected at several nanograms level on the elecrophoretic gel.

[Features]

Detectable limit of protein: 1 ng
ideal for MS analysis because rarely modified protein is obtained due to omitting glutaraldehyde treatment.

Procedure[SDS-PAGE gel]

Fixing Soln. 1
Fixing Soln. 2
Enhancing Soln.
Staining Soln.
Developing Soln.
Stopper

[The excised band

De-staining mixture

[Mass Spectrometry]

[Kit Contents]

Enhancing Stock Soln. 1 bottle x 200 mL
Staining Stock Soln. 1 bottle x 200 mL
Developing Stock Soln. 1 bottle x 100 mL
Developing Powder 1 bottle x 20 g
Stopper 1 bottle x 200 mL
De-staining Soln.A 1 bottle x 50 mL
De-staining Soln.B 1 bottle x 50 mL

[References]

Shevchenko,A., et al.:Anal.Chem. 68, 850 (1996)
Gharahdaghi,F., et al.:Electrophoresis, 20, 601 (1999)

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Silver Stain 2 Kit wako	for Electrophoresis

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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