SARS-CoV-2 Real-Time RT-PCR Detection Kit(N gene)
- for Genetic Research
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
- Structural Formula
- Label
- Packing
- SDS
Document
Kit component
Kit components (for 200 reactions)
| 5×Reaction Buffer | 800 μL |
|---|---|
| 2mmol/L dNTPs | 800 μL |
| 50mmol/L Manganese(II) Acetate | 200 μL |
| Hot Start Reverse Transcription DNA Polymerase | 50 μL |
| Distilled Water, Nuclease-free | 590 μL |
| Fw and Rv Primers 1 | 100 μL |
| TaqManⓇ Probe 1 | 40 μL |
| Fw and Rv Primers 2 | 100 μL |
| TaqManⓇ Probe 2 | 40 μL |
| Positive Control RNA, N gene | 1,000 μL |
| *A ROX for passive reference dye is not supplied in this kit. Please prepare it as needed. TaqManⓇ is registered trademark by Roche Diagnostics K.K. | - |
Materials required but not provided
| Real-Time PCR instrument | - |
|---|---|
| Microcentrifuge | - |
| Micro pipette and nuclease-free pipette tips | - |
| Nuclease-free 1.5mL tubes(e.g. Micro tube 1.5ml DNA LowBind, Sarstedt） | - |
| Real-time PCR plates and plate films or real-time PCR tubes and caps. | - |
| TaqMan® is registered trademark by Roche Diagnostics K.K. | - |
Overview
Product description
SARS-CoV-2 Real-Time RT-PCR Detection Kits a reagent system, based on an one-step real-time PCR method, for the qualitative detection of SARS-CoV-2-derived RNA. The highly active enzyme applied in the kit allows high-speed real-time PCR and it plays dual roles as reverse transcriptase and DNA polymerase.
The primer/probe No.1 and 2 are designed to hybridize to N(Nucleocapsid) gene and labeled with the FAM.
Key Features
- RT-PCR only for 45 minutes
- Inhibiting non-specific amplification by hot start PCR
- Including positive controls
Two targets at N gene
Protocol flow
-
- Prepare extracted RNA from SARS-CoV-2.
- Dilute Positive Control RNA with water.
- Prepare PCR master mix.
- Apply PCR master mix and RNA into real-time PCR plate or tube.
- Run a real-time PCR instrument.
Probe Reporter TaqMan® Probe 1 FAM TaqMan® Probe 2 FAM
Example of arrangement of applying samples in 96-well plate
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9-12 | |
| A | |||||||||
| B | P | S1 & No.1 | S2 & No.1 | S3 & No.1 | S4 & No.1 | S5 & No.1 | |||
| C | N | S1 & No.2 | S2 & No.2 | S3 & No.2 | S4 & No.2 | S5 & No.2 | |||
| D | |||||||||
| E | |||||||||
| F | |||||||||
| G | |||||||||
| H |
S：Sample
No.1：Fw & Rv Primers 1, TaqMan® Probe 1
No.2：Fw & Rv Primers 2, TaqMan® Probe 2
P : Positive control
N : Negative control
| Two wells are used for one sample |
Example applications
Positive control RNA, N=2
Positive control RNA, N=2
Standard Curve
Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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