Trypsin, from Porcine Pancreas, Mass Spectrometry Grade

[BIOCHEMISTRY]

For Proteome Research!

Among the most important techniques in proteome analyses is the in-gel digestion of protein spots/bands that have been resolved by electrophoresis using digestive enzymes, such as trypsin and lysyl endopoptidase. Proteins can be identified by mass spectrometry analysis of the peptides produced by in-gel digestion, and further information regarding post-translational modifications can be obtained.Lysyl Endopeptidase, Mass Spectrometry Grade is a freeze dried product that retained sufficient activity for in-gel digestion and packed in very small quantities for convenience purposes.

[Features]

1. High specificity and efficiency of protein digestion allow for easy database searches by peptide mass.
2. Improved cleavage at lysine residue and increase in the number of peptides are obtained by combination with trypsin.
3. Packed in very small quantities according to the amounts used so that sufficient activity for in-gel digestion may be retained.

• Wako Catalog #293-57701 Negative Gel Stain MS Kit, for Electrophoresis (20 tests)
• Wako Catalog #299-58901 Silver Gel Stain MS Kit, for Electrophoresis (20 tests)

1. Wada, Y., and Kadoya, M.: J. Mass Spectrom., 38, 117 (2003).
2. Shevchenko, A., Wilm, MM., Vorm, O., and Mann., M.: Anal. Chem., 68, 850 (1996).