Ninhydrin

for Amino Acid Automated Analysis

Manufacturer :: FUJIFILM Wako Pure Chemical Corporation

Storage Condition :: Keep at RT.

CAS RN^® :: 485-47-2
Molecular Formula :: C9H6O4
Molecular Weight :: 178.14

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Structural Formula
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SDS

Product Number	Package Size	Price	Inventory
Distributor 209-02771 Barcode No 4987481335837	1G	Inquire	In stock in Japan	Certificate of Analysis
Distributor 203-02774 Barcode No 4987481335868	1G x10	Inquire	In stock in Japan	Certificate of Analysis
Distributor 207-02772 Barcode No 4987481335844	25G	Inquire	In stock in Japan	Certificate of Analysis
Distributor 205-02773 Barcode No 4987481335851	100G	Inquire	In stock in Japan	Certificate of Analysis
Distributor 201-02775 Barcode No 4987481335875	500G	Inquire	In stock in Japan	Certificate of Analysis

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Product Specification Sheet

Spectral Data

Certificate of Analysis

Calibration Certificate

Overview / Applications

Outline	Amino Acid Analysis Reagents are high-purity reagents from which ninhydrin positive components that would cause interference in the analysis have been removed.Ninhydrin reacts with amino acids and yields the bluish purple Ruhemann's purple at around pH 5. For this reaction, reduced ninhydrin needs to be formed first. Therefore, reducing agents such as tin chloride or titanium chloride are added to the ninhydrin reagent for quantitative analysis of amino acids, or reduced ninhydrin (hydrintantin) is employed with the reagent. Methyl cellosolve is usually used as the solvent for ninhydrin reagent, however, as methyl cellosolve is toxic, it is sometimes replaced by dimethyl sulfoxide. As ninhydrin solution is easily oxidated in the air, it is prepared under nitrogen.Ninhydrin reagent is said to be stable for about 2 weeks. However, since its color development decreases gradually with time, a certain amount of an amino acid other than the constituent amino acids in the analyte protein should be added as an internal standard, and the analytical value should be corrected using the recovery rate of the internal standard. The detection limit is 100 pmol maximum, and 250 pmol for proline.

Outline

Amino Acid Analysis Reagents are high-purity reagents from which ninhydrin positive components that would cause interference in the analysis have been removed.Ninhydrin reacts with amino acids and yields the bluish purple Ruhemann's purple at around pH 5. For this reaction, reduced ninhydrin needs to be formed first. Therefore, reducing agents such as tin chloride or titanium chloride are added to the ninhydrin reagent for quantitative analysis of amino acids, or reduced ninhydrin (hydrintantin) is employed with the reagent. Methyl cellosolve is usually used as the solvent for ninhydrin reagent, however, as methyl cellosolve is toxic, it is sometimes replaced by dimethyl sulfoxide. As ninhydrin solution is easily oxidated in the air, it is prepared under nitrogen.Ninhydrin reagent is said to be stable for about 2 weeks. However, since its color development decreases gradually with time, a certain amount of an amino acid other than the constituent amino acids in the analyte protein should be added as an internal standard, and the analytical value should be corrected using the recovery rate of the internal standard. The detection limit is 100 pmol maximum, and 250 pmol for proline.

Property

Appearance	White - pale yellow, crystals - crystalline powder
Melting Point	about 240 degrees C (dec.)
Specific Gravity	0.788

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For research use or further manufacturing use only. Not for use in diagnostic procedures.

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