CultureSureTM CEPT Cocktail(1,000×)
- for Cell Culture
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
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300uL
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FAQ
APPLICATION & PROTOCOL
- What kinds of organoids have been produced?
- Organoids for brain, gut, and kidney.
- What is the impact of CEPT Cocktail on 3D culturing?
- Optimization of cell aggregation and survival
- Improvement of reproducibility
- Stress suppression during cell differentiation
- Improvement of three-dimensional structure and self-organization
- At what time is is the CEPT Cocktail added to the solution during passaging of iPSC?
- During the first 24 hours of passaging.
- At what time is the CEPT Cocktail added to the solution during freezing and thawing of iPSC?
- CEPT Cocktail should be added to the cryopreservation solution and frozen.
After thawing and cell seeding, cells should be cultured in medium with CEPT Cocktail for the first 24 hours.
- What should I check if I want more information?
- Please review the following papers:
- Chen, Y. et al. : Nat. Methods, 18(5), 528 (2021).
A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells. - Tristan, C.A. et al. : Stem Cell Reports, 16(12), 3076 (2021).
Robotic high-throughput biomanufacturing and functional differentiation of human pluripotent stem cells. - Tristan, C.A. et al. : Nat. Protoc., 18(1), 58 (2022).
Efficient and safe single-cell cloning of human pluripotent stem cells using the CEPT cocktail. - Deng, T. et al. : Stem Cell Reports, 18(4), 1030 (2023).
Scalable generation of sensory neurons from human pluripotent stem cells. - Takeshi, W. et al. : Regen. Med., 18(3), 219 (2023).
International evaluation study of a highly efficient culture assay for detection of residual human pluripotent stem cells in cell therapies.
- Chen, Y. et al. : Nat. Methods, 18(5), 528 (2021).
PRODUCTION
- Is the solution animal-free?
- Yes it is. Please contact us if you wish to issue a certificate.
Overview / Applications
| Outline | CEPT is a cocktail of small molecules developed at the National Institutes of Health (NIH). In human ES cells and human iPS cells (human pluripotent stem cells [hPSCs]), CEPT prevents cellular stress and DNA damage during cell passage. Like Y-27632, a known potent ROCK inhibitor, it improves the viability of hPSCs during cell passage and cryopreservation. Compared to existing methods, CEPT also improves cell viability in stem cell research, including embryoid body and organoid formation, single cell cloning, and genome editing using hPSCs. *This product is manufactured and sold under license from NIH. |
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Property
| Purity | Bacterial endotoxins : <3EU/mL |
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Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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