5-Carboxyfluorescein

for Cellbiology

Specification Assay :: 94.0+% (HPLC)

Manufacturer :: FUJIFILM Wako Pure Chemical Corporation

Storage Condition :: Keep at -20 degrees C.

CAS RN^® :: 76823-03-5
Molecular Formula :: C21H12O7
Molecular Weight :: 376.32

Structural Formula
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Comparison	Product Number	Package Size	Price	Inventory
	Distributor 039-19341 Barcode No 4987481436183	100mg	Inquire	Inquire	Certificate of Analysis

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Overview / Applications

Outline	This is a green fluorescence reagent used for labeling peptides, proteins and nucleic acids. Since the fluorescence intensity of this product is pH-dependent, it can be used to examine pH changes inside cells apart from a labeling reagent. Excitation wavelength: 492 nm, Emission wavelength: 518 nm This product is for research use only. Do not administer it to human. *Cellbiology* Fluorescent Reagent for in situ labeling peptides, proteins and nucleotides 5-FAM is a single isomer. It is one of the most popular green fluorescent reagents used for in situ labeling peptides, proteins and nucleotides. It has also been used to prepare various small fluorescent molecules. Fluorescence Excitation wavelength = 492 nm (pH > 7.0) Emission wavelength = 518 nm (pH > 7.0) Solvents for stock solutions: DMSO or DMF [References] Hahn, M., et al.: Electrophoresis, 22, 2691 (2001). Avrahami, D., et al.: Biochemistry, 40, 12591 (2001). Hung, S. C., et al.: Anal. Biochem., 243, 15 (1996). The product information is shown on the page #4 of Wako Product Update No.15.

Outline

This is a green fluorescence reagent used for labeling peptides, proteins and nucleic acids.
Since the fluorescence intensity of this product is pH-dependent, it can be used to examine pH changes inside cells apart from a labeling reagent.
Excitation wavelength: 492 nm, Emission wavelength: 518 nm
This product is for research use only. Do not administer it to human.

Cellbiology
Fluorescent Reagent
for in situ labeling peptides, proteins and nucleotides
5-FAM is a single isomer. It is one of the most popular green fluorescent reagents used for in situ labeling peptides, proteins and nucleotides. It has also been used to prepare various small fluorescent molecules.
Fluorescence

Excitation wavelength = 492 nm (pH > 7.0)

Emission wavelength = 518 nm (pH > 7.0)

Solvents for stock solutions: DMSO or DMF
[References]

Hahn, M., et al.: Electrophoresis, 22, 2691 (2001).

Avrahami, D., et al.: Biochemistry, 40, 12591 (2001).

Hung, S. C., et al.: Anal. Biochem., 243, 15 (1996).

The product information is shown on the page #4 of Wako Product Update No.15.

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Appearance	Reddish yellow - yellowish red, crystals - powder

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For research use or further manufacturing use only. Not for use in diagnostic procedures.

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