MildformR 10N

BIOCHEMISTRY

Histochemistry

Formalin fixative for pathological tissues, deodorizedMildform(R) productsMildform(R) products, fixatives for pathological tissues, are neutral buffered formalin solution prepared according to Lillie's formulation to which wine extract^* is added. The wine extract minimizes the irritating and unpleasant odor of formalin, however, with regard to operational safety, minimum degree of odor is present so that formalin can be recognized.

*Mechanism of wine extract's effect : The masking effect minimizes the irritating and unpleasant odor of formalin.

[Features]

1. Most suitable for tissue fixation of immunohistochemical staining by an immunoenzymatic technique.
2. The fixation and osmosis are equivalent to that of neutral buffered formalin solution or greater.

• Concentration of formaldehyde solution : 10%
• Formaldehyde Solution : 100mL
• NaH₂PO₄ ^. 2H₂O : 4.5g
• Na₂HPO₄ : 6.5g
• Methanol : -
• Water : Add water to make the 1L solution.
• Formaldehyde Content : 4%
• pH : 7.0~7.5

• Mildform(R)10N^** (Wako Catalog#133-10311)
• Mildform(R)10NM^*** (Wako Catalog#132-10521)
• Mildform(R)15N (Wako Catalog#132-14301)
• Mildform(R)15NM (Wako Catalog#139-14311)
• Mildform(R)20N (Wako Catalog#136-10041)
• Mildform(R)20NM (Wako Catalog#139-10531)

In general, NM-type is most suitable for rapid fixation with minimum length of time needed. Also suitable as a formalin-methanol fixative for fixing samples containing a large amount of connective tissues and adipose tissues.

Embedded Medium for Mouse and Rat tissues

• Wako Catalog# 167-20501 Pathoprep(R)546, (2kg x 3)
• Wako Catalog# 162-18961 Pathoprep(R)568, (500g x 12)
• Wako Catalog# 165-19551 Pathoprep(R)580, (2kg x 3)