Anti Segatella, Monoclonal Antibody (SC-L10B5-35K1)
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
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50uL
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In stock in Japan |
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Product Overview
Developed by Dr. Jun Kunisawa and Dr. Ken Yoshii at the National Institutes of Biomedical Innovation, Health and Nutrition, Fujifilm Wako now offers monoclonal antibody against Segatella copri1) (formerly Prevotella copri). It is suitable for use in ELISA, flow cytometry, immunoprecipitation, and Western blotting, allowing for rapid, simple, and cost-effective detection of gut bacteria.
Antibody Information
| Clonality | Monoclonal |
|---|---|
| Antigen | Heat-treated Segatella copri |
| Host | Mouse |
| Formulation | 1 x D-PBS, 0.05% Sodium Azide |
| Conjugate | Unlabeled |
| Cross-reactivity | Segatella copri (GGGtGRT protein) |
| Application | ELISA (Direct/Sandwich) Flow Cytometry Immunoprecipitation Western Blotting |
Data
ELISA
100 μL of a bacterial suspension was added to a 96-well microplate to coat the wells. The suspension was prepared either by diluting freeze-dried, heat-inactivated bacteria in PBS or by suspending them in B-PER solution followed by lysis with glass beads. After blocking and washing the antigen-coated plate, culture supernatants from individual hybridoma clones were added and incubated for 2 hours. Antibodies against gut bacteria bound to the antigens were detected using HRP-conjugated goat anti-mouse IgG1).
- P. vulgatus
- F. duncaniae
- S. copri
- B. pseudocatenulatum
- B. longum
- B. wexlerae
- A. muciniphila
Flow Cytometry
After fixation with Farmer’s fixative (ethanol:acetic acid = 7:3), the bacteria were centrifuged at 13,040 x g for 2 minutes at 4 °C. Next, 100 μL of the bacterial suspension was mixed with 1 μg of anti-gut bacterial antibody in PBS-T containing 1% BSA and incubated on ice for 1 hour. After washing, FITC-conjugated anti-mouse IgG was added, and the sample was incubated for 30 minutes on ice in the dark. The stained bacteria were then washed and analyzed by flow cytometry1).
Anti-S. copri
Western Blotting
Segatella copri JCM13464 was collected by centrifugation at 10,000 x g for 5 minutes. Two micrograms of protein were separated by SDS-PAGE, transferred to a PVDF membrane, and blocked. Immunodetection was performed using 25–50 ng/mL of anti-gut bacterial antibody and HRP-conjugated mouse IgG1).
References
- Yoshii, K. et al.: Sci. Rep., 15(1), 1(2025).
Establishment of enterotype-specific antibodies for various diagnostic systems
FAQ
About antibody
- Does this product react with Segatella species other than Segatella copri?
- Cross-reactivity has only been confirmed for Segatella copri used in the immune response; cross-reactivity with other bacterial species within the same genus remains unknown.
Overview / Applications
Property
Manufacturer Information
Alias
- Anti Prevotella, Monoclonal Antibody (SC-L10B5-35K1)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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