BAMBANKER™

Manufacturer :: GC LYMPHOTEC

Storage Condition :: Keep at 2-10 degrees C.

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Comparison	Product Number	Package Size	Price	Inventory
	Distributor 306-14684 Manufacturer CS-04-001 Barcode No 4987481589278	20mL x5	List Price JPY 14,400	In stock in Japan

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Overview / Applications

Outline	*Serum-Free Cell Freezing MediumBAMBANKER is a serum-free medium for long-term freezing at -80 degrees C and preservation of valuable culture cells such as tumor cells and normal cells Features* Ready-to-use medium for preservation of cells Usable without diluting No program freezer is required Rapid and long-term freezing and preservation in a deep freezer (-80 C) Serum-free Operating Procedure Centrifuge the cells in logarithmic growth phase, and collect 5 x 10^5 ~ 1 x 10^7 cells. Suspend the collected cells in 1 mL of this medium, and place the cells in cryotubes for freezing and preservation. Then, freeze and preserve the cells at -80 C without preliminary freezing. The cells frozen at -80 C can be preserved subsequently in liquid nitrogen. Thawing must be carried out quickly in a constant-temperature chamber or a bath. Sterility Test*** Endotoxin: chromogenic substrate method Mycoplasma: Fluorescent antibody method Fungi and bacteria: As per Japanese Pharmacopoeia Note* : The cells must be frozen in logarithmic growth phase.: Certificate of analysis will be issued upon request. Applications** [Cells preserved well by cryopreservation test]: P3U1 (mouse myeloma cell line), K562 (human leukemia cell line), human gastric epithelial cells, etc. Daudi (human B cell line), PC12 (rat-derived adrenal pheochromocytoma), human B cell line OKT4 (mouse hybridoma), monkey B cell line, Activated lymphocyte derived from human peripheral blood, Activated lymphocyte derived from mouse spleen Manufactured by LYMPHOTEC Inc. (Japan).

Outline

Serum-Free Cell Freezing MediumBAMBANKER is a serum-free medium for long-term freezing at -80 degrees C and preservation of valuable culture cells such as tumor cells and normal cells

Features

Ready-to-use medium for preservation of cells
Usable without diluting
No program freezer is required
Rapid and long-term freezing and preservation in a deep freezer (-80 C)
Serum-free

Operating Procedure

Centrifuge the cells in logarithmic growth phase*, and collect 5 x 10^5 ~ 1 x 10^7 cells.
Suspend the collected cells in 1 mL of this medium, and place the cells in cryotubes for freezing and preservation. Then, freeze and preserve the cells at -80 C without preliminary freezing. The cells frozen at -80 C can be preserved subsequently in liquid nitrogen.
Thawing must be carried out quickly in a constant-temperature chamber or a bath.

Sterility Test**

Endotoxin: chromogenic substrate method
Mycoplasma: Fluorescent antibody method
Fungi and bacteria: As per Japanese Pharmacopoeia

Note* : The cells must be frozen in logarithmic growth phase.**: Certificate of analysis will be issued upon request.

Applications [Cells preserved well by cryopreservation test]:

P3U1 (mouse myeloma cell line), K562 (human leukemia cell line), human gastric epithelial cells, etc.
Daudi (human B cell line), PC12 (rat-derived adrenal pheochromocytoma), human B cell line
OKT4 (mouse hybridoma), monkey B cell line, Activated lymphocyte derived from human peripheral blood, Activated lymphocyte derived from mouse spleen

Manufactured by LYMPHOTEC Inc. (Japan).

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BAMBANKER™
BAMBANKER™ Direct

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