ISOSPIN Cell & Tissue RNA
- Manufacturer :
- Nippon Gene Co., Ltd.
- Storage Condition :
- Keep at -20 degrees C.
- Structural Formula
- Label
- Packing
|
Comparison
|
Product Number
|
Package Size
|
Price
|
Inventory
|
|
|---|---|---|---|---|---|
|
|
|
50Tests
|
|
|
|
|
|
|
200Tests(50Testsx4)
|
|
|
Please check here for notes on products and prices.
Document
- Package Insert
-
- Spectral Data
-
- Certificate of Analysis
-
- Calibration Certificate
-
- Analytical Charts
-
Kit Components
| Component (50 preps) | |
|---|---|
| PT Extraction Buffer (for tissues) | 30mL x 1 |
| C Extraction Buffer (for cells) | 30mL x 1 |
| PT Binding Buffer (for tissues & cells) | 40mL x 1 |
| PT Wash1 Buffer | 40mL x 1 |
| PT Wash2 Buffer | 40mL x 1 |
| DNase I (RNase free) | 2,000 units x 1 |
| 10xDNase I Buffer | 1mL x 1 |
| ddWater (RNase free) | 1mL x 8 |
| Spin Column (a Spin Column and Collection Tube set) | 50 sets |
The kit box contains items stored at room temperature. Upon receipt, store DNase I and 10 x DNase I Buffer at -20°C.
The 200 preps package consists of 4 sets of the 50 preps kit.
Description
ISOSPIN Cell & Tissue RNA is for RNA extraction and purification kit from animal cultured cells and animal tissue.
It purifies RNA by binding it to a silica membrane in the presence of chaotropic ions, eliminating the need for phenol or chloroform. The spin columns are designed with a large column volume and a silica membrane that binds sufficient RNA, enabling the extraction and purification of high-purity RNA within one hour.
Product features
- High purity RNA extraction
- No pre-filtering required – complete extraction in 1 hour
- DNase I included
- No phenol or chloroform required
Application Data
Estimate RNA Yield and Absorbance Spectrum
RNA Yield
| HeLa cells | 15 μg RNA/106 cells |
|---|---|
| Jurkat cells | 10 μg RNA/106 cells |
| Vero cells | 15 μg RNA/106 cells |
| Mouse brain | 1.0 μg RNA/mg tissue |
| Mouse liver | 3.5 μg RNA/mg tissue |
| Mouse kidney | 3.0 μg RNA/mg tissue |
| Mouse testis | 1.5 μg RNA/mg tissue |
RNA Extraction from Cultured Cells (HeLa Cells)
RNA Extraction from Cultured Cells (HeLa Cells)
RNA was extracted from HeLa cells (1 x 106 cells), followed by electrophoresis and absorbance measurement.
Electrophoresis
Right lane : Marker 6
1% Agarose S
Absorbance Analysis
| 260/280 | 260/230 | μg/106 cells |
|---|---|---|
| 2.035 ± 0.019 | 2.254 ± 0.028 | 19.30 ± 4.69 |
RNA Extraction from Mouse Liver (Comparison of RNA Quality)
Using the ISOSPIN Cell & Tissue RNA Kit and a competitor’s kit, RNA was extracted from mouse liver according to each manufacturer’s protocol. The quality of the extracted RNA was compared by measuring the RIN values using a Bioanalyzer (Agilent Technologies), as well as by agarose gel electrophoresis and absorbance measurement.
Electrophoresis
![RNA extraction from Mouse liver](/us/product_data/images/02825877_img03.jpg")
Lane➁ : ISOSPIN Cell&Tissue
Extracted RNA was applied each 1 μg.
1% Agarose S
Absorbance Analysis and RIN
| Extraction kit | 260/280 | 260/230 | ng/mg tissue | RIN* |
|---|---|---|---|---|
| Competitor | 2.11 | 1.96 | 1,524 | 7.05 |
| ISOSPIN Cell&Tissue RNA | 2.11 | 2.1 | 4,594 | 7.35 |
* : n=2
Result : High-quality RNA was extracted using the ISOSPIN Cell & Tissue RNA Kit.
FAQ
- Do you have any tips for RNA extraction from adherent cells?
- Please follow the steps below:
- Wash the cells with PBS.
- Add “C Extraction Buffer (For cells)” and pipette to mix.
- Collect the cells using the cell scraper.
- Resuspend the collected cells by pipetting to lyse them.
- Transfer the lysate to a tube.
- No precipitation is observed after centrifugation following the “C Extraction Buffer (For cells)” .
- Depending on the cell type and cell number, precipitation may not be visible. Please collect the supernatant and proceed to the next step.
- How does this kit differ from ISOGEN?
- There are several differences between the two methods. Please select the one that best suits your purpose:
ISOGEN contains phenol and other components that inhibit RNase activity, which allows the sample solution (homogenate) to be frozen and stored during the procedure.
With ISOSPIN Cell & Tissue RNA, the protocol does not allow storage during the process; however, it does not require the use of toxic organic solvents such as phenol or chloroform.
Because ISOGEN recovers RNA by alcohol precipitation after phase separation, the protocol can be easily scaled up.
ISOSPIN Cell & Tissue RNA uses a spin column–based protocol, making it simple to handle and ideal for processing multiple samples.
- Can I extract RNA from a small number of cells?
- It has been tested with 1×104 HeLa cells.
In principle, RNA can also be extracted from fewer cells. If the starting number of cells is low, the RNA can be concentrated by reducing the volume of the elution buffer.
Please note: To ensure that the elution buffer fully contacts the membrane, dispense it onto the center of the membrane. Also, do not reduce the elution volume below 20 µL.
- Can I extract miRNA using this kit?
- miRNAs of 83 bases and 107 bases have been detected by real-time PCR from total RNA samples.
- I would like to extract RNA from heart, skeletal muscle, and cartilage tissues.
- We recommend using the modified protocol with the addition of Proteinase K (not included in the kit).
Overview / Applications
Property
Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.
The prices are list prices in Japan.Please contact your local distributor for your retail price in your region.